Restriction map for
pF1KE0878
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstF5I
BstUI BstXI BstYI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DpnII
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MaeI MaeII MamI
MboI MfeI MflI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI Sau3AI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Alw44I 463 GTGCAC G^TGCAC
ApaLI 463 GTGCAC G^TGCAC
Asp700I 696 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 463 GTGCAC G[AT]GC[AT]^C
AvaII 239 GGACC G^G[AT]CC
BciVI 671 GTATCC GTATCC(6/5)
BlpI 423 GCTCAGC GC^T[ACGT]AGC
BmyI 463 GTGCAC G[AGT]GC[ACT]^C
BpmI 58 CTCCAG CTGGAG(16/14)
Bpu1102I 423 GCTCAGC GC^T[ACGT]AGC
BsaI 308 GGTCTC GGTCTC(1/5)
BsiHKAI 463 GTGCAC G[AT]GC[AT]^C
Bsp1286I 463 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 857 TGTACA T^GTACA
BspHI 479 TCATGA T^CATGA
BspMI 631 ACCTGC ACCTGC(4/8)
BsrGI 857 TGTACA T^GTACA
BsrI 186 ACTGG ACTGG(1/-1)
BstEII 732 GGTGACC G^GT[ACGT]ACC
BstPI 732 GGTGACC G^GT[ACGT]ACC
CelII 423 GCTCAGC GC^T[ACGT]AGC
DsaI 341 CCATGG C^C[GA][CT]GG
EcoO65I 732 GGTGACC G^GT[ACGT]ACC
EcoT14I 341 CCATGG C^C[AT][AT]GG
HincII 486 GTTGAC GT[CT]^[GA]AC
HindII 486 GTTGAC GT[CT]^[GA]AC
MseI 844 TTAA T^TAA
MwoI 209 GCACAACAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 341 CCATGG C^CATGG
NlaIV 238 GGGACC GG[ACGT]^[ACGT]CC
PflMI 452 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 834 GACTC GAGTC(4/5)
RcaI 479 TCATGA T^CATGA
SapI 291 GCTCTTC GCTCTTC(1/4)
ScaI 388 AGTACT AGT^ACT
SfcI 555 CTGTAG C^T[GA][CT]AG
SspBI 857 TGTACA T^GTACA
StuI 698 AGGCCT AGG^CCT
StyI 341 CCATGG C^C[AT][AT]GG
Tru9I 844 TTAA T^TAA
Van91I 452 CCAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 696 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 389 GTAC GT^AC
858 GTAC
BalI 362 TGGCCA TGG^CCA
608 TGGCCA
BbvI 278 GCTGC GCAGC(8/12)
814 GCTGC
BsgI 207 CTGCAC GTGCAG(16/14)
815 CTGCAC
BsmAI 800 GAGAC GTCTC(1/5)
309 GTCTC
BsrDI 129 CATTGC GCAATG(2/0)
539 CATTGC
EaeI 362 TGGCCA [CT]^GGCC[GA]
608 TGGCCA
EarI 292 CTCTTC CTCTTC(1/4)
377 CTCTTC
Fnu4HI 278 GCTGC GC^[ACGT]GC
814 GCTGC
ItaI 278 GCTGC GC^[ACGT]GC
814 GCTGC
Ksp632I 292 CTCTTC CTCTTC(1/4)
377 CTCTTC
MluNI 362 TGGCCA TGG^CCA
608 TGGCCA
MscI 362 TGGCCA TGG^CCA
608 TGGCCA
MslI 406 CATATGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
726 CACAGTGGTG
NdeI 269 CATATG CA^TATG
406 CATATG
RsaI 389 GTAC GT^AC
858 GTAC
TfiI 3 GAATC G^A[AT]TC
569 GAATC
TseI 278 GCTGC G^C[AT]GC
814 GCTGC
TspRI 185 CACTG CA[GC]TG[ACGT][ACGT]^
728 CAGTG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BsmFI BssKI BstNI Cac8I Cfr13I
DdeI EcoRII HaeIII HinfI HphI
MaeIII MboII MnlI MvaI NlaIII
NspI Sau96I ScrFI SfaNI Tsp45I
Tsp509I