Restriction map for
pF1KE0877
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbsI BcgI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr13I ClaI CpoI
DraI DraII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeIII MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 872 GTCTAC GT^[AC][GT]AC
AgeI 362 ACCGGT A^CCGGT
AhdI 450 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 720 GTGCAC G^TGCAC
ApaLI 720 GTGCAC G^TGCAC
AspEI 450 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 321 GGGCTC G[GA]GC[CT]^C
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BciVI 671 GTATCC GTATCC(6/5)
BcnI 6 CCCGG CC^[GC]GG
BfaI 151 CTAG C^TAG
BsaBI 135 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BseRI 219 CTCCTC GAGGAG(10/8)
BspHI 404 TCATGA T^CATGA
BspMI 161 ACCTGC ACCTGC(4/8)
BsrFI 362 ACCGGT [GA]^CCGG[CT]
BsrI 668 CCAGT ACTGG(1/-1)
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
DraIII 327 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 450 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 653 ATGCAT ATGCA^T
MaeI 151 CTAG C^TAG
MaeII 718 ACGT A^CGT
MamI 135 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MwoI 774 GCACCTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 6 CCCGG CC^[GC]GG
NdeI 356 CATATG CA^TATG
NsiI 653 ATGCAT ATGCA^T
PflMI 729 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 362 ACCGGT A^CCGGT
PvuII 434 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SfaNI 515 GCATC GCATC(5/9)
TaqI 699 TCGA T^CGA
TthHB8I 699 TCGA T^CGA
Van91I 729 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 150 TCTAGA T^CTAGA
XcmI 548 CCATCTTGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 788 CCGC CCGC(-3/-1)
85 GCGG
AcsI 539 AAATTC [GA]^AATT[CT]
814 AAATTT
AluI 435 AGCT AG^CT
685 AGCT
ApoI 539 AAATTC [GA]^AATT[CT]
814 AAATTT
AspHI 337 GTGCTC G[AT]GC[AT]^C
720 GTGCAC
BalI 371 TGGCCA TGG^CCA
545 TGGCCA
BbvI 433 GCAGC GCAGC(8/12)
489 GCTGC
BclI 107 TGATCA T^GATCA
602 TGATCA
BsiHKAI 337 GTGCTC G[AT]GC[AT]^C
720 GTGCAC
BsmAI 458 GTCTC GTCTC(1/5)
498 GTCTC
CfoI 229 GCGC GCG^C
290 GCGC
DsaI 317 CCATGG C^C[GA][CT]GG
467 CCATGG
EaeI 371 TGGCCA [CT]^GGCC[GA]
545 TGGCCA
EarI 181 CTCTTC CTCTTC(1/4)
222 CTCTTC
EcoT14I 317 CCATGG C^C[AT][AT]GG
467 CCATGG
FbaI 107 TGATCA T^GATCA
602 TGATCA
Fnu4HI 433 GCAGC GC^[ACGT]GC
489 GCTGC
HhaI 229 GCGC GCG^C
290 GCGC
HinP1I 229 GCGC G^CGC
290 GCGC
ItaI 433 GCAGC GC^[ACGT]GC
489 GCTGC
Ksp632I 181 CTCTTC CTCTTC(1/4)
222 CTCTTC
MluNI 371 TGGCCA TGG^CCA
545 TGGCCA
MscI 371 TGGCCA TGG^CCA
545 TGGCCA
MseI 589 TTAA T^TAA
929 TTAA
MspA1I 434 CAGCTG C[AC]G^C[GT]G
788 CCGCTG
NcoI 317 CCATGG C^CATGG
467 CCATGG
NlaIV 322 GGCTCC GG[ACGT]^[ACGT]CC
439 GGCTCC
SfcI 791 CTGTAG C^T[GA][CT]AG
867 CTATAG
StyI 317 CCATGG C^C[AT][AT]GG
467 CCATGG
Tru9I 589 TTAA T^TAA
929 TTAA
TseI 433 GCAGC G^C[AT]GC
489 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI BmyI BsaJI BsgI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII FokI
HaeIII HapII HinfI HpaII HphI
MboI MboII MnlI MslI MspI
MvaI NdeII NlaIII RsaI Sau3AI
ScrFI TfiI Tsp509I TspRI