Restriction map for
pF1KE0874
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI KspI MamI
MflI MluI MroI MspI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
AflIII 653 ACACGT A^C[GA][CT]GT
AlwI 446 GGATC GGATC(4/5)
AseI 636 ATTAAT AT^TAAT
AsnI 636 ATTAAT AT^TAAT
Asp700I 31 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 85 GTGCTC G[AT]GC[AT]^C
BbvI 433 GCAGC GCAGC(8/12)
BfaI 244 CTAG C^TAG
BmyI 85 GTGCTC G[AGT]GC[ACT]^C
BsiHKAI 85 GTGCTC G[AT]GC[AT]^C
Bsp1286I 85 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 773 TGTACA T^GTACA
BsrGI 773 TGTACA T^GTACA
BsrI 558 ACTGG ACTGG(1/-1)
BssKI 443 CCTGG ^CC[ACGT]GG
BstEII 55 GGTCACC G^GT[ACGT]ACC
BstNI 443 CCTGG CC^[AT]GG
BstPI 55 GGTCACC G^GT[ACGT]ACC
BstXI 20 CCATTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 447 GATC ^GATC
DraII 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 47 GACTTTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 55 GGTCACC G^GT[ACGT]ACC
EcoRII 443 CCTGG ^CC[AT]GG
EcoRV 392 GATATC GAT^ATC
Fnu4HI 433 GCAGC GC^[ACGT]GC
HindIII 707 AAGCTT A^AGCTT
ItaI 433 GCAGC GC^[ACGT]GC
MaeI 244 CTAG C^TAG
MaeII 655 ACGT A^CGT
MboI 447 GATC ^GATC
MfeI 493 CAATTG C^AATTG
MspA1I 434 CAGCTG C[AC]G^C[GT]G
MunI 493 CAATTG C^AATTG
MvaI 443 CCTGG CC^[AT]GG
NdeII 447 GATC ^GATC
PleI 861 GACTC GAGTC(4/5)
PpuMI 439 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 735 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 636 ATTAAT AT^TAAT
PvuII 434 CAGCTG CAG^CTG
SapI 686 GCTCTTC GCTCTTC(1/4)
Sau3AI 447 GATC ^GATC
ScrFI 443 CCTGG CC^[ACGT]GG
SmlI 67 CTTGAG C^T[CT][GA]AG
SspBI 773 TGTACA T^GTACA
SspI 747 AATATT AAT^ATT
TfiI 873 GAATC G^A[AT]TC
TseI 433 GCAGC G^C[AT]GC
Tsp45I 56 GTCAC ^GT[GC]AC
XmnI 31 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 31 GAATTT [GA]^AATT[CT]
539 AAATTC
ApoI 31 GAATTT [GA]^AATT[CT]
539 AAATTC
BalI 314 TGGCCA TGG^CCA
602 TGGCCA
BsgI 290 GTGCAG GTGCAG(16/14)
295 GTGCAG
BsiYI 306 CCTCGGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCTATGTGG
BslI 306 CCTCGGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCTATGTGG
BsmAI 796 GAGAC GTCTC(1/5)
646 GTCTC
BspHI 404 TCATGA T^CATGA
551 TCATGA
DsaI 317 CCATGG C^C[GA][CT]GG
428 CCATGG
EaeI 314 TGGCCA [CT]^GGCC[GA]
602 TGGCCA
EarI 687 CTCTTC CTCTTC(1/4)
766 CTCTTC
EcoT14I 317 CCATGG C^C[AT][AT]GG
428 CCATGG
HinfI 861 GACTC G^A[ACGT]TC
873 GAATC
Ksp632I 687 CTCTTC CTCTTC(1/4)
766 CTCTTC
MaeIII 56 GTCAC ^GT[ACGT]AC
72 GTTAC
MluNI 314 TGGCCA TGG^CCA
602 TGGCCA
MscI 314 TGGCCA TGG^CCA
602 TGGCCA
NcoI 317 CCATGG C^CATGG
428 CCATGG
PstI 467 CTGCAG CTGCA^G
504 CTGCAG
RcaI 404 TCATGA T^CATGA
551 TCATGA
StyI 317 CCATGG C^C[AT][AT]GG
428 CCATGG
TspRI 293 CAGTG CA[GC]TG[ACGT][ACGT]^
479 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI AluI AvaII BsaJI BsmFI
BstF5I Cfr13I DdeI FokI HaeIII
HphI MboII MnlI MseI MslI
NlaIII NlaIV RsaI Sau96I SfaNI
SfcI Tru9I Tsp509I