Restriction map for
pF1KE0873
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AlwI AocI Aor51HI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT14I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MflI MluI MroI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Alw44I 447 GTGCAC G^TGCAC
ApaLI 447 GTGCAC G^TGCAC
Asp700I 680 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 447 GTGCAC G[AT]GC[AT]^C
AvaII 758 GGACC G^G[AT]CC
BalI 371 TGGCCA TGG^CCA
BbsI 826 GTCTTC GAAGAC(2/6)
BbvCI 318 CCTCAGC CCTCAGC(-5/-2)
BfaI 244 CTAG C^TAG
BpuAI 826 GTCTTC GAAGAC(2/6)
BsaI 438 GGTCTC GGTCTC(1/5)
BsgI 163 CTGCAC GTGCAG(16/14)
BsiHKAI 447 GTGCAC G[AT]GC[AT]^C
BsmAI 439 GTCTC GTCTC(1/5)
Bsp1407I 176 TGTACA T^GTACA
BspHI 911 TCATGA T^CATGA
BspMI 251 ACCTGC ACCTGC(4/8)
BsrGI 176 TGTACA T^GTACA
BstAPI 69 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 588 GGTGACC G^GT[ACGT]ACC
BstF5I 600 CATCC GGATG(2/0)
BstPI 588 GGTGACC G^GT[ACGT]ACC
BstXI 425 CCAGGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 323 GCGC GCG^C
DraIII 327 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 140 CCGTGG C^C[GA][CT]GG
EcoO65I 588 GGTGACC G^GT[ACGT]ACC
EcoT22I 406 ATGCAT ATGCA^T
FauI 543 CCCGC CCCGC(4/6)
FokI 600 CATCC GGATG(9/13)
HaeII 322 AGCGCC [GA]GCGC^[CT]
HapII 699 CCGG C^CGG
HhaI 323 GCGC GCG^C
HinP1I 323 GCGC G^CGC
HpaII 699 CCGG C^CGG
MaeI 244 CTAG C^TAG
MaeII 220 ACGT A^CGT
MfeI 608 CAATTG C^AATTG
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MseI 521 TTAA T^TAA
MspA1I 37 CAGCTG C[AC]G^C[GT]G
MspI 699 CCGG C^CGG
MunI 608 CAATTG C^AATTG
NsiI 406 ATGCAT ATGCA^T
PstI 378 CTGCAG CTGCA^G
PvuII 37 CAGCTG CAG^CTG
RcaI 911 TCATGA T^CATGA
SexAI 424 ACCAGGT A^CC[AT]GGT
SfiI 345 GGCCTTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 873 CTTGAG C^T[CT][GA]AG
SspBI 176 TGTACA T^GTACA
Tru9I 521 TTAA T^TAA
Tsp509I 609 AATT ^AATT
XcmI 425 CCAGGTTGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 680 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 390 CCGC CCGC(-3/-1)
544 CCGC
AlwNI 70 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
151 CAGGGCCTG
ApaI 508 GGGCCC GGGCC^C
646 GGGCCC
BbvI 380 GCAGC GCAGC(8/12)
555 GCAGC
BglI 286 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
346 GCCTTGATGGC
BpmI 187 CTCCAG CTGGAG(16/14)
205 CTGGAG
BspLU11I 179 ACATGT A^CATGT
718 ACATGT
DdeI 319 CTCAG C^T[ACGT]AG
584 CTGAG
DpnII 765 GATC ^GATC
816 GATC
EaeI 371 TGGCCA [CT]^GGCC[GA]
696 TGGCCG
Fnu4HI 380 GCAGC GC^[ACGT]GC
555 GCAGC
HinfI 47 GATTC G^A[ACGT]TC
680 GAATC
ItaI 380 GCAGC GC^[ACGT]GC
555 GCAGC
MboI 765 GATC ^GATC
816 GATC
NdeII 765 GATC ^GATC
816 GATC
NspI 179 ACATGT [GA]CATG^[CT]
718 ACATGT
Sau3AI 765 GATC ^GATC
816 GATC
SfaNI 192 GCATC GCATC(5/9)
408 GCATC
SfcI 378 CTGCAG C^T[GA][CT]AG
867 CTACAG
TfiI 47 GATTC G^A[AT]TC
680 GAATC
TseI 380 GCAGC G^C[AT]GC
555 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AflIII AluI BanII BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DraII EcoO109I EcoRII HaeIII HphI
MaeIII MboII MnlI MslI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI Tsp45I TspRI