Restriction map for
pF1KE0872
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccII AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspDI BspEI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstPI
BstUI BstXI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaII KasI Ksp632I KspI
MaeII MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tth111I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 419 GGTACC G^GTACC
AccI 883 GTATAC GT^[AC][GT]AC
AflIII 772 ACATGT A^C[GA][CT]GT
AlwI 700 GGATC GGATC(4/5)
Asp718I 419 GGTACC G^GTACC
BalI 425 TGGCCA TGG^CCA
BanI 419 GGTACC G^G[CT][GA]CC
BbsI 926 GTCTTC GAAGAC(2/6)
BbvI 128 GCTGC GCAGC(8/12)
BglII 263 AGATCT A^GATCT
BpuAI 926 GTCTTC GAAGAC(2/6)
BsaBI 666 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 194 GTGCAG GTGCAG(16/14)
BsmAI 798 GTCTC GTCTC(1/5)
BsmI 860 GAATGC GAATGC(1/-1)
Bsp1407I 230 TGTACA T^GTACA
BspHI 458 TCATGA T^CATGA
BspLU11I 772 ACATGT A^CATGT
BsrDI 892 GCAATG GCAATG(2/0)
BsrGI 230 TGTACA T^GTACA
Bst1107I 883 GTATAC GTA^TAC
BstBI 929 TTCGAA TT^CGAA
BstZ17I 883 GTATAC GTA^TAC
Cac8I 616 GCCTGC GC[ACGT]^[ACGT]GC
DraIII 580 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 105 CCATGG C^C[GA][CT]GG
EaeI 425 TGGCCA [CT]^GGCC[GA]
EcoT14I 105 CCATGG C^C[AT][AT]GG
EcoT22I 70 ATGCAT ATGCA^T
HincII 972 GTTAAC GT[CT]^[GA]AC
HindII 972 GTTAAC GT[CT]^[GA]AC
HindIII 761 AAGCTT A^AGCTT
HpaI 972 GTTAAC GTT^AAC
HphI 822 GGTGA GGTGA(8/7)
KpnI 419 GGTACC GGTAC^C
MamI 666 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 425 TGGCCA TGG^CCA
MscI 425 TGGCCA TGG^CCA
MwoI 497 GCTGGGTAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 105 CCATGG C^CATGG
NsiI 70 ATGCAT ATGCA^T
NspV 929 TTCGAA TT^CGAA
PflMI 783 CCACCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 458 TCATGA T^CATGA
SfcI 921 CTATAG C^T[GA][CT]AG
SfuI 929 TTCGAA TT^CGAA
SspBI 230 TGTACA T^GTACA
StyI 105 CCATGG C^C[AT][AT]GG
TseI 128 GCTGC G^C[AT]GC
Van91I 783 CCACCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 258 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 213 GCGG CCGC(-3/-1)
506 GCGG
ApaI 562 GGGCCC GGGCC^C
679 GGGCCC
AvaII 52 GGACC G^G[AT]CC
779 GGTCC
BstF5I 144 CATCC GGATG(2/0)
234 CATCC
BstYI 263 AGATCT [GA]^GATC[CT]
700 GGATCT
Fnu4HI 128 GCTGC GC^[ACGT]GC
213 GCGGC
FokI 144 CATCC GGATG(9/13)
234 CATCC
HinfI 897 GACTC G^A[ACGT]TC
962 GAGTC
ItaI 128 GCTGC GC^[ACGT]GC
213 GCGGC
MflI 263 AGATCT [GA]^GATC[CT]
700 GGATCT
MslI 222 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
888 CACAGCAATG
NspI 293 ACATGC [GA]CATG^[CT]
772 ACATGT
PleI 897 GACTC GAGTC(4/5)
962 GAGTC
PpuMI 51 GGGACCC [GA]G^G[AT]CC[CT]
778 GGGTCCC
Tsp45I 79 GTGAC ^GT[GC]AC
590 GTGAC
XhoII 263 AGATCT [GA]^GATC[CT]
700 GGATCT
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI BanII BfaI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII HaeIII
MaeI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I Tsp509I TspRI TthHB8I