Restriction map for
pF1KE0871
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BcgI BcnI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FokI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 16 GTAGAC GT^[AC][GT]AC
AflII 21 CTTAAG C^TTAAG
AspHI 636 GAGCTC G[AT]GC[AT]^C
BalI 401 TGGCCA TGG^CCA
BamHI 824 GGATCC G^GATCC
BanII 636 GAGCTC G[GA]GC[CT]^C
BbvI 721 GCAGC GCAGC(8/12)
BciVI 493 GGATAC GTATCC(6/5)
BclI 552 TGATCA T^GATCA
BfrI 21 CTTAAG C^TTAAG
BglII 239 AGATCT A^GATCT
BpmI 325 CTCCAG CTGGAG(16/14)
BseRI 875 CTCCTC GAGGAG(10/8)
BsiHKAI 636 GAGCTC G[AT]GC[AT]^C
BsmAI 365 GTCTC GTCTC(1/5)
Bsp1407I 665 TGTACA T^GTACA
BspDI 392 ATCGAT AT^CGAT
BspHI 434 TCATGA T^CATGA
BsrGI 665 TGTACA T^GTACA
ClaI 392 ATCGAT AT^CGAT
DraII 710 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 49 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 401 TGGCCA [CT]^GGCC[GA]
EcoO109I 710 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 956 GAATTC G^AATTC
FbaI 552 TGATCA T^GATCA
Fnu4HI 721 GCAGC GC^[ACGT]GC
HincII 277 GTCAAC GT[CT]^[GA]AC
HindII 277 GTCAAC GT[CT]^[GA]AC
HphI 126 GGTGA GGTGA(8/7)
ItaI 721 GCAGC GC^[ACGT]GC
MluNI 401 TGGCCA TGG^CCA
MscI 401 TGGCCA TGG^CCA
MwoI 376 GCAGTAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 818 CATATG CA^TATG
NspI 269 ACATGC [GA]CATG^[CT]
PleI 873 GACTC GAGTC(4/5)
PpuMI 710 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 434 TCATGA T^CATGA
SacI 636 GAGCTC GAGCT^C
SfaNI 179 GATGC GCATC(5/9)
SpeI 658 ACTAGT A^CTAGT
SspBI 665 TGTACA T^GTACA
TseI 721 GCAGC G^C[AT]GC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BfaI 274 CTAG C^TAG
659 CTAG
BmyI 596 GTGCCC G[AGT]GC[ACT]^C
636 GAGCTC
BsmFI 787 GGGAC GGGAC(10/14)
712 GTCCC
Bsp1286I 596 GTGCCC G[AGT]GC[ACT]^C
636 GAGCTC
DdeI 632 CTCAG C^T[ACGT]AG
931 CTGAG
EarI 109 CTCTTC CTCTTC(1/4)
902 CTCTTC
EcoT14I 296 CCAAGG C^C[AT][AT]GG
489 CCTTGG
HaeIII 300 GGCC GG^CC
402 GGCC
Ksp632I 109 CTCTTC CTCTTC(1/4)
902 CTCTTC
MaeI 274 CTAG C^TAG
659 CTAG
MseI 22 TTAA T^TAA
884 TTAA
MspA1I 698 CAGCTG C[AC]G^C[GT]G
722 CAGCTG
PstI 418 CTGCAG CTGCA^G
719 CTGCAG
PvuII 698 CAGCTG CAG^CTG
722 CAGCTG
SfcI 418 CTGCAG C^T[GA][CT]AG
719 CTGCAG
SmlI 21 CTTAAG C^T[CT][GA]AG
235 CTTGAG
StyI 296 CCAAGG C^C[AT][AT]GG
489 CCTTGG
TaqI 393 TCGA T^CGA
675 TCGA
Tru9I 22 TTAA T^TAA
884 TTAA
Tsp45I 55 GTGAC ^GT[GC]AC
566 GTGAC
TthHB8I 393 TCGA T^CGA
675 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BsaJI BsiYI BslI BsrI
BssKI BstNI BstYI Cfr13I DpnII
EcoRII HinfI MaeIII MboI MboII
MflI MnlI MslI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TfiI Tsp509I TspRI XhoII