Restriction map for
pF1KE0868
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MseI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 4 GAATTT [GA]^AATT[CT]
AlwI 654 GATCC GGATC(4/5)
AlwNI 891 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 846 CCTGAGG CC^T[ACGT]AGG
ApoI 4 GAATTT [GA]^AATT[CT]
BanI 204 GGTGCC G^G[CT][GA]CC
BbsI 240 GAAGAC GAAGAC(2/6)
BclI 83 TGATCA T^GATCA
BcnI 678 CCGGG CC^[GC]GG
BfaI 586 CTAG C^TAG
BpmI 762 CTCCAG CTGGAG(16/14)
BpuAI 240 GAAGAC GAAGAC(2/6)
BseRI 373 CTCCTC GAGGAG(10/8)
Bsp1407I 803 TGTACA T^GTACA
BspMI 691 ACCTGC ACCTGC(4/8)
BsrGI 803 TGTACA T^GTACA
BstAPI 561 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 143 CATCC GGATG(2/0)
BstXI 266 CCACCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 653 AGATCC [GA]^GATC[CT]
Bsu36I 846 CCTGAGG CC^T[ACGT]AGG
Cac8I 667 GCTGGC GC[ACGT]^[ACGT]GC
DsaI 323 CCATGG C^C[GA][CT]GG
EarI 696 CTCTTC CTCTTC(1/4)
Eco81I 846 CCTGAGG CC^T[ACGT]AGG
EcoT22I 526 ATGCAT ATGCA^T
FbaI 83 TGATCA T^GATCA
FokI 143 CATCC GGATG(9/13)
HapII 678 CCGG C^CGG
HpaII 678 CCGG C^CGG
HphI 614 TCACC GGTGA(8/7)
Ksp632I 696 CTCTTC CTCTTC(1/4)
MaeI 586 CTAG C^TAG
MaeIII 548 GTCAC ^GT[ACGT]AC
MflI 653 AGATCC [GA]^GATC[CT]
MspI 678 CCGG C^CGG
NciI 678 CCGGG CC^[GC]GG
NcoI 323 CCATGG C^CATGG
NsiI 526 ATGCAT ATGCA^T
NspI 528 GCATGT [GA]CATG^[CT]
PflMI 210 CCATATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 695 GCTCTTC GCTCTTC(1/4)
SfaNI 412 GCATC GCATC(5/9)
SmlI 649 CTCAAG C^T[CT][GA]AG
SspBI 803 TGTACA T^GTACA
Tsp45I 548 GTCAC ^GT[GC]AC
Tsp509I 5 AATT ^AATT
Van91I 210 CCATATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 653 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 336 CCGC CCGC(-3/-1)
672 CCGC
AluI 760 AGCT AG^CT
766 AGCT
AspHI 496 GAGCAC G[AT]GC[AT]^C
539 GTGCTC
BalI 218 TGGCCA TGG^CCA
344 TGGCCA
BsiHKAI 496 GAGCAC G[AT]GC[AT]^C
539 GTGCTC
BspHI 377 TCATGA T^CATGA
555 TCATGA
BsrDI 285 CATTGC GCAATG(2/0)
591 CATTGC
Cfr13I 680 GGGCC G^G[ACGT]CC
869 GGGCC
EcoT14I 57 CCTTGG C^C[AT][AT]GG
323 CCATGG
HinfI 387 GACTC G^A[ACGT]TC
546 GAGTC
MluNI 218 TGGCCA TGG^CCA
344 TGGCCA
MscI 218 TGGCCA TGG^CCA
344 TGGCCA
PleI 387 GACTC GAGTC(4/5)
546 GAGTC
PstI 194 CTGCAG CTGCA^G
721 CTGCAG
RcaI 377 TCATGA T^CATGA
555 TCATGA
Sau96I 680 GGGCC G^G[ACGT]CC
869 GGGCC
StyI 57 CCTTGG C^C[AT][AT]GG
323 CCATGG
XcmI 245 CCATCACTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
657 CCACTCGGCTGCTGG
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
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AfaI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
DdeI DpnII EaeI EcoRII Fnu4HI
HaeIII ItaI MboI MboII MnlI
MslI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI ScrFI SfcI
TseI TspRI