Restriction map for
pF1KE0867
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccIII AciI
AclI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BanII BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI HincII HindII HindIII HinfI
HpaI KpnI KspI MaeII MluNI
MroI MscI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 580 CGCG CG^CG
AcsI 922 AAATTC [GA]^AATT[CT]
AcyI 701 GGCGCC G[GA]^CG[CT]C
AfaI 177 GTAC GT^AC
AflIII 579 ACGCGT A^C[GA][CT]GT
AlwNI 70 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 922 AAATTC [GA]^AATT[CT]
BamHI 683 GGATCC G^GATCC
BbeI 701 GGCGCC GGCGC^C
BcnI 816 CCGGG CC^[GC]GG
BfaI 205 CTAG C^TAG
BglII 521 AGATCT A^GATCT
BsaBI 642 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 701 GGCGCC G[GA]^CG[CT]C
BsaWI 686 TCCGGT [AT]^CCGG[AT]
BsmFI 783 GTCCC GGGAC(10/14)
BspHI 407 TCATGA T^CATGA
BspMI 721 ACCTGC ACCTGC(4/8)
BstUI 580 CGCG CG^CG
DraI 855 TTTAAA TTT^AAA
DrdI 58 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 726 CTCTTC CTCTTC(1/4)
HaeII 701 GGCGCC [GA]GCGC^[CT]
Hin1I 701 GGCGCC G[GA]^CG[CT]C
KasI 701 GGCGCC G^GCGCC
Ksp632I 726 CTCTTC CTCTTC(1/4)
MaeI 205 CTAG C^TAG
MamI 642 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 131 CAATTG C^AATTG
MluI 579 ACGCGT A^CGCGT
MunI 131 CAATTG C^AATTG
MvnI 580 CGCG CG^CG
NarI 701 GGCGCC GG^CGCC
NciI 816 CCGGG CC^[GC]GG
NspI 542 ACATGC [GA]CATG^[CT]
RcaI 407 TCATGA T^CATGA
RsaI 177 GTAC GT^AC
SapI 725 GCTCTTC GCTCTTC(1/4)
SfoI 701 GGCGCC GGC^GCC
TspRI 470 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 204 TCTAGA T^CTAGA
XcmI 227 CCATCACCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 684 GATCC GGATC(4/5)
683 GGATC
AocI 61 CCTGAGG CC^T[ACGT]AGG
903 CCTAAGG
BanI 295 GGCACC G^G[CT][GA]CC
701 GGCGCC
BbvI 432 GCTGC GCAGC(8/12)
761 GCTGC
BsgI 762 CTGCAC GTGCAG(16/14)
569 GTGCAG
BsrI 366 CCAGT ACTGG(1/-1)
788 CCAGT
BstF5I 627 CATCC GGATG(2/0)
289 GGATG
BstNI 186 CCTGG CC^[AT]GG
561 CCTGG
BstYI 521 AGATCT [GA]^GATC[CT]
683 GGATCC
Bsu36I 61 CCTGAGG CC^T[ACGT]AGG
903 CCTAAGG
CfoI 558 GCGC GCG^C
702 GCGC
Eco81I 61 CCTGAGG CC^T[ACGT]AGG
903 CCTAAGG
EcoRII 186 CCTGG ^CC[AT]GG
561 CCTGG
EcoT14I 242 CCTTGG C^C[AT][AT]GG
446 CCTTGG
Fnu4HI 432 GCTGC GC^[ACGT]GC
761 GCTGC
FokI 627 CATCC GGATG(9/13)
289 GGATG
HapII 687 CCGG C^CGG
816 CCGG
HhaI 558 GCGC GCG^C
702 GCGC
HinP1I 558 GCGC G^CGC
702 GCGC
HpaII 687 CCGG C^CGG
816 CCGG
ItaI 432 GCTGC GC^[ACGT]GC
761 GCTGC
MflI 521 AGATCT [GA]^GATC[CT]
683 GGATCC
MseI 487 TTAA T^TAA
856 TTAA
MspI 687 CCGG C^CGG
816 CCGG
MvaI 186 CCTGG CC^[AT]GG
561 CCTGG
PstI 548 CTGCAG CTGCA^G
751 CTGCAG
StyI 242 CCTTGG C^C[AT][AT]GG
446 CCTTGG
Tru9I 487 TTAA T^TAA
856 TTAA
TseI 432 GCTGC G^C[AT]GC
761 GCTGC
Tsp509I 132 AATT ^AATT
923 AATT
XhoII 521 AGATCT [GA]^GATC[CT]
683 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AvaII BbsI BpuAI BsaJI
BsiYI BslI BssKI Cac8I Cfr13I
DdeI DpnII HaeIII HphI MaeIII
MboI MboII MnlI MslI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SfcI Tsp45I