Restriction map for
pF1KE0865
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRV EcoT22I
FauI FbaI FseI FspI HapII
HgaI Hin1I HindIII HpaII KasI
KpnI KspI MaeII MluI MroI
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AocI 837 CCTGAGG CC^T[ACGT]AGG
AspHI 350 GTGCTC G[AT]GC[AT]^C
BalI 323 TGGCCA TGG^CCA
BfaI 166 CTAG C^TAG
BglII 59 AGATCT A^GATCT
BmyI 350 GTGCTC G[AGT]GC[ACT]^C
BsaBI 300 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 268 GAGACC GGTCTC(1/5)
BsiHKAI 350 GTGCTC G[AT]GC[AT]^C
BsmAI 268 GAGAC GTCTC(1/5)
Bsp1286I 350 GTGCTC G[AGT]GC[ACT]^C
BsrDI 182 GCAATG GCAATG(2/0)
BsrI 489 ACTGG ACTGG(1/-1)
BstEII 84 GGTCACC G^GT[ACGT]ACC
BstPI 84 GGTCACC G^GT[ACGT]ACC
BstYI 59 AGATCT [GA]^GATC[CT]
Bsu36I 837 CCTGAGG CC^T[ACGT]AGG
Cfr13I 135 GGCCC G^G[ACGT]CC
EaeI 323 TGGCCA [CT]^GGCC[GA]
EarI 353 CTCTTC CTCTTC(1/4)
Eco81I 837 CCTGAGG CC^T[ACGT]AGG
EcoO65I 84 GGTCACC G^GT[ACGT]ACC
EcoRI 91 GAATTC G^AATTC
HaeII 568 AGCGCC [GA]GCGC^[CT]
HincII 640 GTTAAC GT[CT]^[GA]AC
HindII 640 GTTAAC GT[CT]^[GA]AC
HinfI 741 GATTC G^A[ACGT]TC
HpaI 640 GTTAAC GTT^AAC
Ksp632I 353 CTCTTC CTCTTC(1/4)
MaeI 166 CTAG C^TAG
MamI 300 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 506 CAATTG C^AATTG
MflI 59 AGATCT [GA]^GATC[CT]
MluNI 323 TGGCCA TGG^CCA
MscI 323 TGGCCA TGG^CCA
MseI 641 TTAA T^TAA
MunI 506 CAATTG C^AATTG
MwoI 496 GCTGCCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 684 GGTTCC GG[ACGT]^[ACGT]CC
SapI 352 GCTCTTC GCTCTTC(1/4)
Sau96I 135 GGCCC G^G[ACGT]CC
SfaNI 300 GATGC GCATC(5/9)
SfcI 845 CTACAG C^T[GA][CT]AG
StuI 841 AGGCCT AGG^CCT
TfiI 741 GATTC G^A[AT]TC
Tru9I 641 TTAA T^TAA
XhoII 59 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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BbvCI 746 CCTCAGC CCTCAGC(-5/-2)
751 GCTGAGG
BsmI 251 GCATTC GAATGC(1/-1)
719 GCATTC
BspMI 778 ACCTGC ACCTGC(4/8)
474 GCAGGT
BssKI 210 CCTGG ^CC[ACGT]GG
326 CCAGG
BstF5I 204 CATCC GGATG(2/0)
945 GGATG
BstNI 210 CCTGG CC^[AT]GG
326 CCAGG
CfoI 569 GCGC GCG^C
782 GCGC
DpnII 60 GATC ^GATC
421 GATC
DsaI 80 CCATGG C^C[GA][CT]GG
920 CCATGG
EcoRII 210 CCTGG ^CC[AT]GG
326 CCAGG
FokI 204 CATCC GGATG(9/13)
945 GGATG
HhaI 569 GCGC GCG^C
782 GCGC
HinP1I 569 GCGC G^CGC
782 GCGC
MboI 60 GATC ^GATC
421 GATC
MvaI 210 CCTGG CC^[AT]GG
326 CCAGG
NcoI 80 CCATGG C^CATGG
920 CCATGG
NdeII 60 GATC ^GATC
421 GATC
NlaIII 81 CATG CATG^
921 CATG
Sau3AI 60 GATC ^GATC
421 GATC
ScrFI 210 CCTGG CC^[ACGT]GG
326 CCAGG
TspRI 198 CAGTG CA[GC]TG[ACGT][ACGT]^
795 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BsaJI BsiYI BslI Cac8I DdeI
EcoT14I Fnu4HI HaeIII HphI ItaI
MaeIII MboII MnlI MslI MspA1I
PvuII RsaI StyI TseI Tsp45I
Tsp509I