Restriction map for
pF1KE0864
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HinfI
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 844 GTCTAC GT^[AC][GT]AC
AcsI 76 GAATTC [GA]^AATT[CT]
AcyI 949 GACGCC G[GA]^CG[CT]C
AflIII 668 ACGTGT A^C[GA][CT]GT
AlwI 123 GATCC GGATC(4/5)
AocI 888 CCTTAGG CC^T[ACGT]AGG
ApoI 76 GAATTC [GA]^AATT[CT]
BbsI 19 GTCTTC GAAGAC(2/6)
BcnI 720 CCGGG CC^[GC]GG
BpuAI 19 GTCTTC GAAGAC(2/6)
BsaAI 667 TACGTG [CT]AC^GT[GA]
BsaHI 949 GACGCC G[GA]^CG[CT]C
BsgI 400 CTGCAC GTGCAG(16/14)
BsmAI 661 GTCTC GTCTC(1/5)
BsmBI 660 CGTCTC CGTCTC(1/5)
BspHI 416 TCATGA T^CATGA
BsrI 27 CCAGT ACTGG(1/-1)
Bsu36I 888 CCTTAGG CC^T[ACGT]AGG
EaeI 519 CGGCCA [CT]^GGCC[GA]
Eco81I 888 CCTTAGG CC^T[ACGT]AGG
EcoRI 76 GAATTC G^AATTC
HapII 720 CCGG C^CGG
Hin1I 949 GACGCC G[GA]^CG[CT]C
HpaII 720 CCGG C^CGG
MaeII 668 ACGT A^CGT
MseI 496 TTAA T^TAA
MspA1I 397 CCGCTG C[AC]G^C[GT]G
MspI 720 CCGG C^CGG
NciI 720 CCGGG CC^[GC]GG
PflMI 143 CCATCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 416 TCATGA T^CATGA
Tru9I 496 TTAA T^TAA
Tsp509I 77 AATT ^AATT
Van91I 143 CCATCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 143 CCATCATCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 244 GTAC GT^AC
789 GTAC
AluI 428 AGCT AG^CT
485 AGCT
AvaII 756 GGTCC G^G[AT]CC
803 GGTCC
BspMI 733 ACCTGC ACCTGC(4/8)
791 ACCTGC
BssSI 628 CTCGTG CACGAG(-5/-1)
784 CTCGTG
BstXI 260 CCAACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
284 CCATTTCGTTGG
CfoI 570 GCGC GCG^C
581 GCGC
DpnII 123 GATC ^GATC
360 GATC
DsaI 236 CCGTGG C^C[GA][CT]GG
752 CCGTGG
FauI 396 CCCGC CCCGC(4/6)
422 CCCGC
HhaI 570 GCGC GCG^C
581 GCGC
HinP1I 570 GCGC G^CGC
581 GCGC
MaeIII 325 GTCAC ^GT[ACGT]AC
856 GTCAC
MboI 123 GATC ^GATC
360 GATC
NdeII 123 GATC ^GATC
360 GATC
NlaIII 363 CATG CATG^
417 CATG
RsaI 244 GTAC GT^AC
789 GTAC
Sau3AI 123 GATC ^GATC
360 GATC
Tsp45I 325 GTCAC ^GT[GC]AC
856 GTCAC
TspRI 39 CACTG CA[GC]TG[ACGT][ACGT]^
72 CACTG
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI Alw44I AlwNI ApaI
ApaLI AspHI BanII BbvI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HgaI HphI ItaI Ksp632I MboII
MnlI MslI MvaI MvnI MwoI
NlaIV PstI Sau96I ScrFI SfaNI
SfcI TseI