Restriction map for
pF1KE0863
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MseI MspA1I MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI Tru9I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 439 GGTACC G^GTACC
AccI 100 GTCTAC GT^[AC][GT]AC
AccII 817 CGCG CG^CG
AcsI 31 GAATTC [GA]^AATT[CT]
AluI 209 AGCT AG^CT
AlwI 627 GATCC GGATC(4/5)
ApoI 31 GAATTC [GA]^AATT[CT]
Asp718I 439 GGTACC G^GTACC
AspHI 208 GAGCTC G[AT]GC[AT]^C
AspI 814 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 239 GGACC G^G[AT]CC
BclI 131 TGATCA T^GATCA
BfaI 679 CTAG C^TAG
BglI 421 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 590 AGATCT A^GATCT
BsaI 744 GGTCTC GGTCTC(1/5)
BsiEI 784 CGGCCG CG[GA][CT]^CG
BsiHKAI 208 GAGCTC G[AT]GC[AT]^C
BspMI 721 ACCTGC ACCTGC(4/8)
BsrDI 682 GCAATG GCAATG(2/0)
BsrI 550 CCAGT ACTGG(1/-1)
BssSI 739 CTCGTG CACGAG(-5/-1)
BstEII 108 GGTCACC G^GT[ACGT]ACC
BstPI 108 GGTCACC G^GT[ACGT]ACC
BstUI 817 CGCG CG^CG
BstXI 236 CCAGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 590 AGATCT [GA]^GATC[CT]
DraIII 910 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 784 CGGCCG C^GGCCG
EclXI 784 CGGCCG C^GGCCG
Eco52I 784 CGGCCG C^GGCCG
EcoNI 141 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 108 GGTCACC G^GT[ACGT]ACC
EcoRI 31 GAATTC G^AATTC
FauI 525 CCCGC CCCGC(4/6)
FbaI 131 TGATCA T^GATCA
HaeII 926 AGCGCC [GA]GCGC^[CT]
HapII 783 CCGG C^CGG
HgaI 818 GCGTC GACGC(5/10)
HinfI 47 GATTC G^A[ACGT]TC
HpaII 783 CCGG C^CGG
KpnI 439 GGTACC GGTAC^C
MaeI 679 CTAG C^TAG
MflI 590 AGATCT [GA]^GATC[CT]
MspI 783 CCGG C^CGG
MvnI 817 CGCG CG^CG
PflFI 814 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 238 AGGACCC [GA]G^G[AT]CC[CT]
PstI 67 CTGCAG CTGCA^G
SacI 208 GAGCTC GAGCT^C
SapI 210 GCTCTTC GCTCTTC(1/4)
SbfI 66 CCTGCAGG CCTGCA^GG
ScaI 552 AGTACT AGT^ACT
SexAI 104 ACCTGGT A^CC[AT]GGT
SfcI 67 CTGCAG C^T[GA][CT]AG
SfiI 420 GGCCTGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 66 CCTGCAGG CCTGCA^GG
TfiI 47 GATTC G^A[AT]TC
Tsp509I 32 AATT ^AATT
Tth111I 814 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 674 CCATCCTAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 590 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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ApaI 72 GGGCCC GGGCC^C
899 GGGCCC
BalI 245 TGGCCA TGG^CCA
428 TGGCCA
BanI 439 GGTACC G^G[CT][GA]CC
670 GGTGCC
BsgI 163 CTGCAC GTGCAG(16/14)
293 GTGCAG
BsmAI 745 GTCTC GTCTC(1/5)
827 GTCTC
CfoI 189 GCGC GCG^C
927 GCGC
EcoT14I 353 CCATGG C^C[AT][AT]GG
431 CCATGG
HhaI 189 GCGC GCG^C
927 GCGC
HinP1I 189 GCGC G^CGC
927 GCGC
MaeII 308 ACGT A^CGT
890 ACGT
MluNI 245 TGGCCA TGG^CCA
428 TGGCCA
MscI 245 TGGCCA TGG^CCA
428 TGGCCA
MwoI 421 GCCTGCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
673 GCCATCCTAGC
NcoI 353 CCATGG C^CATGG
431 CCATGG
SmlI 58 CTCAAG C^T[CT][GA]AG
408 CTTGAG
StyI 353 CCATGG C^C[AT][AT]GG
431 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI BanII BbsI BbvI
BmyI BpuAI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MslI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI