Restriction map for
pF1KE0861
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I BspDI BspEI BspLU11I BsrBI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DraII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MamI MboI
MflI MluI MroI MspI MvnI
NaeI NarI NciI NdeI NdeII
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PstI PvuI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AclI 300 AACGTT AA^CGTT
AseI 565 ATTAAT AT^TAAT
AsnI 565 ATTAAT AT^TAAT
AvaII 621 GGTCC G^G[AT]CC
BbvI 466 GCAGC GCAGC(8/12)
BsmFI 622 GTCCC GGGAC(10/14)
BspMI 283 GCAGGT ACCTGC(4/8)
BstXI 626 CCATGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 621 GGTCC G^G[ACGT]CC
DraIII 508 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 15 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 466 GCAGC GC^[ACGT]GC
HphI 159 TCACC GGTGA(8/7)
ItaI 466 GCAGC GC^[ACGT]GC
MaeII 301 ACGT A^CGT
MfeI 330 CAATTG C^AATTG
MunI 330 CAATTG C^AATTG
NheI 199 GCTAGC G^CTAGC
NspI 191 GCATGC [GA]CATG^[CT]
PflMI 363 CCACTATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 565 ATTAAT AT^TAAT
Psp1406I 300 AACGTT AA^CGTT
Sau96I 621 GGTCC G^G[ACGT]CC
SfaNI 240 GATGC GCATC(5/9)
SfcI 797 CTATAG C^T[GA][CT]AG
SphI 191 GCATGC GCATG^C
TseI 466 GCAGC G^C[AT]GC
Van91I 363 CCACTATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 426 CCAGCTGGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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BbsI 702 GAAGAC GAAGAC(2/6)
637 GTCTTC
BbvCI 683 CCTCAGC CCTCAGC(-5/-2)
688 GCTGAGG
BfaI 200 CTAG C^TAG
280 CTAG
BpmI 51 CTCCAG CTGGAG(16/14)
246 CTCCAG
BpuAI 702 GAAGAC GAAGAC(2/6)
637 GTCTTC
Bsp1407I 101 TGTACA T^GTACA
215 TGTACA
BspHI 134 TCATGA T^CATGA
548 TCATGA
BsrDI 765 CATTGC GCAATG(2/0)
122 GCAATG
BsrGI 101 TGTACA T^GTACA
215 TGTACA
DsaI 461 CCATGG C^C[GA][CT]GG
626 CCATGG
HinfI 47 GATTC G^A[ACGT]TC
56 GATTC
MaeI 200 CTAG C^TAG
280 CTAG
MseI 109 TTAA T^TAA
566 TTAA
NcoI 461 CCATGG C^CATGG
626 CCATGG
NlaIV 439 GGAGCC GG[ACGT]^[ACGT]CC
621 GGTCCC
RcaI 134 TCATGA T^CATGA
548 TCATGA
SmlI 387 CTTGAG C^T[CT][GA]AG
673 CTCAAG
SspBI 101 TGTACA T^GTACA
215 TGTACA
TfiI 47 GATTC G^A[AT]TC
56 GATTC
Tru9I 109 TTAA T^TAA
566 TTAA
Tsp509I 331 AATT ^AATT
592 AATT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI BalI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cac8I
DdeI EaeI EcoRII EcoT14I FokI
HaeIII MaeIII MboII MluNI MnlI
MscI MslI MspA1I MvaI MwoI
NlaIII PvuII RsaI ScrFI StyI
Tsp45I TspRI