Restriction map for
pF1KE0860
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII BssKI BssSI Bst1107I
BstBI BstEII BstNI BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRII EcoRV EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HphI ItaI KasI KpnI KspI
MboI MfeI MflI MluI MroI
MunI MvaI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI TaqI TfiI TseI Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
AcsI 31 GAATTC [GA]^AATT[CT]
ApoI 31 GAATTC [GA]^AATT[CT]
Asp700I 917 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 440 GGTCC G^G[AT]CC
BbsI 702 GAAGAC GAAGAC(2/6)
BpuAI 702 GAAGAC GAAGAC(2/6)
BsaBI 636 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 278 CCTTGG C^C[ACGT][ACGT]GG
BsmBI 208 GAGACG CGTCTC(1/5)
BsmFI 414 GGGAC GGGAC(10/14)
BsmI 239 GAATGC GAATGC(1/-1)
Bsp1407I 215 TGTACA T^GTACA
BsrGI 215 TGTACA T^GTACA
BstAPI 283 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 439 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 31 GAATTC G^AATTC
EcoT14I 278 CCTTGG C^C[AT][AT]GG
HapII 696 CCGG C^CGG
HpaII 696 CCGG C^CGG
MaeII 826 ACGT A^CGT
MamI 636 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 109 TTAA T^TAA
MspI 696 CCGG C^CGG
NlaIV 439 GGGTCC GG[ACGT]^[ACGT]CC
NspI 191 GCATGC [GA]CATG^[CT]
PflMI 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 439 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 378 CTGCAG CTGCA^G
SfcI 378 CTGCAG C^T[GA][CT]AG
SmlI 775 CTCAAG C^T[CT][GA]AG
SphI 191 GCATGC GCATG^C
SspBI 215 TGTACA T^GTACA
SspI 919 AATATT AAT^ATT
StuI 630 AGGCCT AGG^CCT
StyI 278 CCTTGG C^C[AT][AT]GG
Tru9I 109 TTAA T^TAA
Van91I 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 426 CCAGCTGGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 917 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 100 GTCTAC GT^[AC][GT]AC
517 GTAGAC
AfaI 216 GTAC GT^AC
828 GTAC
BalI 320 TGGCCA TGG^CCA
371 TGGCCA
BbvCI 683 CCTCAGC CCTCAGC(-5/-2)
688 GCTGAGG
BfaI 53 CTAG C^TAG
200 CTAG
BmyI 422 GTGCCC G[AGT]GC[ACT]^C
622 GTGCCC
BsmAI 208 GAGAC GTCTC(1/5)
721 GTCTC
Bsp1286I 422 GTGCCC G[AGT]GC[ACT]^C
622 GTGCCC
BspHI 404 TCATGA T^CATGA
548 TCATGA
BsrDI 765 CATTGC GCAATG(2/0)
349 GCAATG
BsrI 222 ACTGG ACTGG(1/-1)
544 CCAGT
BstF5I 669 CATCC GGATG(2/0)
891 GGATG
Cfr13I 440 GGTCC G^G[ACGT]CC
693 GGGCC
EaeI 320 TGGCCA [CT]^GGCC[GA]
371 TGGCCA
EarI 663 CTCTTC CTCTTC(1/4)
3 GAAGAG
FokI 669 CATCC GGATG(9/13)
891 GGATG
Ksp632I 663 CTCTTC CTCTTC(1/4)
3 GAAGAG
MaeI 53 CTAG C^TAG
200 CTAG
MluNI 320 TGGCCA TGG^CCA
371 TGGCCA
MscI 320 TGGCCA TGG^CCA
371 TGGCCA
MspA1I 427 CAGCTG C[AC]G^C[GT]G
686 CAGCTG
PvuII 427 CAGCTG CAG^CTG
686 CAGCTG
RcaI 404 TCATGA T^CATGA
548 TCATGA
RsaI 216 GTAC GT^AC
828 GTAC
Sau96I 440 GGTCC G^G[ACGT]CC
693 GGGCC
SfaNI 892 GATGC GCATC(5/9)
66 GCATC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AluI BsiYI BslI Cac8I DdeI
HaeIII MaeIII MboII MnlI MslI
MwoI NlaIII Tsp45I Tsp509I TspRI