Restriction map for
pF1KE0859
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BcgI BclI BcnI BfaI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 12 AAATTC [GA]^AATT[CT]
AflII 679 CTTAAG C^TTAAG
AlwI 442 GGATC GGATC(4/5)
AlwNI 609 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 390 CCTAAGG CC^T[ACGT]AGG
ApoI 12 AAATTC [GA]^AATT[CT]
AvaII 156 GGACC G^G[AT]CC
BbvCI 689 CCTCAGC CCTCAGC(-5/-2)
BbvI 66 GCAGC GCAGC(8/12)
BciVI 425 GTATCC GTATCC(6/5)
BfrI 679 CTTAAG C^TTAAG
BpmI 51 CTCCAG CTGGAG(16/14)
Bsp1407I 867 TGTACA T^GTACA
BspHI 137 TCATGA T^CATGA
BsrGI 867 TGTACA T^GTACA
BstF5I 750 CATCC GGATG(2/0)
Bsu36I 390 CCTAAGG CC^T[ACGT]AGG
CfoI 725 GCGC GCG^C
DpnII 443 GATC ^GATC
DraII 418 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 574 GACACCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 912 CCGTGG C^C[GA][CT]GG
Eco81I 390 CCTAAGG CC^T[ACGT]AGG
EcoNI 804 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 418 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 459 CCTTGG C^C[AT][AT]GG
FokI 750 CATCC GGATG(9/13)
HgaI 611 GCGTC GACGC(5/10)
HhaI 725 GCGC GCG^C
HinP1I 725 GCGC G^CGC
HphI 317 TCACC GGTGA(8/7)
MboI 443 GATC ^GATC
MspA1I 692 CAGCTG C[AC]G^C[GT]G
MwoI 718 GCCACCTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 241 CATATG CA^TATG
NdeII 443 GATC ^GATC
NspI 194 GCATGC [GA]CATG^[CT]
PvuII 692 CAGCTG CAG^CTG
RcaI 137 TCATGA T^CATGA
SapI 303 GCTCTTC GCTCTTC(1/4)
Sau3AI 443 GATC ^GATC
SmlI 679 CTTAAG C^T[CT][GA]AG
SphI 194 GCATGC GCATG^C
SspBI 867 TGTACA T^GTACA
StuI 710 AGGCCT AGG^CCT
StyI 459 CCTTGG C^C[AT][AT]GG
TfiI 261 GAATC G^A[AT]TC
TseI 66 GCAGC G^C[AT]GC
Tsp509I 13 AATT ^AATT
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 366 CCGC CCGC(-3/-1)
63 GCGG
BbsI 79 GTCTTC GAAGAC(2/6)
91 GTCTTC
BpuAI 79 GTCTTC GAAGAC(2/6)
91 GTCTTC
BsaI 211 GAGACC GGTCTC(1/5)
253 GGTCTC
BsgI 567 CTGCAC GTGCAG(16/14)
902 CTGCAC
BsmFI 807 GGGAC GGGAC(10/14)
792 GTCCC
BsrDI 276 CATTGC GCAATG(2/0)
125 GCAATG
BsrI 432 ACTGG ACTGG(1/-1)
447 ACTGG
EarI 304 CTCTTC CTCTTC(1/4)
942 GAAGAG
Fnu4HI 63 GCGGC GC^[ACGT]GC
66 GCAGC
ItaI 63 GCGGC GC^[ACGT]GC
66 GCAGC
Ksp632I 304 CTCTTC CTCTTC(1/4)
942 GAAGAG
MseI 680 TTAA T^TAA
937 TTAA
PleI 114 GACTC GAGTC(4/5)
818 GACTC
Tru9I 680 TTAA T^TAA
937 TTAA
Tsp45I 25 GTGAC ^GT[GC]AC
829 GTGAC
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BalI BsaJI BsiYI
BslI BsmAI BspMI BssKI BstNI
Cac8I Cfr13I DdeI EaeI EcoRII
HaeIII HinfI MaeIII MboII MluNI
MnlI MscI MslI MvaI NlaIII
RsaI Sau96I ScrFI SfaNI TspRI
XcmI