Restriction map for
pF1KE0858
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AlwNI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaHI BsaI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MaeI MfeI MluI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StyI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 236 CGCG CG^CG
AccIII 804 TCCGGA T^CCGGA
AcsI 31 GAATTC [GA]^AATT[CT]
AhdI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 751 GTGCAC G^TGCAC
AocI 876 CCTCAGG CC^T[ACGT]AGG
Aor51HI 266 AGCGCT AGC^GCT
ApaLI 751 GTGCAC G^TGCAC
ApoI 31 GAATTC [GA]^AATT[CT]
AspEI 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 538 CACGTG CAC^GTG
BbsI 909 GAAGAC GAAGAC(2/6)
BbvCI 855 CCTCAGC CCTCAGC(-5/-2)
BcnI 429 CCGGG CC^[GC]GG
BpuAI 909 GAAGAC GAAGAC(2/6)
BsaBI 822 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 804 TCCGGA [AT]^CCGG[AT]
BseAI 804 TCCGGA T^CCGGA
BsgI 420 CTGCAC GTGCAG(16/14)
BsiEI 360 CGACCG CG[GA][CT]^CG
BsmFI 794 GTCCC GGGAC(10/14)
BspEI 804 TCCGGA T^CCGGA
BspHI 404 TCATGA T^CATGA
BstUI 236 CGCG CG^CG
BstXI 590 CCAAAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 876 CCTCAGG CC^T[ACGT]AGG
DraII 792 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 221 CCGTGG C^C[GA][CT]GG
Eam1105I 250 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 266 AGCGCT AGC^GCT
Eco81I 876 CCTCAGG CC^T[ACGT]AGG
EcoO109I 792 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 31 GAATTC G^AATTC
HaeII 266 AGCGCT [GA]GCGC^[CT]
MamI 822 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 804 TCCGGA T^CCGGA
MspA1I 70 CAGCTG C[AC]G^C[GT]G
MvnI 236 CGCG CG^CG
NciI 429 CCGGG CC^[GC]GG
NlaIV 793 GGTCCC GG[ACGT]^[ACGT]CC
PflMI 140 CCACTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 538 CACGTG CAC^GTG
PmlI 538 CACGTG CAC^GTG
PpuMI 792 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 70 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
SapI 78 GCTCTTC GCTCTTC(1/4)
SphI 239 GCATGC GCATG^C
StuI 710 AGGCCT AGG^CCT
Tsp45I 475 GTGAC ^GT[GC]AC
Tsp509I 32 AATT ^AATT
Van91I 140 CCACTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 272 CCATCGCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 102 GTAC GT^AC
177 GTAC
AspHI 400 GTGCTC G[AT]GC[AT]^C
751 GTGCAC
AvaII 793 GGTCC G^G[AT]CC
840 GGTCC
BpmI 67 CTCCAG CTGGAG(16/14)
147 CTGGAG
BsaAI 367 TACGTG [CT]AC^GT[GA]
538 CACGTG
BsaJI 221 CCGTGG C^C[ACGT][ACGT]GG
443 CCTGGG
BseRI 637 CTCCTC GAGGAG(10/8)
646 CTCCTC
BsiHKAI 400 GTGCTC G[AT]GC[AT]^C
751 GTGCAC
BsmAI 812 GAGAC GTCTC(1/5)
801 GTCTC
BsrI 293 CCAGT ACTGG(1/-1)
798 CCAGT
BstNI 282 CCTGG CC^[AT]GG
443 CCTGG
DdeI 856 CTCAG C^T[ACGT]AG
877 CTCAG
EarI 79 CTCTTC CTCTTC(1/4)
181 CTCTTC
EcoRII 282 CCTGG ^CC[AT]GG
443 CCTGG
FauI 234 CCCGC CCCGC(4/6)
414 GCGGG
HapII 429 CCGG C^CGG
805 CCGG
HpaII 429 CCGG C^CGG
805 CCGG
Ksp632I 79 CTCTTC CTCTTC(1/4)
181 CTCTTC
MaeIII 392 GTTAC ^GT[ACGT]AC
475 GTGAC
MspI 429 CCGG C^CGG
805 CCGG
MvaI 282 CCTGG CC^[AT]GG
443 CCTGG
NspI 167 ACATGC [GA]CATG^[CT]
239 GCATGC
RsaI 102 GTAC GT^AC
177 GTAC
SfaNI 75 GATGC GCATC(5/9)
827 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI BalI BbvI
BglI BmyI BsiYI BslI Bsp1286I
BspMI BssKI BstF5I BstYI Cac8I
CfoI Cfr13I DpnII EaeI Fnu4HI
FokI HaeIII HhaI HinP1I HphI
ItaI MaeII MboI MboII MflI
MluNI MnlI MscI MslI MwoI
NdeII NlaIII Sau3AI Sau96I ScrFI
TseI TspRI XhoII