Restriction map for
pF1KE0849
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AviII AvrII BamHI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BclI BcnI BfrI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KspI
MaeII MamI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI TseI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 292 GGTACC G^GTACC
AcsI 31 GAATTT [GA]^AATT[CT]
AlwI 153 GGATC GGATC(4/5)
AocI 873 CCTGAGG CC^T[ACGT]AGG
ApoI 31 GAATTT [GA]^AATT[CT]
Asp700I 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 292 GGTACC G^GTACC
AvaI 61 CCCGAG C^[CT]CG[GA]G
BciVI 570 GGATAC GTATCC(6/5)
BglII 249 AGATCT A^GATCT
BsaWI 451 TCCGGT [AT]^CCGG[AT]
BsmAI 635 GTCTC GTCTC(1/5)
BsoBI 61 CCCGAG C^[CT]CG[GA]G
BspMI 776 ACCTGC ACCTGC(4/8)
BssKI 93 CCTGG ^CC[ACGT]GG
BstNI 93 CCTGG CC^[AT]GG
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
Cac8I 591 GCAAGC GC[ACGT]^[ACGT]GC
DraII 412 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 518 CCGTGG C^C[GA][CT]GG
EarI 748 CTCTTC CTCTTC(1/4)
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoO109I 412 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 93 CCTGG ^CC[AT]GG
FauI 656 CCCGC CCCGC(4/6)
HapII 452 CCGG C^CGG
HinfI 23 GAGTC G^A[ACGT]TC
HpaII 452 CCGG C^CGG
HphI 218 TCACC GGTGA(8/7)
KpnI 292 GGTACC GGTAC^C
Ksp632I 748 CTCTTC CTCTTC(1/4)
MseI 553 TTAA T^TAA
MspI 452 CCGG C^CGG
MvaI 93 CCTGG CC^[AT]GG
MwoI 234 GCCAAAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI 23 GAGTC GAGTC(4/5)
PpuMI 412 AGGTCCC [GA]G^G[AT]CC[CT]
ScrFI 93 CCTGG CC^[ACGT]GG
SfaNI 66 GATGC GCATC(5/9)
SfcI 472 CTACAG C^T[GA][CT]AG
SpeI 558 ACTAGT A^CTAGT
Tru9I 553 TTAA T^TAA
XmnI 31 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AluI 480 AGCT AG^CT
815 AGCT
BanI 231 GGTGCC G^G[CT][GA]CC
292 GGTACC
BstYI 153 GGATCT [GA]^GATC[CT]
249 AGATCT
EcoT14I 221 CCTTGG C^C[AT][AT]GG
262 CCAAGG
Fnu4HI 698 GCCGC GC^[ACGT]GC
780 GCGGC
ItaI 698 GCCGC GC^[ACGT]GC
780 GCGGC
MflI 153 GGATCT [GA]^GATC[CT]
249 AGATCT
MslI 735 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
843 CACACCTATG
StyI 221 CCTTGG C^C[AT][AT]GG
262 CCAAGG
XhoII 153 GGATCT [GA]^GATC[CT]
249 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI AvaII BalI BfaI
BsaJI BsiYI BslI BsmFI BsrI
BstF5I Cfr13I DdeI DpnII EaeI
FokI HaeIII MaeI MaeIII MboI
MboII MluNI MnlI MscI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
Tsp45I Tsp509I TspRI