Restriction map for
pF1KE0848
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsiEI BsiWI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstYI BstZ17I Cac8I CelII
CfoI Cfr10I ClaI CpoI DpnII
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRV EcoT14I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MboI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 31 GAATTC [GA]^AATT[CT]
AflIII 572 ACACGT A^C[GA][CT]GT
AocI 449 CCTTAGG CC^T[ACGT]AGG
ApoI 31 GAATTC [GA]^AATT[CT]
AseI 429 ATTAAT AT^TAAT
AsnI 429 ATTAAT AT^TAAT
Asp700I 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 64 GAGCTC G[AT]GC[AT]^C
BanI 604 GGCACC G^G[CT][GA]CC
BanII 64 GAGCTC G[GA]GC[CT]^C
BmyI 64 GAGCTC G[AGT]GC[ACT]^C
BpmI 67 CTCCAG CTGGAG(16/14)
BsaJI 69 CCAGGG C^C[ACGT][ACGT]GG
BsaWI 801 ACCGGA [AT]^CCGG[AT]
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
BsmBI 144 CGTCTC CGTCTC(1/5)
BsmI 595 GCATTC GAATGC(1/-1)
Bsp1286I 64 GAGCTC G[AGT]GC[ACT]^C
BsrBI 725 CCGCTC CCGCTC(-3/-3)
BstEII 108 GGTGACC G^GT[ACGT]ACC
BstPI 108 GGTGACC G^GT[ACGT]ACC
Bsu36I 449 CCTTAGG CC^T[ACGT]AGG
EarI 891 GAAGAG CTCTTC(1/4)
Eco81I 449 CCTTAGG CC^T[ACGT]AGG
EcoO65I 108 GGTGACC G^GT[ACGT]ACC
EcoRI 31 GAATTC G^AATTC
EcoT22I 593 ATGCAT ATGCA^T
HapII 802 CCGG C^CGG
HinfI 855 GAATC G^A[ACGT]TC
HpaII 802 CCGG C^CGG
Ksp632I 891 GAAGAG CTCTTC(1/4)
MslI 735 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 802 CCGG C^CGG
NsiI 593 ATGCAT ATGCA^T
NspI 562 GCATGT [GA]CATG^[CT]
PshBI 429 ATTAAT AT^TAAT
SacI 64 GAGCTC GAGCT^C
SfaNI 681 GATGC GCATC(5/9)
SfcI 867 CTACAG C^T[GA][CT]AG
StuI 707 AGGCCT AGG^CCT
TaqI 657 TCGA T^CGA
TfiI 855 GAATC G^A[AT]TC
TthHB8I 657 TCGA T^CGA
XmnI 331 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 725 CCGC CCGC(-3/-1)
859 CCGC
AfaI 177 GTAC GT^AC
467 GTAC
AvaII 207 GGACC G^G[AT]CC
249 GGTCC
BbvI 75 GCAGC GCAGC(8/12)
370 GCTGC
BfaI 151 CTAG C^TAG
491 CTAG
BsgI 221 GTGCAG GTGCAG(16/14)
566 GTGCAG
BsiYI 499 CCCTTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
784 CCCAAATCTGG
BslI 499 CCCTTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
784 CCCAAATCTGG
BsmAI 699 GAGAC GTCTC(1/5)
145 GTCTC
BsrI 694 ACTGG ACTGG(1/-1)
764 CCAGT
BssKI 69 CCAGG ^CC[ACGT]GG
93 CCTGG
BstNI 69 CCAGG CC^[AT]GG
93 CCTGG
EcoRII 69 CCAGG ^CC[AT]GG
93 CCTGG
MaeI 151 CTAG C^TAG
491 CTAG
MaeII 167 ACGT A^CGT
574 ACGT
MaeIII 109 GTGAC ^GT[ACGT]AC
742 GTGAC
MvaI 69 CCAGG CC^[AT]GG
93 CCTGG
NlaIV 604 GGCACC GG[ACGT]^[ACGT]CC
624 GGTTCC
RsaI 177 GTAC GT^AC
467 GTAC
ScrFI 69 CCAGG CC^[ACGT]GG
93 CCTGG
TseI 75 GCAGC G^C[AT]GC
370 GCTGC
Tsp45I 109 GTGAC ^GT[GC]AC
742 GTGAC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BstF5I Cfr13I DdeI Fnu4HI
FokI HaeIII HphI ItaI MboII
MnlI MseI MwoI NlaIII Sau96I
Tru9I Tsp509I TspRI