Restriction map for
pF1KE0845
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII KasI KpnI KspI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflII 772 CTTAAG C^TTAAG
AlwI 675 GATCC GGATC(4/5)
AlwNI 55 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 2 TGGCCA TGG^CCA
BanII 451 GGGCTC G[GA]GC[CT]^C
BbsI 205 GTCTTC GAAGAC(2/6)
BfaI 277 CTAG C^TAG
BfrI 772 CTTAAG C^TTAAG
BpuAI 205 GTCTTC GAAGAC(2/6)
BsaBI 327 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 377 GTCCC GGGAC(10/14)
BspHI 398 TCATGA T^CATGA
BspMI 586 GCAGGT ACCTGC(4/8)
BstYI 674 AGATCC [GA]^GATC[CT]
Cfr13I 440 GGGCC G^G[ACGT]CC
EaeI 2 TGGCCA [CT]^GGCC[GA]
EarI 82 CTCTTC CTCTTC(1/4)
EcoT14I 14 CCTTGG C^C[AT][AT]GG
HincII 241 GTCAAC GT[CT]^[GA]AC
HindII 241 GTCAAC GT[CT]^[GA]AC
HindIII 701 AAGCTT A^AGCTT
Ksp632I 82 CTCTTC CTCTTC(1/4)
MaeI 277 CTAG C^TAG
MaeII 149 ACGT A^CGT
MamI 327 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 674 AGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 473 CAGCTG C[AC]G^C[GT]G
MwoI 466 GCGTACACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 917 CATATG CA^TATG
NlaIV 502 GGCTCC GG[ACGT]^[ACGT]CC
PleI 410 GAGTC GAGTC(4/5)
PstI 907 CTGCAG CTGCA^G
PvuII 473 CAGCTG CAG^CTG
RcaI 398 TCATGA T^CATGA
SapI 81 GCTCTTC GCTCTTC(1/4)
Sau96I 440 GGGCC G^G[ACGT]CC
SfaNI 834 GATGC GCATC(5/9)
SmlI 772 CTTAAG C^T[CT][GA]AG
StyI 14 CCTTGG C^C[AT][AT]GG
TfiI 513 GATTC G^A[AT]TC
XcmI 5 CCAATGTCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 674 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AcsI 232 AAATTC [GA]^AATT[CT]
786 AAATTC
ApoI 232 AAATTC [GA]^AATT[CT]
786 AAATTC
AspHI 79 GTGCTC G[AT]GC[AT]^C
890 GTGCTC
BsiHKAI 79 GTGCTC G[AT]GC[AT]^C
890 GTGCTC
BsiYI 443 CCATTGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
493 CCTTTCTGTGG
BslI 443 CCATTGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
493 CCTTTCTGTGG
BstF5I 201 GGATG GGATG(2/0)
280 GGATG
Cac8I 475 GCTGGC GC[ACGT]^[ACGT]GC
866 GCCTGC
DdeI 315 CTCAG C^T[ACGT]AG
547 CTAAG
FokI 201 GGATG GGATG(9/13)
280 GGATG
HaeIII 3 GGCC GG^CC
441 GGCC
HinfI 410 GAGTC G^A[ACGT]TC
513 GATTC
NspI 604 GCATGT [GA]CATG^[CT]
712 ACATGC
SfcI 861 CTACAG C^T[GA][CT]AG
907 CTGCAG
Tsp45I 10 GTCAC ^GT[GC]AC
19 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AfaI AluI BbvI BmyI
BpmI BsaJI Bsp1286I BsrI BssKI
BstNI DpnII EcoRII Fnu4HI HphI
ItaI MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
RsaI Sau3AI ScrFI Tru9I TseI
Tsp509I TspRI