Restriction map for
pF1KE0844
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstUI BstXI BstYI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 874 GTATAC GT^[AC][GT]AC
AlwI 404 GGATC GGATC(4/5)
AlwNI 529 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 717 GAGCAC G[AT]GC[AT]^C
BanI 286 GGCACC G^G[CT][GA]CC
BanII 577 GGGCTC G[GA]GC[CT]^C
BciVI 493 GGATAC GTATCC(6/5)
BclI 317 TGATCA T^GATCA
BcnI 8 CCCGG CC^[GC]GG
BpmI 330 CTCCAG CTGGAG(16/14)
BsiHKAI 717 GAGCAC G[AT]GC[AT]^C
BsmFI 184 GTCCC GGGAC(10/14)
BspHI 854 TCATGA T^CATGA
BsrDI 541 GCAATG GCAATG(2/0)
Bst1107I 874 GTATAC GTA^TAC
BstZ17I 874 GTATAC GTA^TAC
Cac8I 537 GCGTGC GC[ACGT]^[ACGT]GC
EcoT14I 602 CCATGG C^C[AT][AT]GG
FauI 633 GCGGG CCCGC(4/6)
FbaI 317 TGATCA T^GATCA
HaeIII 3 GGCC GG^CC
HapII 9 CCGG C^CGG
HpaII 9 CCGG C^CGG
MspI 9 CCGG C^CGG
NciI 8 CCCGG CC^[GC]GG
NcoI 602 CCATGG C^CATGG
RcaI 854 TCATGA T^CATGA
SfaNI 701 GCATC GCATC(5/9)
SmlI 300 CTTGAG C^T[CT][GA]AG
StyI 602 CCATGG C^C[AT][AT]GG
XcmI 785 CCAATAGTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 171 CCGC CCGC(-3/-1)
633 GCGG
AvaII 119 GGTCC G^G[AT]CC
636 GGTCC
BbsI 307 GTCTTC GAAGAC(2/6)
729 GTCTTC
BmyI 577 GGGCTC G[AGT]GC[ACT]^C
717 GAGCAC
BpuAI 307 GTCTTC GAAGAC(2/6)
729 GTCTTC
Bsp1286I 577 GGGCTC G[AGT]GC[ACT]^C
717 GAGCAC
BspMI 292 ACCTGC ACCTGC(4/8)
110 GCAGGT
BstAPI 101 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
287 GCACCACCTGC
BstF5I 702 CATCC GGATG(2/0)
708 CATCC
Cfr13I 119 GGTCC G^G[ACGT]CC
636 GGTCC
DdeI 162 CTTAG C^T[ACGT]AG
838 CTCAG
DraIII 102 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
533 CACTGCGTG
DsaI 44 CCGTGG C^C[GA][CT]GG
602 CCATGG
FokI 702 CATCC GGATG(9/13)
708 CATCC
HinfI 53 GAGTC G^A[ACGT]TC
182 GAGTC
MaeIII 445 GTGAC ^GT[ACGT]AC
863 GTGAC
MspA1I 171 CCGCTG C[AC]G^C[GT]G
631 CAGCGG
NlaIV 286 GGCACC GG[ACGT]^[ACGT]CC
635 GGGTCC
NspI 375 GCATGT [GA]CATG^[CT]
413 GCATGT
PflMI 189 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
258 CCATAGAGTGG
PleI 53 GAGTC GAGTC(4/5)
182 GAGTC
Sau96I 119 GGTCC G^G[ACGT]CC
636 GGTCC
Tsp45I 445 GTGAC ^GT[GC]AC
863 GTGAC
Van91I 189 CCAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
258 CCATAGAGTGG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
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AluI BbvI BsaJI BsgI BsiYI
BslI BsrI BssKI BstNI DpnII
EcoRII Fnu4HI HphI ItaI MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII Sau3AI ScrFI
Tru9I TseI TspRI