Restriction map for
pF1KE0841
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsiYI BslI BsmAI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrI BssHII
BssSI BstAPI BstBI BstUI BstXI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SphI Sse8387I SspI StuI
SwaI TspRI Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 816 GTATAC GT^[AC][GT]AC
AciI 488 GCGG CCGC(-3/-1)
AlwNI 321 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 831 ATTAAT AT^TAAT
AsnI 831 ATTAAT AT^TAAT
AspHI 542 GTGCTC G[AT]GC[AT]^C
AvaI 89 CCCGGG C^[CT]CG[GA]G
AvaII 86 GGTCC G^G[AT]CC
BalI 743 TGGCCA TGG^CCA
BglI 211 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 542 GTGCTC G[AGT]GC[ACT]^C
BpmI 526 CTGGAG CTGGAG(16/14)
BsaAI 480 CACGTA [CT]AC^GT[GA]
BsiHKAI 542 GTGCTC G[AT]GC[AT]^C
BsmFI 87 GTCCC GGGAC(10/14)
BsoBI 89 CCCGGG C^[CT]CG[GA]G
Bsp1286I 542 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 101 TGTACA T^GTACA
BsrDI 207 CATTGC GCAATG(2/0)
BsrGI 101 TGTACA T^GTACA
Bst1107I 816 GTATAC GTA^TAC
BstEII 809 GGTTACC G^GT[ACGT]ACC
BstPI 809 GGTTACC G^GT[ACGT]ACC
BstYI 55 GGATCT [GA]^GATC[CT]
BstZ17I 816 GTATAC GTA^TAC
Cac8I 129 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 86 GGTCC G^G[ACGT]CC
DraI 987 TTTAAA TTT^AAA
DraII 85 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 743 TGGCCA [CT]^GGCC[GA]
EcoO109I 85 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 809 GGTTACC G^GT[ACGT]ACC
EcoT14I 943 CCTTGG C^C[AT][AT]GG
HapII 90 CCGG C^CGG
HpaII 90 CCGG C^CGG
HphI 428 TCACC GGTGA(8/7)
MaeII 481 ACGT A^CGT
MflI 55 GGATCT [GA]^GATC[CT]
MluNI 743 TGGCCA TGG^CCA
MscI 743 TGGCCA TGG^CCA
MslI 453 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 90 CCGG C^CGG
PpuMI 85 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 831 ATTAAT AT^TAAT
PvuII 181 CAGCTG CAG^CTG
Sau96I 86 GGTCC G^G[ACGT]CC
SmaI 89 CCCGGG CCC^GGG
SpeI 697 ACTAGT A^CTAGT
SspBI 101 TGTACA T^GTACA
StyI 943 CCTTGG C^C[AT][AT]GG
TaqI 380 TCGA T^CGA
TthHB8I 380 TCGA T^CGA
XhoII 55 GGATCT [GA]^GATC[CT]
XmaCI 89 CCCGGG C^CCGGG
XmaI 89 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 403 GATCC GGATC(4/5)
55 GGATC
BcnI 89 CCCGG CC^[GC]GG
90 CCGGG
BsgI 184 CTGCAC GTGCAG(16/14)
28 GTGCAG
BstF5I 716 CATCC GGATG(2/0)
45 GGATG
DpnII 56 GATC ^GATC
403 GATC
EarI 314 CTCTTC CTCTTC(1/4)
348 CTCTTC
FokI 716 CATCC GGATG(9/13)
45 GGATG
HaeIII 219 GGCC GG^CC
744 GGCC
Ksp632I 314 CTCTTC CTCTTC(1/4)
348 CTCTTC
MboI 56 GATC ^GATC
403 GATC
MspA1I 181 CAGCTG C[AC]G^C[GT]G
486 CAGCGG
NciI 89 CCCGG CC^[GC]GG
90 CCGGG
NdeII 56 GATC ^GATC
403 GATC
Sau3AI 56 GATC ^GATC
403 GATC
Tsp45I 244 GTGAC ^GT[GC]AC
781 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BssKI BstNI DdeI
EcoRII Fnu4HI HinfI ItaI MaeI
MaeIII MboII MnlI MseI MvaI
MwoI NlaIII NlaIV RsaI ScrFI
SfaNI TfiI Tru9I TseI Tsp509I