Restriction map for
pF1KE0838
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BanI BanII BbeI
BbrPI BbvCI BcgI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrGI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT22I FauI FseI
FspI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaI
HphI KasI KpnI Ksp632I KspI
MaeII MamI MluI MluNI MroI
MscI MslI MspA1I MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI StuI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 901 ACATGT A^C[GA][CT]GT
Alw44I 388 GTGCAC G^TGCAC
ApaLI 388 GTGCAC G^TGCAC
AspHI 388 GTGCAC G[AT]GC[AT]^C
BamHI 395 GGATCC G^GATCC
BbsI 313 GTCTTC GAAGAC(2/6)
BciVI 73 GGATAC GTATCC(6/5)
BclI 173 TGATCA T^GATCA
BglII 616 AGATCT A^GATCT
BmyI 388 GTGCAC G[AGT]GC[ACT]^C
BpmI 41 CTGGAG CTGGAG(16/14)
BpuAI 313 GTCTTC GAAGAC(2/6)
BsaI 668 GAGACC GGTCTC(1/5)
BsaJI 833 CCTTGG C^C[ACGT][ACGT]GG
BsiHKAI 388 GTGCAC G[AT]GC[AT]^C
BsmFI 574 GTCCC GGGAC(10/14)
BsmI 893 GAATGC GAATGC(1/-1)
Bsp1286I 388 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 901 ACATGT A^CATGT
BspMI 301 ACCTGC ACCTGC(4/8)
BsrDI 78 CATTGC GCAATG(2/0)
BsrFI 658 ACCGGC [GA]^CCGG[CT]
Cac8I 916 GCCTGC GC[ACGT]^[ACGT]GC
Cfr10I 658 ACCGGC [GA]^CCGG[CT]
DraI 861 TTTAAA TTT^AAA
EcoRI 46 GAATTC G^AATTC
EcoRV 594 GATATC GAT^ATC
EcoT14I 833 CCTTGG C^C[AT][AT]GG
FbaI 173 TGATCA T^GATCA
HapII 659 CCGG C^CGG
HpaII 659 CCGG C^CGG
MspI 659 CCGG C^CGG
NlaIV 395 GGATCC GG[ACGT]^[ACGT]CC
NspI 901 ACATGT [GA]CATG^[CT]
PshAI 882 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 11 CTGCAG CTGCA^G
SfaNI 407 GATGC GCATC(5/9)
SfcI 11 CTGCAG C^T[GA][CT]AG
SpeI 84 ACTAGT A^CTAGT
SspI 60 AATATT AAT^ATT
StyI 833 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 46 GAATTC [GA]^AATT[CT]
164 GAATTT
AfaI 208 GTAC GT^AC
451 GTAC
AluI 769 AGCT AG^CT
795 AGCT
AlwI 396 GATCC GGATC(4/5)
395 GGATC
ApoI 46 GAATTC [GA]^AATT[CT]
164 GAATTT
BbvI 442 GCAGC GCAGC(8/12)
817 GCAGC
BfaI 55 CTAG C^TAG
85 CTAG
BsmAI 627 GAGAC GTCTC(1/5)
668 GAGAC
BstYI 395 GGATCC [GA]^GATC[CT]
616 AGATCT
DdeI 454 CTGAG C^T[ACGT]AG
874 CTGAG
Fnu4HI 442 GCAGC GC^[ACGT]GC
817 GCAGC
HincII 88 GTCAAC GT[CT]^[GA]AC
130 GTTGAC
HindII 88 GTCAAC GT[CT]^[GA]AC
130 GTTGAC
ItaI 442 GCAGC GC^[ACGT]GC
817 GCAGC
MaeI 55 CTAG C^TAG
85 CTAG
MaeIII 588 GTCAC ^GT[ACGT]AC
839 GTCAC
MfeI 479 CAATTG C^AATTG
569 CAATTG
MflI 395 GGATCC [GA]^GATC[CT]
616 AGATCT
MnlI 341 CCTC CCTC(7/6)
876 GAGG
MunI 479 CAATTG C^AATTG
569 CAATTG
RsaI 208 GTAC GT^AC
451 GTAC
ScaI 207 AGTACT AGT^ACT
450 AGTACT
TseI 442 GCAGC G^C[AT]GC
817 GCAGC
Tsp45I 588 GTCAC ^GT[GC]AC
839 GTCAC
XcmI 349 CCACTTTTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
416 CCATTTCCTTGTTGG
XhoII 395 GGATCC [GA]^GATC[CT]
616 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BsrI BstF5I DpnII FokI MboI
MboII MseI NdeII NlaIII Sau3AI
Tru9I Tsp509I TspRI