Restriction map for
pF1KE0837
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaJI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MaeII MaeIII MamI MfeI
MluI MroI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tsp45I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AciI 624 GCGG CCGC(-3/-1)
AlwNI 440 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 69 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 152 TGGCCA TGG^CCA
BanII 695 GAGCCC G[GA]GC[CT]^C
BbvCI 306 CCTCAGC CCTCAGC(-5/-2)
BmyI 695 GAGCCC G[AGT]GC[ACT]^C
BpmI 765 CTCCAG CTGGAG(16/14)
BsaI 668 GAGACC GGTCTC(1/5)
BseRI 724 CTCCTC GAGGAG(10/8)
BsiYI 852 CCTAATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 852 CCTAATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 784 GAGACG CGTCTC(1/5)
Bsp1286I 695 GAGCCC G[AGT]GC[ACT]^C
BspMI 301 ACCTGC ACCTGC(4/8)
BsrDI 756 CATTGC GCAATG(2/0)
BstF5I 362 GGATG GGATG(2/0)
BstYI 59 GGATCT [GA]^GATC[CT]
DraIII 657 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 152 TGGCCA [CT]^GGCC[GA]
EarI 111 GAAGAG CTCTTC(1/4)
FokI 362 GGATG GGATG(9/13)
HaeIII 153 GGCC GG^CC
Ksp632I 111 GAAGAG CTCTTC(1/4)
MflI 59 GGATCT [GA]^GATC[CT]
MluNI 152 TGGCCA TGG^CCA
MscI 152 TGGCCA TGG^CCA
PflFI 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 811 GAGTC GAGTC(4/5)
SfaNI 883 GCATC GCATC(5/9)
SmlI 834 CTCAAG C^T[CT][GA]AG
Tth111I 100 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 349 CCACTTTTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 59 GGATCT [GA]^GATC[CT]
XmnI 69 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 612 GATCC GGATC(4/5)
59 GGATC
BbvI 645 GCAGC GCAGC(8/12)
570 GCTGC
BsgI 13 GTGCAG GTGCAG(16/14)
663 GTGCAG
BssKI 392 CCTGG ^CC[ACGT]GG
561 CCTGG
BstNI 392 CCTGG CC^[AT]GG
561 CCTGG
DpnII 60 GATC ^GATC
612 GATC
EcoRII 392 CCTGG ^CC[AT]GG
561 CCTGG
EcoT22I 71 ATGCAT ATGCA^T
881 ATGCAT
Fnu4HI 570 GCTGC GC^[ACGT]GC
645 GCAGC
ItaI 570 GCTGC GC^[ACGT]GC
645 GCAGC
MboI 60 GATC ^GATC
612 GATC
MvaI 392 CCTGG CC^[AT]GG
561 CCTGG
MwoI 639 GCGAATGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
697 GCCCTGAAAGC
NdeII 60 GATC ^GATC
612 GATC
NsiI 71 ATGCAT ATGCA^T
881 ATGCAT
NspI 534 ACATGC [GA]CATG^[CT]
879 ACATGC
Sau3AI 60 GATC ^GATC
612 GATC
ScrFI 392 CCTGG CC^[ACGT]GG
561 CCTGG
TfiI 248 GAATC G^A[AT]TC
396 GATTC
TseI 570 GCTGC G^C[AT]GC
645 GCAGC
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
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AcsI AluI ApoI BsmAI BsmI
BsrI DdeI HinfI HphI MboII
MnlI MseI NlaIII Tru9I Tsp509I
TspRI