Restriction map for
pF1KE0836
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AfaI 274 GTAC GT^AC
AlwI 25 GGATC GGATC(4/5)
AlwNI 894 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 692 CCTCAGG CC^T[ACGT]AGG
AvaII 326 GGTCC G^G[AT]CC
AvrII 183 CCTAGG C^CTAGG
BlnI 183 CCTAGG C^CTAGG
BpmI 453 CTCCAG CTGGAG(16/14)
BsaI 8 GAGACC GGTCTC(1/5)
BseRI 745 CTCCTC GAGGAG(10/8)
BsmAI 8 GAGAC GTCTC(1/5)
BsmI 309 GAATGC GAATGC(1/-1)
BspMI 802 GCAGGT ACCTGC(4/8)
BsrDI 101 GCAATG GCAATG(2/0)
BstEII 60 GGTTACC G^GT[ACGT]ACC
BstPI 60 GGTTACC G^GT[ACGT]ACC
BstXI 50 CCATCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 25 GGATCT [GA]^GATC[CT]
Bsu36I 692 CCTCAGG CC^T[ACGT]AGG
DpnII 26 GATC ^GATC
DraI 826 TTTAAA TTT^AAA
DraII 187 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 424 CCATGG C^C[GA][CT]GG
Eco81I 692 CCTCAGG CC^T[ACGT]AGG
EcoO109I 187 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 60 GGTTACC G^GT[ACGT]ACC
MboI 26 GATC ^GATC
MflI 25 GGATCT [GA]^GATC[CT]
MslI 630 CATTTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 424 CCATGG C^CATGG
NdeII 26 GATC ^GATC
PflMI 317 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 274 GTAC GT^AC
Sau3AI 26 GATC ^GATC
SmlI 936 CTCAAG C^T[CT][GA]AG
TfiI 698 GATTC G^A[AT]TC
Van91I 317 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 25 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 571 AAATTC [GA]^AATT[CT]
829 AAATTC
ApoI 571 AAATTC [GA]^AATT[CT]
829 AAATTC
BanII 448 GGGCTC G[GA]GC[CT]^C
706 GAGCCC
BmyI 448 GGGCTC G[AGT]GC[ACT]^C
706 GAGCCC
BsgI 715 GTGCAG GTGCAG(16/14)
863 GTGCAG
BsmFI 299 GGGAC GGGAC(10/14)
946 GGGAC
Bsp1286I 448 GGGCTC G[AGT]GC[ACT]^C
706 GAGCCC
BssKI 84 CCTGG ^CC[ACGT]GG
335 CCTGG
BstF5I 247 CATCC GGATG(2/0)
428 GGATG
BstNI 84 CCTGG CC^[AT]GG
335 CCTGG
Cfr13I 188 GGGCC G^G[ACGT]CC
326 GGTCC
EcoRII 84 CCTGG ^CC[AT]GG
335 CCTGG
FokI 247 CATCC GGATG(9/13)
428 GGATG
MseI 511 TTAA T^TAA
827 TTAA
MvaI 84 CCTGG CC^[AT]GG
335 CCTGG
NlaIV 187 GGGGCC GG[ACGT]^[ACGT]CC
515 GGAACC
PleI 844 GAGTC GAGTC(4/5)
924 GAGTC
Sau96I 188 GGGCC G^G[ACGT]CC
326 GGTCC
ScrFI 84 CCTGG CC^[ACGT]GG
335 CCTGG
SfaNI 429 GATGC GCATC(5/9)
246 GCATC
Tru9I 511 TTAA T^TAA
827 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI BbsI BbvI BfaI BpuAI
BsaJI BsiYI BslI BsrI Cac8I
DdeI EarI EcoT14I Fnu4HI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboII MnlI MwoI NlaIII
PstI SapI SfcI StyI TseI
Tsp45I Tsp509I TspRI