Restriction map for
pF1KE0833
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI KspI
MaeII MamI MboI MflI MluI
MluNI MroI MscI MseI MspI
MvnI NaeI NarI NciI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 357 CCGC CCGC(-3/-1)
BbsI 658 GTCTTC GAAGAC(2/6)
BfaI 424 CTAG C^TAG
BpuAI 658 GTCTTC GAAGAC(2/6)
BseRI 144 CTCCTC GAGGAG(10/8)
BsiEI 354 CGACCG CG[GA][CT]^CG
BsmI 452 GCATTC GAATGC(1/-1)
Bsp1407I 335 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BspMI 119 ACCTGC ACCTGC(4/8)
BsrGI 335 TGTACA T^GTACA
BsrI 538 CCAGT ACTGG(1/-1)
BstAPI 277 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 506 CCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 433 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 321 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 428 CCATGG C^C[GA][CT]GG
EarI 295 CTCTTC CTCTTC(1/4)
EcoO109I 433 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 25 GAATTC G^AATTC
EcoRV 532 GATATC GAT^ATC
EcoT14I 428 CCATGG C^C[AT][AT]GG
Ksp632I 295 CTCTTC CTCTTC(1/4)
MaeI 424 CTAG C^TAG
MaeIII 374 GTCAC ^GT[ACGT]AC
MfeI 487 CAATTG C^AATTG
MspA1I 618 CAGCTG C[AC]G^C[GT]G
MunI 487 CAATTG C^AATTG
NcoI 428 CCATGG C^CATGG
PflMI 306 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 433 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 618 CAGCTG CAG^CTG
RcaI 398 TCATGA T^CATGA
SapI 294 GCTCTTC GCTCTTC(1/4)
SfcI 645 CTACAG C^T[GA][CT]AG
SspBI 335 TGTACA T^GTACA
StyI 428 CCATGG C^C[AT][AT]GG
Tsp45I 374 GTCAC ^GT[GC]AC
Van91I 306 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 6 GAATTT [GA]^AATT[CT]
25 GAATTC
AfaI 171 GTAC GT^AC
336 GTAC
ApoI 6 GAATTT [GA]^AATT[CT]
25 GAATTC
AvaII 78 GGTCC G^G[AT]CC
434 GGACC
BbvCI 569 CCTCAGC CCTCAGC(-5/-2)
615 CCTCAGC
BbvI 317 GCTGC GCAGC(8/12)
449 GCTGC
BpmI 61 CTGGAG CTGGAG(16/14)
577 CTGGAG
BsgI 318 CTGCAC GTGCAG(16/14)
560 GTGCAG
BsmAI 64 GAGAC GTCTC(1/5)
142 GTCTC
BsmFI 433 GGGAC GGGAC(10/14)
502 GTCCC
BssKI 312 CCTGG ^CC[ACGT]GG
576 CCTGG
BstNI 312 CCTGG CC^[AT]GG
576 CCTGG
EcoRII 312 CCTGG ^CC[AT]GG
576 CCTGG
Fnu4HI 317 GCTGC GC^[ACGT]GC
449 GCTGC
ItaI 317 GCTGC GC^[ACGT]GC
449 GCTGC
MvaI 312 CCTGG CC^[AT]GG
576 CCTGG
NlaIV 116 GGAACC GG[ACGT]^[ACGT]CC
433 GGGACC
RsaI 171 GTAC GT^AC
336 GTAC
ScrFI 312 CCTGG CC^[ACGT]GG
576 CCTGG
TseI 317 GCTGC G^C[AT]GC
449 GCTGC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BstF5I
Cac8I Cfr13I DdeI FokI HaeIII
HphI MboII MnlI MslI MwoI
NlaIII Sau96I SfaNI Tsp509I TspRI