Restriction map for
pF1KE0831
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# NO CUT ENZYMES ( 173 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbrPI
BbsI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HapII HgaI HincII HindII
HindIII HpaI HpaII KpnI KspI
MfeI MluI MroI MseI MspA1I
MspI MunI MvnI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 150 GACGTC GACGT^C
AlwI 436 GGATC GGATC(4/5)
Asp700I 746 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 334 GGACC G^G[AT]CC
BalI 197 TGGCCA TGG^CCA
BanI 524 GGCGCC G^G[CT][GA]CC
BbeI 524 GGCGCC GGCGC^C
BbvCI 12 CCTCAGC CCTCAGC(-5/-2)
BbvI 562 GCTGC GCAGC(8/12)
BsaBI 641 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstYI 436 GGATCT [GA]^GATC[CT]
Cac8I 42 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 334 GGACC G^G[ACGT]CC
DsaI 176 CCATGG C^C[GA][CT]GG
EaeI 197 TGGCCA [CT]^GGCC[GA]
EarI 429 CTCTTC CTCTTC(1/4)
EcoRV 645 GATATC GAT^ATC
EcoT14I 176 CCATGG C^C[AT][AT]GG
Fnu4HI 562 GCTGC GC^[ACGT]GC
HaeII 524 GGCGCC [GA]GCGC^[CT]
ItaI 562 GCTGC GC^[ACGT]GC
KasI 524 GGCGCC G^GCGCC
Ksp632I 429 CTCTTC CTCTTC(1/4)
MamI 641 GATTGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 436 GGATCT [GA]^GATC[CT]
MluNI 197 TGGCCA TGG^CCA
MscI 197 TGGCCA TGG^CCA
MslI 597 CATTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 172 GCGACCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 524 GGCGCC GG^CGCC
NcoI 176 CCATGG C^CATGG
NlaIV 524 GGCGCC GG[ACGT]^[ACGT]CC
PleI 318 GAGTC GAGTC(4/5)
Sau96I 334 GGACC G^G[ACGT]CC
SfoI 524 GGCGCC GGC^GCC
SpeI 304 ACTAGT A^CTAGT
StuI 533 AGGCCT AGG^CCT
StyI 176 CCATGG C^C[AT][AT]GG
TfiI 77 GATTC G^A[AT]TC
TseI 562 GCTGC G^C[AT]GC
Tsp509I 414 AATT ^AATT
TspRI 662 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 436 GGATCT [GA]^GATC[CT]
XmnI 746 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 515 CCGC CCGC(-3/-1)
565 GCGG
AcyI 150 GACGTC G[GA]^CG[CT]C
524 GGCGCC
AfaI 3 GTAC GT^AC
548 GTAC
AluI 112 AGCT AG^CT
125 AGCT
BsaHI 150 GACGTC G[GA]^CG[CT]C
524 GGCGCC
BsiYI 515 CCGCCGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
558 CCTCGCTGCGG
BslI 515 CCGCCGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
558 CCTCGCTGCGG
BsmFI 148 GGGAC GGGAC(10/14)
234 GTCCC
BsmI 157 GAATGC GAATGC(1/-1)
288 GAATGC
BsrI 168 ACTGG ACTGG(1/-1)
617 CCAGT
BssKI 213 CCTGG ^CC[ACGT]GG
273 CCTGG
BstNI 213 CCTGG CC^[AT]GG
273 CCTGG
CfoI 510 GCGC GCG^C
525 GCGC
EcoRII 213 CCTGG ^CC[AT]GG
273 CCTGG
HhaI 510 GCGC GCG^C
525 GCGC
Hin1I 150 GACGTC G[GA]^CG[CT]C
524 GGCGCC
HinP1I 510 GCGC G^CGC
525 GCGC
HinfI 77 GATTC G^A[ACGT]TC
318 GAGTC
MaeII 87 ACGT A^CGT
151 ACGT
MboII 504 GAAGA GAAGA(8/7)
430 TCTTC
MvaI 213 CCTGG CC^[AT]GG
273 CCTGG
NlaIII 141 CATG CATG^
177 CATG
RsaI 3 GTAC GT^AC
548 GTAC
ScrFI 213 CCTGG CC^[ACGT]GG
273 CCTGG
SfaNI 507 GATGC GCATC(5/9)
606 GATGC
SfcI 134 CTGTAG C^T[GA][CT]AG
693 CTATAG
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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BfaI BsaJI BstF5I DdeI DpnII
FokI HaeIII HphI MaeI MaeIII
MboI MnlI NdeII Sau3AI Tsp45I