Restriction map for
pF1KE0826
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# NO CUT ENZYMES ( 203 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MfeI MluI MroI
MseI MspI MunI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
ScrFI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AciI 660 CCGC CCGC(-3/-1)
AfaI 171 GTAC GT^AC
BalI 2 TGGCCA TGG^CCA
BmyI 619 GTGCCC G[AGT]GC[ACT]^C
BsiYI 493 CCTTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 493 CCTTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 619 GTGCCC G[AGT]GC[ACT]^C
BspHI 398 TCATGA T^CATGA
BstF5I 666 CATCC GGATG(2/0)
BstYI 674 AGATCC [GA]^GATC[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoT14I 51 CCTTGG C^C[AT][AT]GG
FokI 666 CATCC GGATG(9/13)
HaeIII 3 GGCC GG^CC
HincII 514 GTCAAC GT[CT]^[GA]AC
HindII 514 GTCAAC GT[CT]^[GA]AC
HphI 538 TCACC GGTGA(8/7)
MflI 674 AGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 683 CAGCTG C[AC]G^C[GT]G
NlaIV 502 GGCTCC GG[ACGT]^[ACGT]CC
PvuII 683 CAGCTG CAG^CTG
RcaI 398 TCATGA T^CATGA
RsaI 171 GTAC GT^AC
SfaNI 234 GATGC GCATC(5/9)
SmlI 108 CTTGAG C^T[CT][GA]AG
StyI 51 CCTTGG C^C[AT][AT]GG
XhoII 674 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 675 GATCC GGATC(4/5)
147 GGATC
BbvI 63 GCAGC GCAGC(8/12)
364 GCTGC
BfaI 47 CTAG C^TAG
181 CTAG
BsaBI 234 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
627 GATATTTATC
BsaJI 51 CCTTGG C^C[ACGT][ACGT]GG
306 CCTCGG
BsrI 582 ACTGG ACTGG(1/-1)
541 CCAGT
DpnII 148 GATC ^GATC
675 GATC
Fnu4HI 63 GCAGC GC^[ACGT]GC
364 GCTGC
ItaI 63 GCAGC GC^[ACGT]GC
364 GCTGC
MaeI 47 CTAG C^TAG
181 CTAG
MaeIII 19 GTCAC ^GT[ACGT]AC
319 GTCAC
MamI 234 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
627 GATATTTATC
MboI 148 GATC ^GATC
675 GATC
MslI 162 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
288 CACCCAAATG
NdeII 148 GATC ^GATC
675 GATC
NlaIII 168 CATG CATG^
399 CATG
Sau3AI 148 GATC ^GATC
675 GATC
TseI 63 GCAGC G^C[AT]GC
364 GCTGC
Tsp45I 19 GTCAC ^GT[GC]AC
319 GTCAC
Tsp509I 7 AATT ^AATT
59 AATT
TspRI 21 CACTG CA[GC]TG[ACGT][ACGT]^
576 CAGTG
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
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AluI DdeI MboII MnlI MwoI