##################################
# NO CUT ENZYMES (  173 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BcnI        
BfrI        BglI        BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoT22I     FauI        Fnu4HI      
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       ItaI        KasI        Ksp632I     
KspI        MamI        MfeI        MluI        MroI        
MseI        MspA1I      MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        Tru9I       TseI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        365  GGTACC               G^GTACC
Asp718I       365  GGTACC               G^GTACC
BanI          365  GGTACC               G^G[CT][GA]CC
BanII         606  GAGCTC               G[GA]GC[CT]^C
BclI          522  TGATCA               T^GATCA
BglII         209  AGATCT               A^GATCT
BsaAI         178  TACGTG               [CT]AC^GT[GA]
BsmAI         435  GTCTC                GTCTC(1/5)
BsmI          331  GAATGC               GAATGC(1/-1)
Bsp1407I      635  TGTACA               T^GTACA
BspMI         223  ACCTGC               ACCTGC(4/8)
BsrGI         635  TGTACA               T^GTACA
BstYI         209  AGATCT               [GA]^GATC[CT]
Cac8I         413  GCCAGC               GC[ACGT]^[ACGT]GC
DraII         646  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          629  CCATGG               C^C[GA][CT]GG
EcoO109I      646  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV          15  GATATC               GAT^ATC
FbaI          522  TGATCA               T^GATCA
HgaI           27  GACGC                GACGC(5/10)
HinfI         161  GACTC                G^A[ACGT]TC
HphI          626  TCACC                GGTGA(8/7)
KpnI          365  GGTACC               GGTAC^C
MaeII         179  ACGT                 A^CGT
MflI          209  AGATCT               [GA]^GATC[CT]
NcoI          629  CCATGG               C^CATGG
PleI          161  GACTC                GAGTC(4/5)
PpuMI         646  GGGTCCT              [GA]G^G[AT]CC[CT]
SacI          606  GAGCTC               GAGCT^C
SfaNI         425  GCATC                GCATC(5/9)
SspBI         635  TGTACA               T^GTACA
TaqI          159  TCGA                 T^CGA
TthHB8I       159  TCGA                 T^CGA
XhoII         209  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AccI          100  GTCTAC               GT^[AC][GT]AC
              541  GTAGAC              
AcsI           65  AAATTC               [GA]^AATT[CT]
              249  AAATTT              
AluI          416  AGCT                 AG^CT
              607  AGCT                
ApoI           65  AAATTC               [GA]^AATT[CT]
              249  AAATTT              
BfaI          187  CTAG                 C^TAG
              205  CTAG                
BsrI          118  ACTGG                ACTGG(1/-1)
              409  ACTGG               
BstF5I        639  CATCC                GGATG(2/0)
              286  GGATG               
BstXI         236  CCAACATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              414  CCAGCTCTGTGG        
FokI          639  CATCC                GGATG(9/13)
              286  GGATG               
MaeI          187  CTAG                 C^TAG
              205  CTAG                
MaeIII         25  GTGAC                ^GT[ACGT]AC
              220  GTGAC               
MslI          426  CATCTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              627  CACCATGGTG          
NspI          239  ACATGC               [GA]CATG^[CT]
              588  GCATGT              
Tsp45I         25  GTGAC                ^GT[GC]AC
              220  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AfaI        AspHI       AvaII       BalI        BmyI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmFI       Bsp1286I    BssKI       BstNI       Cfr13I      
DdeI        DpnII       EaeI        EcoRII      EcoT14I     
HaeIII      MboI        MboII       MluNI       MnlI        
MscI        MvaI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       StyI        
Tsp509I     TspRI