Restriction map for
pF1KE0814
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI HpaII
KasI KpnI KspI MaeI MaeII
MamI MluI MroI MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AocI 756 CCTGAGG CC^T[ACGT]AGG
AseI 33 ATTAAT AT^TAAT
AsnI 33 ATTAAT AT^TAAT
Asp700I 31 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 508 GTGCTC G[AT]GC[AT]^C
AvaII 156 GGTCC G^G[AT]CC
BamHI 761 GGATCC G^GATCC
BbvCI 267 CCTCAGC CCTCAGC(-5/-2)
BglI 427 GCCACAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 508 GTGCTC G[AGT]GC[ACT]^C
BsgI 376 GTGCAG GTGCAG(16/14)
BsiEI 441 CGACCG CG[GA][CT]^CG
BsiHKAI 508 GTGCTC G[AT]GC[AT]^C
BsiYI 444 CCGTTACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 444 CCGTTACATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 508 GTGCTC G[AGT]GC[ACT]^C
BsrBI 343 GAGCGG CCGCTC(-3/-3)
BssKI 195 CCTGG ^CC[ACGT]GG
BstNI 195 CCTGG CC^[AT]GG
BstXI 188 CCATTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 761 GGATCC [GA]^GATC[CT]
Bsu36I 756 CCTGAGG CC^T[ACGT]AGG
DsaI 80 CCATGG C^C[GA][CT]GG
EarI 160 CTCTTC CTCTTC(1/4)
Eco81I 756 CCTGAGG CC^T[ACGT]AGG
EcoRII 195 CCTGG ^CC[AT]GG
EcoT14I 80 CCATGG C^C[AT][AT]GG
FauI 345 GCGGG CCCGC(4/6)
Ksp632I 160 CTCTTC CTCTTC(1/4)
MfeI 560 CAATTG C^AATTG
MflI 761 GGATCC [GA]^GATC[CT]
MslI 249 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 270 CAGCTG C[AC]G^C[GT]G
MunI 560 CAATTG C^AATTG
MvaI 195 CCTGG CC^[AT]GG
MwoI 427 GCCACAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 80 CCATGG C^CATGG
NlaIV 761 GGATCC GG[ACGT]^[ACGT]CC
NspI 367 GCATGT [GA]CATG^[CT]
PshBI 33 ATTAAT AT^TAAT
PvuII 270 CAGCTG CAG^CTG
ScrFI 195 CCTGG CC^[ACGT]GG
StyI 80 CCATGG C^C[AT][AT]GG
Tsp45I 605 GTCAC ^GT[GC]AC
XcmI 188 CCATTTCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 761 GGATCC [GA]^GATC[CT]
XmnI 31 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 762 GATCC GGATC(4/5)
761 GGATC
BalI 425 TGGCCA TGG^CCA
452 TGGCCA
BbvI 324 GCTGC GCAGC(8/12)
748 GCTGC
BsaJI 80 CCATGG C^C[ACGT][ACGT]GG
194 CCCTGG
BsmI 212 GAATGC GAATGC(1/-1)
320 GAATGC
Cfr13I 156 GGTCC G^G[ACGT]CC
589 GGGCC
EaeI 425 TGGCCA [CT]^GGCC[GA]
452 TGGCCA
Fnu4HI 324 GCTGC GC^[ACGT]GC
748 GCTGC
HphI 570 GGTGA GGTGA(8/7)
723 GGTGA
ItaI 324 GCTGC GC^[ACGT]GC
748 GCTGC
MluNI 425 TGGCCA TGG^CCA
452 TGGCCA
MscI 425 TGGCCA TGG^CCA
452 TGGCCA
Sau96I 156 GGTCC G^G[ACGT]CC
589 GGGCC
SfaNI 289 GATGC GCATC(5/9)
62 GCATC
TseI 324 GCTGC G^C[AT]GC
748 GCTGC
TspRI 408 CACTG CA[GC]TG[ACGT][ACGT]^
629 CACTG
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
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AciI AluI BsmAI Cac8I DdeI
DpnII HaeIII MaeIII MboI MboII
MnlI MseI NdeII NlaIII Sau3AI
Tru9I Tsp509I