Restriction map for
pF1KE0808
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BcnI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstPI BstUI BstYI
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MflI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 385 GTATAC GT^[AC][GT]AC
AlwI 558 GATCC GGATC(4/5)
AocI 136 CCTGAGG CC^T[ACGT]AGG
AspHI 436 GAGCAC G[AT]GC[AT]^C
BalI 272 TGGCCA TGG^CCA
BanII 70 GAGCCC G[GA]GC[CT]^C
BbvCI 101 CCTCAGC CCTCAGC(-5/-2)
BclI 44 TGATCA T^GATCA
BfaI 724 CTAG C^TAG
BglI 451 GCCAGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 615 TACGTG [CT]AC^GT[GA]
BsiHKAI 436 GAGCAC G[AT]GC[AT]^C
BsmI 661 GCATTC GAATGC(1/-1)
Bsp1407I 2 TGTACA T^GTACA
BspHI 47 TCATGA T^CATGA
BsrGI 2 TGTACA T^GTACA
BsrI 522 ACTGG ACTGG(1/-1)
Bst1107I 385 GTATAC GTA^TAC
BstAPI 229 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 681 CCATCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 385 GTATAC GTA^TAC
Bsu36I 136 CCTGAGG CC^T[ACGT]AGG
CfoI 57 GCGC GCG^C
DraII 605 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 565 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 167 CCATGG C^C[GA][CT]GG
EaeI 272 TGGCCA [CT]^GGCC[GA]
EarI 82 CTCTTC CTCTTC(1/4)
Eco81I 136 CCTGAGG CC^T[ACGT]AGG
EcoO109I 605 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 167 CCATGG C^C[AT][AT]GG
FbaI 44 TGATCA T^GATCA
HhaI 57 GCGC GCG^C
HinP1I 57 GCGC G^CGC
HinfI 232 GAATC G^A[ACGT]TC
Ksp632I 82 CTCTTC CTCTTC(1/4)
MaeI 724 CTAG C^TAG
MaeII 616 ACGT A^CGT
MaeIII 281 GTTAC ^GT[ACGT]AC
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
NcoI 167 CCATGG C^CATGG
NlaIV 158 GGTTCC GG[ACGT]^[ACGT]CC
PflMI 162 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 128 CTGCAG CTGCA^G
PvuII 338 CAGCTG CAG^CTG
RcaI 47 TCATGA T^CATGA
SspBI 2 TGTACA T^GTACA
StyI 167 CCATGG C^C[AT][AT]GG
TaqI 804 TCGA T^CGA
TfiI 232 GAATC G^A[AT]TC
Tsp509I 795 AATT ^AATT
TthHB8I 804 TCGA T^CGA
Van91I 162 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 3 GTAC GT^AC
614 GTAC
AluI 339 AGCT AG^CT
352 AGCT
AlwNI 131 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
230 CAGAATCTG
BbsI 783 GAAGAC GAAGAC(2/6)
582 GTCTTC
BpuAI 783 GAAGAC GAAGAC(2/6)
582 GTCTTC
BsaJI 167 CCATGG C^C[ACGT][ACGT]GG
587 CCTGGG
BsrDI 447 CATTGC GCAATG(2/0)
250 GCAATG
BstF5I 702 CATCC GGATG(2/0)
720 CATCC
Cfr13I 504 GGCCC G^G[ACGT]CC
606 GGCCC
FokI 702 CATCC GGATG(9/13)
720 CATCC
HphI 780 GGTGA GGTGA(8/7)
708 TCACC
MseI 304 TTAA T^TAA
797 TTAA
MspA1I 122 CCGCTG C[AC]G^C[GT]G
338 CAGCTG
RsaI 3 GTAC GT^AC
614 GTAC
Sau96I 504 GGCCC G^G[ACGT]CC
606 GGCCC
SfcI 128 CTGCAG C^T[GA][CT]AG
515 CTACAG
Tru9I 304 TTAA T^TAA
797 TTAA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI BbvI BmyI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI Cac8I
DdeI DpnII EcoRII Fnu4HI HaeIII
ItaI MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII Sau3AI
ScrFI TseI TspRI