Restriction map for
pF1KE0803
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI DsaI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MaeIII MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Alw44I 778 GTGCAC G^TGCAC
AlwI 204 GGATC GGATC(4/5)
AlwNI 361 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 864 AGCGCT AGC^GCT
ApaI 443 GGGCCC GGGCC^C
ApaLI 778 GTGCAC G^TGCAC
AspHI 778 GTGCAC G[AT]GC[AT]^C
AvaII 852 GGTCC G^G[AT]CC
BalI 233 TGGCCA TGG^CCA
BbrPI 28 CACGTG CAC^GTG
BglI 840 GCCCCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 856 CTCCAG CTGGAG(16/14)
BsaAI 28 CACGTG [CT]AC^GT[GA]
BsaI 901 GAGACC GGTCTC(1/5)
BsiEI 344 CGGCCG CG[GA][CT]^CG
BsiHKAI 778 GTGCAC G[AT]GC[AT]^C
BsmBI 872 GAGACG CGTCTC(1/5)
BsrFI 260 GCCGGC [GA]^CCGG[CT]
BssSI 393 CTCGTG CACGAG(-5/-1)
BstAPI 462 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 204 GGATCT [GA]^GATC[CT]
Cfr10I 260 GCCGGC [GA]^CCGG[CT]
DpnII 205 GATC ^GATC
EagI 344 CGGCCG C^GGCCG
EclXI 344 CGGCCG C^GGCCG
Eco47III 864 AGCGCT AGC^GCT
Eco52I 344 CGGCCG C^GGCCG
EcoNI 87 CCTCACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 216 CCTTGG C^C[AT][AT]GG
HgaI 525 GACGC GACGC(5/10)
HinfI 333 GAGTC G^A[ACGT]TC
MboI 205 GATC ^GATC
MflI 204 GGATCT [GA]^GATC[CT]
MluNI 233 TGGCCA TGG^CCA
MscI 233 TGGCCA TGG^CCA
MslI 420 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 260 GCCGGC GCC^GGC
NdeII 205 GATC ^GATC
NgoMIV 260 GCCGGC G^CCGGC
PflMI 225 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 333 GAGTC GAGTC(4/5)
PmaCI 28 CACGTG CAC^GTG
PmlI 28 CACGTG CAC^GTG
Sau3AI 205 GATC ^GATC
SfaNI 701 GCATC GCATC(5/9)
SfcI 159 CTACAG C^T[GA][CT]AG
StyI 216 CCTTGG C^C[AT][AT]GG
Van91I 225 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 176 CCATCTACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 204 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 157 GTCTAC GT^[AC][GT]AC
802 GTCTAC
AccII 201 CGCG CG^CG
786 CGCG
AcyI 46 GGCGCC G[GA]^CG[CT]C
478 GGCGCC
AfaI 390 GTAC GT^AC
732 GTAC
AvaI 273 CCCGGG C^[CT]CG[GA]G
635 CCCGGG
BanI 46 GGCGCC G^G[CT][GA]CC
478 GGCGCC
BbeI 46 GGCGCC GGCGC^C
478 GGCGCC
BsaHI 46 GGCGCC G[GA]^CG[CT]C
478 GGCGCC
BseRI 658 CTCCTC GAGGAG(10/8)
670 CTCCTC
BsoBI 273 CCCGGG C^[CT]CG[GA]G
635 CCCGGG
BspMI 464 ACCTGC ACCTGC(4/8)
722 ACCTGC
BsrI 707 ACTGG ACTGG(1/-1)
489 CCAGT
BstUI 201 CGCG CG^CG
786 CGCG
DraIII 226 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
570 CACCATGTG
FauI 908 CCCGC CCCGC(4/6)
603 GCGGG
Hin1I 46 GGCGCC G[GA]^CG[CT]C
478 GGCGCC
KasI 46 GGCGCC G^GCGCC
478 GGCGCC
MaeII 29 ACGT A^CGT
191 ACGT
MvnI 201 CGCG CG^CG
786 CGCG
NarI 46 GGCGCC GG^CGCC
478 GGCGCC
RsaI 390 GTAC GT^AC
732 GTAC
SfoI 46 GGCGCC GGC^GCC
478 GGCGCC
SmaI 273 CCCGGG CCC^GGG
635 CCCGGG
XmaCI 273 CCCGGG C^CCGGG
635 CCCGGG
XmaI 273 CCCGGG C^CCGGG
635 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BlpI BmyI Bpu1102I BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I CelII
CfoI Cfr13I DdeI DraII EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
TseI TspRI