Restriction map for
pF1KE0791
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BbvI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsmBI
BsmFI Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRII EcoRV
EcoT22I FauI Fnu4HI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaI HpaII ItaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvaI MvnI MwoI
NaeI NarI NciI NcoI NgoMIV
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TseI Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 140 GCGG CCGC(-3/-1)
AfaI 628 GTAC GT^AC
AflIII 822 ACATGT A^C[GA][CT]GT
AseI 1050 ATTAAT AT^TAAT
AsnI 1050 ATTAAT AT^TAAT
Asp700I 723 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 832 GAGCTC G[AT]GC[AT]^C
AvaI 408 CCCGAG C^[CT]CG[GA]G
AvaII 620 GGACC G^G[AT]CC
BanII 832 GAGCTC G[GA]GC[CT]^C
BglII 314 AGATCT A^GATCT
BmyI 832 GAGCTC G[AGT]GC[ACT]^C
BseRI 547 GAGGAG GAGGAG(10/8)
BsiHKAI 832 GAGCTC G[AT]GC[AT]^C
BsmI 788 GCATTC GAATGC(1/-1)
BsoBI 408 CCCGAG C^[CT]CG[GA]G
Bsp1286I 832 GAGCTC G[AGT]GC[ACT]^C
BspHI 1043 TCATGA T^CATGA
BspLU11I 822 ACATGT A^CATGT
Cfr13I 620 GGACC G^G[ACGT]CC
DraI 1030 TTTAAA TTT^AAA
DraII 619 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 452 CCGTGG C^C[GA][CT]GG
EaeI 95 TGGCCG [CT]^GGCC[GA]
EarI 108 GAAGAG CTCTTC(1/4)
EcoO109I 619 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 176 CCAAGG C^C[AT][AT]GG
HaeIII 96 GGCC GG^CC
HincII 70 GTTGAC GT[CT]^[GA]AC
HindII 70 GTTGAC GT[CT]^[GA]AC
Ksp632I 108 GAAGAG CTCTTC(1/4)
NdeI 188 CATATG CA^TATG
PpuMI 619 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1050 ATTAAT AT^TAAT
RcaI 1043 TCATGA T^CATGA
RsaI 628 GTAC GT^AC
SacI 832 GAGCTC GAGCT^C
SapI 108 GAAGAGC GCTCTTC(1/4)
Sau96I 620 GGACC G^G[ACGT]CC
SfcI 586 CTACAG C^T[GA][CT]AG
SspI 195 AATATT AAT^ATT
StyI 176 CCAAGG C^C[AT][AT]GG
TaqI 952 TCGA T^CGA
TthHB8I 952 TCGA T^CGA
XbaI 372 TCTAGA T^CTAGA
XmnI 723 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 534 AAATTT [GA]^AATT[CT]
727 AAATTC
ApoI 534 AAATTT [GA]^AATT[CT]
727 AAATTC
BbsI 258 GAAGAC GAAGAC(2/6)
62 GTCTTC
BclI 84 TGATCA T^GATCA
923 TGATCA
BpuAI 258 GAAGAC GAAGAC(2/6)
62 GTCTTC
BsaJI 176 CCAAGG C^C[ACGT][ACGT]GG
452 CCGTGG
BstF5I 336 CATCC GGATG(2/0)
756 GGATG
Cac8I 249 GCTAGC GC[ACGT]^[ACGT]GC
498 GCTAGC
FbaI 84 TGATCA T^GATCA
923 TGATCA
FokI 336 CATCC GGATG(9/13)
756 GGATG
HindIII 417 AAGCTT A^AGCTT
868 AAGCTT
MslI 301 CATTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
694 CACAAGTGTG
NheI 249 GCTAGC G^CTAGC
498 GCTAGC
PleI 293 GAGTC GAGTC(4/5)
741 GAGTC
SfaNI 4 GCATC GCATC(5/9)
610 GCATC
Tsp45I 308 GTGAC ^GT[GC]AC
849 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwI BfaI BsmAI BsrI
BstYI DdeI DpnII HinfI HphI
MaeI MaeIII MboI MboII MflI
MnlI MseI NdeII NlaIII NspI
Sau3AI TfiI Tru9I Tsp509I TspRI
XhoII