Restriction map for
pF1KE0790
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I FauI FbaI FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MroI MunI
MvnI MwoI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AocI 58 CCTCAGG CC^T[ACGT]AGG
AspI 1216 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 123 GGACC G^G[AT]CC
BalI 86 TGGCCA TGG^CCA
BanII 241 GAGCCC G[GA]GC[CT]^C
BbvI 408 GCAGC GCAGC(8/12)
BcnI 1153 CCGGG CC^[GC]GG
BfaI 506 CTAG C^TAG
BmyI 241 GAGCCC G[AGT]GC[ACT]^C
BsaI 1275 GAGACC GGTCTC(1/5)
BsiYI 20 CCCAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 20 CCCAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1275 GAGAC GTCTC(1/5)
Bsp1286I 241 GAGCCC G[AGT]GC[ACT]^C
BsrDI 341 GCAATG GCAATG(2/0)
BstF5I 47 GGATG GGATG(2/0)
Bsu36I 58 CCTCAGG CC^T[ACGT]AGG
Cac8I 649 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 1230 GCGC GCG^C
DpnII 544 GATC ^GATC
EaeI 86 TGGCCA [CT]^GGCC[GA]
Eco81I 58 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1292 ATGCAT ATGCA^T
Fnu4HI 408 GCAGC GC^[ACGT]GC
FokI 47 GGATG GGATG(9/13)
HhaI 1230 GCGC GCG^C
HinP1I 1230 GCGC G^CGC
HindIII 884 AAGCTT A^AGCTT
ItaI 408 GCAGC GC^[ACGT]GC
MaeI 506 CTAG C^TAG
MboI 544 GATC ^GATC
MluNI 86 TGGCCA TGG^CCA
MscI 86 TGGCCA TGG^CCA
MslI 78 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 409 CAGCTG C[AC]G^C[GT]G
NciI 1153 CCGGG CC^[GC]GG
NdeII 544 GATC ^GATC
NsiI 1292 ATGCAT ATGCA^T
NspI 1294 GCATGT [GA]CATG^[CT]
PflFI 1216 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 409 CAGCTG CAG^CTG
Sau3AI 544 GATC ^GATC
SexAI 176 ACCTGGT A^CC[AT]GGT
SfaNI 1083 GCATC GCATC(5/9)
SfcI 168 CTACAG C^T[GA][CT]AG
SmlI 385 CTTGAG C^T[CT][GA]AG
TaqI 92 TCGA T^CGA
TseI 408 GCAGC G^C[AT]GC
TspRI 1364 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1216 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 92 TCGA T^CGA
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 680 CCGC CCGC(-3/-1)
1018 GCGG
BsaJI 119 CCCTGG C^C[ACGT][ACGT]GG
126 CCCTGG
BseRI 106 GAGGAG GAGGAG(10/8)
321 GAGGAG
HapII 422 CCGG C^CGG
1153 CCGG
HgaI 65 GACGC GACGC(5/10)
1149 GCGTC
HpaII 422 CCGG C^CGG
1153 CCGG
HphI 628 GGTGA GGTGA(8/7)
484 TCACC
MaeII 83 ACGT A^CGT
152 ACGT
MspI 422 CCGG C^CGG
1153 CCGG
NlaIV 123 GGACCC GG[ACGT]^[ACGT]CC
173 GGAACC
SapI 1133 GAAGAGC GCTCTTC(1/4)
52 GCTCTTC
TfiI 191 GAATC G^A[AT]TC
249 GATTC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BpmI BpuAI BsmFI BsrI BssKI
BstNI Cfr13I DdeI EarI EcoRI
EcoRII HaeIII HinfI Ksp632I MaeIII
MboII MnlI MseI MvaI NlaIII
PleI RsaI Sau96I ScrFI Tru9I
Tsp45I Tsp509I