Restriction map for
pF1KE0781
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI AluI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I FbaI FseI FspI HaeII
HapII Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 584 ACATGT A^C[GA][CT]GT
Alw44I 967 GTGCAC G^TGCAC
ApaLI 967 GTGCAC G^TGCAC
BanI 379 GGTGCC G^G[CT][GA]CC
BbsI 808 GAAGAC GAAGAC(2/6)
BciVI 496 GTATCC GTATCC(6/5)
BglI 521 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 808 GAAGAC GAAGAC(2/6)
BsaAI 57 TACGTG [CT]AC^GT[GA]
BseRI 259 GAGGAG GAGGAG(10/8)
BsmAI 581 GAGAC GTCTC(1/5)
BspHI 533 TCATGA T^CATGA
BspLU11I 584 ACATGT A^CATGT
BspMI 821 ACCTGC ACCTGC(4/8)
BstXI 1022 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 852 AGATCC [GA]^GATC[CT]
CfoI 1002 GCGC GCG^C
DdeI 375 CTTAG C^T[ACGT]AG
DraIII 1005 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 283 GATATC GAT^ATC
EcoT14I 995 CCAAGG C^C[AT][AT]GG
EcoT22I 303 ATGCAT ATGCA^T
FauI 144 CCCGC CCCGC(4/6)
HgaI 479 GCGTC GACGC(5/10)
HhaI 1002 GCGC GCG^C
HinP1I 1002 GCGC G^CGC
HphI 888 GGTGA GGTGA(8/7)
MaeII 58 ACGT A^CGT
MflI 852 AGATCC [GA]^GATC[CT]
MspA1I 145 CCGCTG C[AC]G^C[GT]G
MwoI 521 GCCAGGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 303 ATGCAT ATGCA^T
NspI 584 ACATGT [GA]CATG^[CT]
PflMI 658 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 568 CTGCAG CTGCA^G
RcaI 533 TCATGA T^CATGA
SfcI 568 CTGCAG C^T[GA][CT]AG
StuI 528 AGGCCT AGG^CCT
StyI 995 CCAAGG C^C[AT][AT]GG
TaqI 354 TCGA T^CGA
TthHB8I 354 TCGA T^CGA
Van91I 658 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 432 TCTAGA T^CTAGA
XcmI 590 CCAAGAGTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 852 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 145 CCGC CCGC(-3/-1)
12 GCGG
AspHI 156 GAGCAC G[AT]GC[AT]^C
967 GTGCAC
AvaII 231 GGACC G^G[AT]CC
347 GGTCC
BfaI 167 CTAG C^TAG
433 CTAG
BmyI 156 GAGCAC G[AGT]GC[ACT]^C
967 GTGCAC
BpmI 256 CTGGAG CTGGAG(16/14)
578 CTGGAG
BsgI 823 CTGCAC GTGCAG(16/14)
596 GTGCAG
BsiHKAI 156 GAGCAC G[AT]GC[AT]^C
967 GTGCAC
Bsp1286I 156 GAGCAC G[AGT]GC[ACT]^C
967 GTGCAC
Cac8I 573 GCCTGC GC[ACGT]^[ACGT]GC
981 GCAAGC
Cfr13I 231 GGACC G^G[ACGT]CC
347 GGTCC
MaeI 167 CTAG C^TAG
433 CTAG
MslI 826 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1023 CATCCAGATG
NlaIV 3 GGAACC GG[ACGT]^[ACGT]CC
379 GGTGCC
Sau96I 231 GGACC G^G[ACGT]CC
347 GGTCC
SfaNI 268 GATGC GCATC(5/9)
640 GATGC
SmlI 801 CTTGAG C^T[CT][GA]AG
873 CTTGAG
Tsp45I 119 GTGAC ^GT[GC]AC
889 GTGAC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AlwI ApoI BbvI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI DpnII EarI EcoRI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII Sau3AI
ScrFI TfiI Tru9I TseI Tsp509I
TspRI