Restriction map for
pF1KE0417
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tru9I
Tsp45I Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 204 GTAC GT^AC
AhdI 667 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 118 GATCC GGATC(4/5)
AlwNI 461 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 432 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 667 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 480 GAGCTC G[AT]GC[AT]^C
AvaI 751 CTCGAG C^[CT]CG[GA]G
AvaII 519 GGACC G^G[AT]CC
BcgI 560 GCAAGTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 61 CTAG C^TAG
BsaI 256 GAGACC GGTCTC(1/5)
BsiHKAI 480 GAGCTC G[AT]GC[AT]^C
BsoBI 751 CTCGAG C^[CT]CG[GA]G
BstBI 104 TTCGAA TT^CGAA
BstEII 19 GGTAACC G^GT[ACGT]ACC
BstPI 19 GGTAACC G^GT[ACGT]ACC
BstYI 117 AGATCC [GA]^GATC[CT]
CfoI 72 GCGC GCG^C
DraII 755 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 667 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 311 GAAGAG CTCTTC(1/4)
EcoO109I 755 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 19 GGTAACC G^GT[ACGT]ACC
HhaI 72 GCGC GCG^C
HinP1I 72 GCGC G^CGC
Ksp632I 311 GAAGAG CTCTTC(1/4)
MaeI 61 CTAG C^TAG
MaeIII 20 GTAAC ^GT[ACGT]AC
MflI 117 AGATCC [GA]^GATC[CT]
MwoI 680 GCTCAGACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 231 CATG CATG^
NspV 104 TTCGAA TT^CGAA
PaeR7I 751 CTCGAG C^TCGAG
PstI 614 CTGCAG CTGCA^G
RsaI 204 GTAC GT^AC
SacI 480 GAGCTC GAGCT^C
SfaNI 629 GCATC GCATC(5/9)
SfuI 104 TTCGAA TT^CGAA
StuI 639 AGGCCT AGG^CCT
TfiI 540 GAATC G^A[AT]TC
XhoI 751 CTCGAG C^TCGAG
XhoII 117 AGATCC [GA]^GATC[CT]
XmnI 432 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 220 AAATTC [GA]^AATT[CT]
368 GAATTT
ApoI 220 AAATTC [GA]^AATT[CT]
368 GAATTT
BanI 646 GGTGCC G^G[CT][GA]CC
694 GGCACC
BanII 480 GAGCTC G[GA]GC[CT]^C
547 GAGCCC
BmyI 480 GAGCTC G[AGT]GC[ACT]^C
547 GAGCCC
BsaWI 516 ACCGGA [AT]^CCGG[AT]
709 ACCGGA
BseRI 723 CTCCTC GAGGAG(10/8)
136 GAGGAG
Bsp1286I 480 GAGCTC G[AGT]GC[ACT]^C
547 GAGCCC
DpnII 118 GATC ^GATC
155 GATC
EcoNI 267 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
354 CCTCAAGAAGG
HapII 517 CCGG C^CGG
710 CCGG
HpaII 517 CCGG C^CGG
710 CCGG
MboI 118 GATC ^GATC
155 GATC
MspI 517 CCGG C^CGG
710 CCGG
NdeII 118 GATC ^GATC
155 GATC
PleI 667 GACTC GAGTC(4/5)
673 GAGTC
Sau3AI 118 GATC ^GATC
155 GATC
Tsp509I 221 AATT ^AATT
369 AATT
TspRI 498 CAGTG CA[GC]TG[ACGT][ACGT]^
601 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsmAI BssKI BstF5I BstNI Cfr13I
DdeI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MboII MnlI MvaI
NlaIV Sau96I ScrFI SfcI SmlI
TaqI TseI TthHB8I