Restriction map for
pF1KE0415
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstF5I
BstPI BstUI Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I FauI FbaI FokI FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I Tth111I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 379 GTATAC GT^[AC][GT]AC
AciI 450 CCGC CCGC(-3/-1)
AfaI 88 GTAC GT^AC
AluI 126 AGCT AG^CT
AlwI 316 GGATC GGATC(4/5)
AseI 208 ATTAAT AT^TAAT
AsnI 208 ATTAAT AT^TAAT
AspHI 275 GTGCTC G[AT]GC[AT]^C
BbsI 457 GAAGAC GAAGAC(2/6)
BfaI 13 CTAG C^TAG
BmyI 275 GTGCTC G[AGT]GC[ACT]^C
BpuAI 457 GAAGAC GAAGAC(2/6)
BsiHKAI 275 GTGCTC G[AT]GC[AT]^C
Bsp1286I 275 GTGCTC G[AGT]GC[ACT]^C
BsrDI 75 CATTGC GCAATG(2/0)
BsrI 44 ACTGG ACTGG(1/-1)
BssKI 385 CCTGG ^CC[ACGT]GG
Bst1107I 379 GTATAC GTA^TAC
BstNI 385 CCTGG CC^[AT]GG
BstXI 168 CCATGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 316 GGATCT [GA]^GATC[CT]
BstZ17I 379 GTATAC GTA^TAC
Cac8I 277 GCTCGC GC[ACGT]^[ACGT]GC
CfoI 57 GCGC GCG^C
DdeI 30 CTCAG C^T[ACGT]AG
DpnII 317 GATC ^GATC
EaeI 447 TGGCCG [CT]^GGCC[GA]
EcoRII 385 CCTGG ^CC[AT]GG
EcoT22I 36 ATGCAT ATGCA^T
HhaI 57 GCGC GCG^C
HinP1I 57 GCGC G^CGC
HinfI 339 GATTC G^A[ACGT]TC
HphI 238 GGTGA GGTGA(8/7)
MaeI 13 CTAG C^TAG
MaeIII 245 GTTAC ^GT[ACGT]AC
MboI 317 GATC ^GATC
MboII 457 GAAGA GAAGA(8/7)
MflI 316 GGATCT [GA]^GATC[CT]
MspA1I 125 CAGCTG C[AC]G^C[GT]G
MvaI 385 CCTGG CC^[AT]GG
NdeII 317 GATC ^GATC
NsiI 36 ATGCAT ATGCA^T
PshBI 208 ATTAAT AT^TAAT
PvuII 125 CAGCTG CAG^CTG
RsaI 88 GTAC GT^AC
Sau3AI 317 GATC ^GATC
ScrFI 385 CCTGG CC^[ACGT]GG
SmlI 21 CTTGAG C^T[CT][GA]AG
TaqI 220 TCGA T^CGA
TfiI 339 GATTC G^A[AT]TC
Tsp509I 390 AATT ^AATT
TthHB8I 220 TCGA T^CGA
XbaI 12 TCTAGA T^CTAGA
XhoII 316 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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Asp700I 147 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
408 GAATGCCTTC
BbvI 124 GCAGC GCAGC(8/12)
79 GCTGC
BsaJI 234 CCGAGG C^C[ACGT][ACGT]GG
384 CCCTGG
BsmI 34 GAATGC GAATGC(1/-1)
408 GAATGC
HaeIII 309 GGCC GG^CC
448 GGCC
MseI 209 TTAA T^TAA
440 TTAA
MslI 169 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
329 CATCTATGTG
Tru9I 209 TTAA T^TAA
440 TTAA
TseI 79 GCTGC G^C[AT]GC
124 GCAGC
TspRI 249 CAGTG CA[GC]TG[ACGT][ACGT]^
453 CACTG
XmnI 147 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
408 GAATGCCTTC
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
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Fnu4HI ItaI MnlI MwoI NlaIII
SfaNI