Restriction map for
pF1KE0393
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AvrII BciVI BfrI
BlnI BlpI Bpu1102I BsaBI BsiWI
Bsp1407I BspDI BspHI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
ClaI DraI DraIII Eco47III EcoRI
EcoRV EcoT22I HindIII HpaI KpnI
MamI MfeI MluI MseI MunI
NdeI NheI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 2722 GACGTC GACGT^C
AccIII 1482 TCCGGA T^CCGGA
AhdI 53 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 406 GTGCAC G^TGCAC
AocI 2395 CCTGAGG CC^T[ACGT]AGG
ApaLI 406 GTGCAC G^TGCAC
AspEI 53 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2002 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 278 TGCGCA TGC^GCA
BalI 2837 TGGCCA TGG^CCA
BamHI 2763 GGATCC G^GATCC
BbeI 1779 GGCGCC GGCGC^C
BbrPI 1072 CACGTG CAC^GTG
BcgI 285 CGAGCGTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1019 TGATCA T^GATCA
BfaI 1198 CTAG C^TAG
BglII 2276 AGATCT A^GATCT
BsaWI 1482 TCCGGA [AT]^CCGG[AT]
BseAI 1482 TCCGGA T^CCGGA
BsmI 2328 GAATGC GAATGC(1/-1)
BspEI 1482 TCCGGA T^CCGGA
BsrBI 1524 GAGCGG CCGCTC(-3/-3)
Bsu36I 2395 CCTGAGG CC^T[ACGT]AGG
CpoI 2081 CGGTCCG CG^G[AT]CCG
DrdI 1276 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 53 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1879 CTCTTC CTCTTC(1/4)
Eco81I 2395 CCTGAGG CC^T[ACGT]AGG
EcoNI 1878 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1019 TGATCA T^GATCA
FseI 1657 GGCCGGCC GGCCGG^CC
FspI 278 TGCGCA TGC^GCA
KasI 1779 GGCGCC G^GCGCC
Ksp632I 1879 CTCTTC CTCTTC(1/4)
KspI 1405 CCGCGG CCGC^GG
MaeI 1198 CTAG C^TAG
MluNI 2837 TGGCCA TGG^CCA
MroI 1482 TCCGGA T^CCGGA
MscI 2837 TGGCCA TGG^CCA
NarI 1779 GGCGCC GG^CGCC
NruI 1818 TCGCGA TCG^CGA
PflFI 2002 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1647 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1072 CACGTG CAC^GTG
PmlI 1072 CACGTG CAC^GTG
PshAI 585 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2081 CGGTCCG CG^G[AT]CCG
SacI 1201 GAGCTC GAGCT^C
SacII 1405 CCGCGG CCGC^GG
SalI 2008 GTCGAC G^TCGAC
SfoI 1779 GGCGCC GGC^GCC
SphI 90 GCATGC GCATG^C
StuI 2428 AGGCCT AGG^CCT
Tth111I 2002 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1647 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 2656 GGGCCC GGGCC^C
2830 GGGCCC
BbsI 450 GAAGAC GAAGAC(2/6)
957 GAAGAC
BbvCI 876 CCTCAGC CCTCAGC(-5/-2)
1278 CCTCAGC
BglI 1455 GCCCTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1771 GCCGCCGAGGC
BpuAI 450 GAAGAC GAAGAC(2/6)
957 GAAGAC
BsaAI 1072 CACGTG [CT]AC^GT[GA]
1972 TACGTG
BseRI 1299 CTCCTC GAGGAG(10/8)
1474 GAGGAG
BsiEI 309 CGGCCG CG[GA][CT]^CG
1408 CGGCCG
BsmBI 1848 CGTCTC CGTCTC(1/5)
271 GAGACG
BspLU11I 2408 ACATGT A^CATGT
2516 ACATGT
BstYI 2276 AGATCT [GA]^GATC[CT]
2763 GGATCC
EagI 309 CGGCCG C^GGCCG
1408 CGGCCG
EclXI 309 CGGCCG C^GGCCG
1408 CGGCCG
Eco52I 309 CGGCCG C^GGCCG
1408 CGGCCG
MflI 2276 AGATCT [GA]^GATC[CT]
2763 GGATCC
NcoI 828 CCATGG C^CATGG
2834 CCATGG
NotI 308 GCGGCCGC GC^GGCCGC
1407 GCGGCCGC
SmaI 899 CCCGGG CCC^GGG
2691 CCCGGG
XhoII 2276 AGATCT [GA]^GATC[CT]
2763 GGATCC
XmaCI 899 CCCGGG C^CCGGG
2691 CCCGGG
XmaI 899 CCCGGG C^CCGGG
2691 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI