Restriction map for
pF1KE0392
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AvrII BbvCI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsiEI BsiWI Bsp1407I BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I CelII CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI HpaI KpnI MfeI MluI
MunI NdeI NotI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
PvuI RcaI RsrII SalI SapI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI StuI SwaI
XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 760 AACGTT AA^CGTT
AcsI 882 GAATTC [GA]^AATT[CT]
AflIII 1147 ACATGT A^C[GA][CT]GT
Alw44I 1493 GTGCAC G^TGCAC
AlwNI 1585 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1889 GGGCCC GGGCC^C
ApaLI 1493 GTGCAC G^TGCAC
ApoI 882 GAATTC [GA]^AATT[CT]
Asp700I 85 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1669 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1877 TGCGCA TGC^GCA
BalI 1947 TGGCCA TGG^CCA
BamHI 1535 GGATCC G^GATCC
BbrPI 2656 CACGTG CAC^GTG
BciVI 699 GGATAC GTATCC(6/5)
BsaBI 1326 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1861 CTGCAC GTGCAG(16/14)
BsmI 1800 GAATGC GAATGC(1/-1)
BspDI 370 ATCGAT AT^CGAT
BspLU11I 1147 ACATGT A^CATGT
BsrBI 1737 CCGCTC CCGCTC(-3/-3)
BstAPI 2652 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 370 ATCGAT AT^CGAT
DraIII 2215 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 882 GAATTC G^AATTC
FspI 1877 TGCGCA TGC^GCA
HincII 1212 GTTGAC GT[CT]^[GA]AC
HindII 1212 GTTGAC GT[CT]^[GA]AC
HindIII 467 AAGCTT A^AGCTT
MamI 1326 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1947 TGGCCA TGG^CCA
MscI 1947 TGGCCA TGG^CCA
NaeI 2605 GCCGGC GCC^GGC
NgoMIV 2605 GCCGGC G^CCGGC
NheI 2133 GCTAGC G^CTAGC
NruI 603 TCGCGA TCG^CGA
NspI 1147 ACATGT [GA]CATG^[CT]
PflFI 1669 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2743 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2656 CACGTG CAC^GTG
PmlI 2656 CACGTG CAC^GTG
PpuMI 149 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1789 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 760 AACGTT AA^CGTT
SbfI 498 CCTGCAGG CCTGCA^GG
SexAI 1757 ACCTGGT A^CC[AT]GGT
Sse8387I 498 CCTGCAGG CCTGCA^GG
SspI 906 AATATT AAT^ATT
Tth111I 1669 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2743 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 963 CCATGATGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 85 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1296 GTCTAC GT^[AC][GT]AC
2680 GTCTAC
AccIII 477 TCCGGA T^CCGGA
842 TCCGGA
BbeI 6 GGCGCC GGCGC^C
162 GGCGCC
BbsI 1477 GAAGAC GAAGAC(2/6)
2107 GTCTTC
BglII 44 AGATCT A^GATCT
292 AGATCT
BpuAI 1477 GAAGAC GAAGAC(2/6)
2107 GTCTTC
BsaAI 742 CACGTA [CT]AC^GT[GA]
2656 CACGTG
BsaWI 477 TCCGGA [AT]^CCGG[AT]
842 TCCGGA
BseAI 477 TCCGGA T^CCGGA
842 TCCGGA
BspEI 477 TCCGGA T^CCGGA
842 TCCGGA
BsrFI 2500 ACCGGC [GA]^CCGG[CT]
2605 GCCGGC
BssSI 2263 CACGAG CACGAG(-5/-1)
2077 CTCGTG
Cfr10I 2500 ACCGGC [GA]^CCGG[CT]
2605 GCCGGC
KasI 6 GGCGCC G^GCGCC
162 GGCGCC
KspI 257 CCGCGG CCGC^GG
2279 CCGCGG
MroI 477 TCCGGA T^CCGGA
842 TCCGGA
MseI 377 TTAA T^TAA
566 TTAA
NarI 6 GGCGCC GG^CGCC
162 GGCGCC
NcoI 1944 CCATGG C^CATGG
2612 CCATGG
PvuII 1585 CAGCTG CAG^CTG
2717 CAGCTG
SacI 280 GAGCTC GAGCT^C
2583 GAGCTC
SacII 257 CCGCGG CCGC^GG
2279 CCGCGG
SfoI 6 GGCGCC GGC^GCC
162 GGCGCC
SmaI 167 CCCGGG CCC^GGG
1083 CCCGGG
Tru9I 377 TTAA T^TAA
566 TTAA
XmaCI 167 CCCGGG C^CCGGG
1083 CCCGGG
XmaI 167 CCCGGG C^CCGGG
1083 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII