Restriction map for
pF1KE0391
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AocI ApaLI
AscI Asp718I AspEI AviII AvrII
BbeI BcgI BciVI BglI BlnI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BsrFI BssHII BssSI BstBI
BstUI BstXI Bsu36I Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoT14I
FauI FseI FspI Hin1I HindIII
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PvuI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI StuI
StyI SwaI Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AclI 1394 AACGTT AA^CGTT
AlwNI 649 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 855 AGCGCT AGC^GCT
ApaI 678 GGGCCC GGGCC^C
AseI 772 ATTAAT AT^TAAT
AsnI 772 ATTAAT AT^TAAT
Asp700I 3329 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2154 GTGCTC G[AT]GC[AT]^C
AspI 2142 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 500 GGATCC G^GATCC
BbrPI 1143 CACGTG CAC^GTG
BbvCI 2098 GCTGAGG CCTCAGC(-5/-2)
BclI 3207 TGATCA T^GATCA
BglII 1748 AGATCT A^GATCT
BlpI 2438 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2438 GCTTAGC GC^T[ACGT]AGC
BsiHKAI 2154 GTGCTC G[AT]GC[AT]^C
Bsp1407I 3371 TGTACA T^GTACA
BspHI 2636 TCATGA T^CATGA
BspLU11I 338 ACATGT A^CATGT
BsrDI 142 GCAATG GCAATG(2/0)
BsrGI 3371 TGTACA T^GTACA
BstEII 3017 GGTTACC G^GT[ACGT]ACC
BstPI 3017 GGTTACC G^GT[ACGT]ACC
CelII 2438 GCTTAGC GC^T[ACGT]AGC
DraI 2622 TTTAAA TTT^AAA
DsaI 872 CCGTGG C^C[GA][CT]GG
Eco47III 855 AGCGCT AGC^GCT
EcoO65I 3017 GGTTACC G^GT[ACGT]ACC
EcoRV 1674 GATATC GAT^ATC
EcoT22I 3469 ATGCAT ATGCA^T
FbaI 3207 TGATCA T^GATCA
HgaI 2586 GACGC GACGC(5/10)
MslI 157 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 2835 GCTAGC G^CTAGC
NsiI 3469 ATGCAT ATGCA^T
PflFI 2142 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1143 CACGTG CAC^GTG
PmlI 1143 CACGTG CAC^GTG
PpuMI 3002 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 772 ATTAAT AT^TAAT
Psp1406I 1394 AACGTT AA^CGTT
PvuII 2197 CAGCTG CAG^CTG
RcaI 2636 TCATGA T^CATGA
SapI 3025 GCTCTTC GCTCTTC(1/4)
SbfI 1717 CCTGCAGG CCTGCA^GG
SpeI 561 ACTAGT A^CTAGT
SphI 2069 GCATGC GCATG^C
Sse8387I 1717 CCTGCAGG CCTGCA^GG
SspBI 3371 TGTACA T^GTACA
Tth111I 2142 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1605 TCTAGA T^CTAGA
XcmI 1680 CCAAATTTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3329 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 2305 GTAC GT^AC
3372 GTAC
AflII 766 CTTAAG C^TTAAG
3310 CTTAAG
AflIII 338 ACATGT A^C[GA][CT]GT
1142 ACACGT
AvaI 2399 CCCGAG C^[CT]CG[GA]G
2804 CTCGGG
AvaII 78 GGACC G^G[AT]CC
3003 GGACC
BanI 2222 GGCACC G^G[CT][GA]CC
2432 GGTGCC
BanII 678 GGGCCC G[GA]GC[CT]^C
2807 GGGCTC
BfrI 766 CTTAAG C^TTAAG
3310 CTTAAG
BsaAI 1143 CACGTG [CT]AC^GT[GA]
1505 TACGTG
BsmFI 2558 GGGAC GGGAC(10/14)
1933 GTCCC
BsoBI 2399 CCCGAG C^[CT]CG[GA]G
2804 CTCGGG
BspMI 1716 ACCTGC ACCTGC(4/8)
3021 ACCTGC
Bst1107I 606 GTATAC GTA^TAC
3537 GTATAC
BstAPI 2233 GCATACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2455 GCATTTGTTGC
BstZ17I 606 GTATAC GTA^TAC
3537 GTATAC
DraII 677 AGGGCCC [GA]G^G[ACGT]CC[CT]
3002 AGGACCC
EcoO109I 677 AGGGCCC [GA]G^G[ACGT]CC[CT]
3002 AGGACCC
EcoRI 394 GAATTC G^AATTC
2578 GAATTC
HaeII 644 AGCGCC [GA]GCGC^[CT]
855 AGCGCT
HincII 2799 GTTAAC GT[CT]^[GA]AC
3478 GTTAAC
HindII 2799 GTTAAC GT[CT]^[GA]AC
3478 GTTAAC
HpaI 2799 GTTAAC GTT^AAC
3478 GTTAAC
MspA1I 2159 CCGCTG C[AC]G^C[GT]G
2197 CAGCTG
RsaI 2305 GTAC GT^AC
3372 GTAC
TaqI 2080 TCGA T^CGA
3010 TCGA
TthHB8I 2080 TCGA T^CGA
3010 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AciI AcsI AluI AlwI
ApoI BalI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII