Restriction map for
pF1KE0389
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII Alw44I Aor51HI ApaLI
ApoI AseI AsnI Asp700I Asp718I
AviII AvrII BbeI BbrPI BcgI
BciVI BfrI BlnI BsaBI BsaHI
BseAI BsiWI Bsp1407I BspEI BspLU11I
BsrGI Bst1107I BstAPI BstBI BstXI
BstZ17I DraI DraIII Eco47III EcoRI
EcoRV EcoT22I FspI HaeII Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NarI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tsp509I XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1669 GTCTAC GT^[AC][GT]AC
AflIII 1213 ACACGT A^C[GA][CT]GT
AgeI 1259 ACCGGT A^CCGGT
AhdI 292 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 60 GGCGCGCC GG^CGCGCC
AspEI 292 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 971 GGATCC G^GATCC
BbsI 2088 GAAGAC GAAGAC(2/6)
BbvCI 1843 GCTGAGG CCTCAGC(-5/-2)
BclI 1817 TGATCA T^GATCA
BglI 1496 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1850 AGATCT A^GATCT
BpuAI 2088 GAAGAC GAAGAC(2/6)
BsaAI 1033 TACGTG [CT]AC^GT[GA]
BsaWI 1259 ACCGGT [AT]^CCGG[AT]
BsmBI 789 CGTCTC CGTCTC(1/5)
BsmI 1810 GCATTC GAATGC(1/-1)
BspDI 499 ATCGAT AT^CGAT
ClaI 499 ATCGAT AT^CGAT
CpoI 447 CGGTCCG CG^G[AT]CCG
DrdI 1993 GACTTGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 292 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1960 CTCTTC CTCTTC(1/4)
EcoNI 1860 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1817 TGATCA T^GATCA
FseI 3 GGCCGGCC GGCCGG^CC
HgaI 788 GCGTC GACGC(5/10)
Ksp632I 1960 CTCTTC CTCTTC(1/4)
MseI 1772 TTAA T^TAA
NaeI 4 GCCGGC GCC^GGC
NdeI 2334 CATATG CA^TATG
NgoMIV 4 GCCGGC G^CCGGC
PflMI 990 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1259 ACCGGT A^CCGGT
RsrII 447 CGGTCCG CG^G[AT]CCG
SacI 742 GAGCTC GAGCT^C
SapI 1959 GCTCTTC GCTCTTC(1/4)
SexAI 1748 ACCAGGT A^CC[AT]GGT
SmlI 1044 CTTGAG C^T[CT][GA]AG
SspI 286 AATATT AAT^ATT
StuI 1580 AGGCCT AGG^CCT
Tru9I 1772 TTAA T^TAA
Van91I 990 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1131 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 29 enzymes) #
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ApaI 2100 GGGCCC GGGCC^C
2345 GGGCCC
AspHI 742 GAGCTC G[AT]GC[AT]^C
1873 GTGCTC
AspI 469 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1599 GACGGTGTC
BalI 1451 TGGCCA TGG^CCA
2363 TGGCCA
BlpI 692 GCTCAGC GC^T[ACGT]AGC
1368 GCTCAGC
Bpu1102I 692 GCTCAGC GC^T[ACGT]AGC
1368 GCTCAGC
BsaI 1745 GAGACC GGTCTC(1/5)
21 GGTCTC
BsgI 770 CTGCAC GTGCAG(16/14)
1248 CTGCAC
BsiEI 492 CGGCCG CG[GA][CT]^CG
2287 CGGCCG
BsiHKAI 742 GAGCTC G[AT]GC[AT]^C
1873 GTGCTC
BspHI 746 TCATGA T^CATGA
1814 TCATGA
BsrDI 630 CATTGC GCAATG(2/0)
536 GCAATG
BssHII 61 GCGCGC G^CGCGC
1806 GCGCGC
BstEII 1542 GGTGACC G^GT[ACGT]ACC
1644 GGTGACC
BstPI 1542 GGTGACC G^GT[ACGT]ACC
1644 GGTGACC
CelII 692 GCTCAGC GC^T[ACGT]AGC
1368 GCTCAGC
EagI 492 CGGCCG C^GGCCG
2287 CGGCCG
EclXI 492 CGGCCG C^GGCCG
2287 CGGCCG
Eco52I 492 CGGCCG C^GGCCG
2287 CGGCCG
EcoO65I 1542 GGTGACC G^GT[ACGT]ACC
1644 GGTGACC
HincII 1039 GTTGAC GT[CT]^[GA]AC
1912 GTCAAC
HindII 1039 GTTGAC GT[CT]^[GA]AC
1912 GTCAAC
HindIII 83 AAGCTT A^AGCTT
1887 AAGCTT
MluNI 1451 TGGCCA TGG^CCA
2363 TGGCCA
MscI 1451 TGGCCA TGG^CCA
2363 TGGCCA
PflFI 469 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1599 GACGGTGTC
RcaI 746 TCATGA T^CATGA
1814 TCATGA
Tth111I 469 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1599 GACGGTGTC
XcmI 1742 CCAGAGACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2427 CCATCCCAACAATGG
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII
XmaCI XmaI