Restriction map for
pF1KE0387
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BbrPI
BbvCI BcgI BciVI BclI BlnI
BsaAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspLU11I BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I EarI Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI Ksp632I KspI MfeI
MluI MluNI MscI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacI SacII
SapI ScaI SexAI SfiI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 239 TCCGGA T^CCGGA
AclI 183 AACGTT AA^CGTT
AcyI 141 GGCGCC G[GA]^CG[CT]C
AflII 1168 CTTAAG C^TTAAG
Aor51HI 413 AGCGCT AGC^GCT
Asp700I 978 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 887 GGATCC G^GATCC
BbeI 141 GGCGCC GGCGC^C
BfaI 1453 CTAG C^TAG
BfrI 1168 CTTAAG C^TTAAG
BglI 673 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1019 AGATCT A^GATCT
BlpI 250 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 250 GCTCAGC GC^T[ACGT]AGC
BsaBI 888 GATCCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 141 GGCGCC G[GA]^CG[CT]C
BsaI 1541 GAGACC GGTCTC(1/5)
BsaWI 239 TCCGGA [AT]^CCGG[AT]
BseAI 239 TCCGGA T^CCGGA
BseRI 481 CTCCTC GAGGAG(10/8)
BsgI 1412 GTGCAG GTGCAG(16/14)
BsiEI 1142 CGGCCG CG[GA][CT]^CG
BsmI 662 GAATGC GAATGC(1/-1)
BspEI 239 TCCGGA T^CCGGA
BspHI 1721 TCATGA T^CATGA
BsrBI 97 GAGCGG CCGCTC(-3/-3)
BsrDI 759 CATTGC GCAATG(2/0)
BstBI 626 TTCGAA TT^CGAA
CelII 250 GCTCAGC GC^T[ACGT]AGC
EagI 1142 CGGCCG C^GGCCG
EclXI 1142 CGGCCG C^GGCCG
Eco47III 413 AGCGCT AGC^GCT
Eco52I 1142 CGGCCG C^GGCCG
EcoNI 1091 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 385 GATATC GAT^ATC
EcoT14I 1433 CCTTGG C^C[AT][AT]GG
Hin1I 141 GGCGCC G[GA]^CG[CT]C
KasI 141 GGCGCC G^GCGCC
MaeI 1453 CTAG C^TAG
MamI 888 GATCCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 239 TCCGGA T^CCGGA
NarI 141 GGCGCC GG^CGCC
NspI 1496 ACATGC [GA]CATG^[CT]
NspV 626 TTCGAA TT^CGAA
Psp1406I 183 AACGTT AA^CGTT
RcaI 1721 TCATGA T^CATGA
SbfI 24 CCTGCAGG CCTGCA^GG
SfoI 141 GGCGCC GGC^GCC
SfuI 626 TTCGAA TT^CGAA
SgrAI 143 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1168 CTTAAG C^T[CT][GA]AG
Sse8387I 24 CCTGCAGG CCTGCA^GG
StyI 1433 CCTTGG C^C[AT][AT]GG
XmnI 978 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 170 GTCGAC GT^[AC][GT]AC
547 GTCGAC
AccII 94 CGCG CG^CG
256 CGCG
AcsI 1342 AAATTC [GA]^AATT[CT]
1795 AAATTC
AlwNI 205 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1756 CAGTGGCTG
ApoI 1342 AAATTC [GA]^AATT[CT]
1795 AAATTC
AspHI 502 GTGCTC G[AT]GC[AT]^C
568 GAGCAC
BbsI 278 GAAGAC GAAGAC(2/6)
589 GAAGAC
BpuAI 278 GAAGAC GAAGAC(2/6)
589 GAAGAC
BsiHKAI 502 GTGCTC G[AT]GC[AT]^C
568 GAGCAC
BstAPI 838 GCAGGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1688 GCATGGACTGC
BstUI 94 CGCG CG^CG
256 CGCG
DraIII 496 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
713 CACACTGTG
HgaI 247 GACGC GACGC(5/10)
1463 GCGTC
MaeIII 1597 GTCAC ^GT[ACGT]AC
1706 GTGAC
MvnI 94 CGCG CG^CG
256 CGCG
NaeI 144 GCCGGC GCC^GGC
1223 GCCGGC
NgoMIV 144 GCCGGC G^CCGGC
1223 GCCGGC
PleI 937 GAGTC GAGTC(4/5)
1148 GAGTC
PvuII 1633 CAGCTG CAG^CTG
1683 CAGCTG
SalI 170 GTCGAC G^TCGAC
547 GTCGAC
Tsp45I 1597 GTCAC ^GT[GC]AC
1706 GTGAC
XcmI 1239 CCACCTCTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1668 CCACCCTGTCAGTGG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII