Restriction map for
pF1KE0386
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII AccII AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbeI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsiEI
BsmBI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 2076 GGTACC G^GTACC
AcsI 802 GAATTC [GA]^AATT[CT]
AcyI 208 GACGCC G[GA]^CG[CT]C
Alw44I 1971 GTGCAC G^TGCAC
AocI 1138 CCTCAGG CC^T[ACGT]AGG
ApaLI 1971 GTGCAC G^TGCAC
ApoI 802 GAATTC [GA]^AATT[CT]
Asp718I 2076 GGTACC G^GTACC
AspI 1113 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 745 CTCGGG C^[CT]CG[GA]G
BamHI 1883 GGATCC G^GATCC
BbrPI 1438 CACGTG CAC^GTG
BglI 1893 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 208 GACGCC G[GA]^CG[CT]C
BsgI 957 GTGCAG GTGCAG(16/14)
BsiWI 242 CGTACG C^GTACG
BsoBI 745 CTCGGG C^[CT]CG[GA]G
Bsp1407I 878 TGTACA T^GTACA
BspDI 172 ATCGAT AT^CGAT
BsrDI 395 GCAATG GCAATG(2/0)
BsrFI 1401 GCCGGT [GA]^CCGG[CT]
BsrGI 878 TGTACA T^GTACA
Bsu36I 1138 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1401 GCCGGT [GA]^CCGG[CT]
ClaI 172 ATCGAT AT^CGAT
DrdI 58 GACAGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1138 CCTCAGG CC^T[ACGT]AGG
EcoRI 802 GAATTC G^AATTC
HaeII 1398 AGCGCC [GA]GCGC^[CT]
Hin1I 208 GACGCC G[GA]^CG[CT]C
KpnI 2076 GGTACC GGTAC^C
MseI 607 TTAA T^TAA
NcoI 150 CCATGG C^CATGG
PflFI 1113 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1438 CACGTG CAC^GTG
PmlI 1438 CACGTG CAC^GTG
PshAI 2004 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1408 GAGCTC GAGCT^C
ScaI 1172 AGTACT AGT^ACT
SgrAI 1400 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 878 TGTACA T^GTACA
StuI 810 AGGCCT AGG^CCT
Tru9I 607 TTAA T^TAA
Tth111I 1113 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 375 TCTAGA T^CTAGA
XcmI 269 CCAGCCCTTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 382 GTAGAC GT^[AC][GT]AC
664 GTCTAC
AspHI 1408 GAGCTC G[AT]GC[AT]^C
1971 GTGCAC
BbsI 1719 GAAGAC GAAGAC(2/6)
916 GTCTTC
BfaI 376 CTAG C^TAG
622 CTAG
BpuAI 1719 GAAGAC GAAGAC(2/6)
916 GTCTTC
BsaI 294 GGTCTC GGTCTC(1/5)
369 GGTCTC
BseRI 742 CTCCTC GAGGAG(10/8)
841 GAGGAG
BsiHKAI 1408 GAGCTC G[AT]GC[AT]^C
1971 GTGCAC
BsmI 787 GCATTC GAATGC(1/-1)
1694 GCATTC
BstXI 150 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1049 CCAACCGCCTGG
CfoI 1337 GCGC GCG^C
1399 GCGC
FauI 1296 CCCGC CCCGC(4/6)
113 GCGGG
HgaI 208 GACGC GACGC(5/10)
1570 GACGC
HhaI 1337 GCGC GCG^C
1399 GCGC
HinP1I 1337 GCGC G^CGC
1399 GCGC
MaeI 376 CTAG C^TAG
622 CTAG
PstI 967 CTGCAG CTGCA^G
973 CTGCAG
SapI 1011 GAAGAGC GCTCTTC(1/4)
1573 GCTCTTC
SmlI 514 CTTGAG C^T[CT][GA]AG
848 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaII BalI BanI BanII
BbvI BcnI BmyI BpmI BsaAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII