Restriction map for
pF1KE0384
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbrPI BcgI BfrI BglII
BlnI BsaAI BsaI BseAI BseRI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspMI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
MfeI MluI MluNI MroI MscI
MslI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 781 GACGTC GACGT^C
AflIII 878 ACATGT A^C[GA][CT]GT
Alw44I 252 GTGCAC G^TGCAC
ApaLI 252 GTGCAC G^TGCAC
BamHI 1220 GGATCC G^GATCC
BbsI 1393 GAAGAC GAAGAC(2/6)
BciVI 1245 GGATAC GTATCC(6/5)
BclI 1052 TGATCA T^GATCA
BfaI 1021 CTAG C^TAG
BlpI 802 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 802 GCTGAGC GC^T[ACGT]AGC
BpuAI 1393 GAAGAC GAAGAC(2/6)
BsaWI 1177 TCCGGT [AT]^CCGG[AT]
BsmAI 637 GTCTC GTCTC(1/5)
BsmI 919 GCATTC GAATGC(1/-1)
BspLU11I 878 ACATGT A^CATGT
BsrBI 453 GAGCGG CCGCTC(-3/-3)
BstAPI 213 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1220 GGATCC [GA]^GATC[CT]
CelII 802 GCTGAGC GC^T[ACGT]AGC
EagI 353 CGGCCG C^GGCCG
EarI 328 GAAGAG CTCTTC(1/4)
EclXI 353 CGGCCG C^GGCCG
Eco52I 353 CGGCCG C^GGCCG
EcoNI 585 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1052 TGATCA T^GATCA
HinfI 520 GACTC G^A[ACGT]TC
Ksp632I 328 GAAGAG CTCTTC(1/4)
KspI 200 CCGCGG CCGC^GG
MaeI 1021 CTAG C^TAG
MflI 1220 GGATCC [GA]^GATC[CT]
MseI 1304 TTAA T^TAA
NaeI 749 GCCGGC GCC^GGC
NgoMIV 749 GCCGGC G^CCGGC
PleI 520 GACTC GAGTC(4/5)
SacII 200 CCGCGG CCGC^GG
SapI 328 GAAGAGC GCTCTTC(1/4)
SbfI 786 CCTGCAGG CCTGCA^GG
Sse8387I 786 CCTGCAGG CCTGCA^GG
Tru9I 1304 TTAA T^TAA
Tsp45I 1289 GTGAC ^GT[GC]AC
XcmI 38 CCAGCGTGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1220 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 882 GTAC GT^AC
1015 GTAC
AlwI 1221 GATCC GGATC(4/5)
1220 GGATC
BbvCI 732 CCTCAGC CCTCAGC(-5/-2)
821 CCTCAGC
BsaBI 852 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1221 GATCCTTATC
BsgI 151 CTGCAC GTGCAG(16/14)
1356 CTGCAC
BsrDI 1293 CATTGC GCAATG(2/0)
1347 CATTGC
MaeII 782 ACGT A^CGT
1379 ACGT
MaeIII 869 GTAAC ^GT[ACGT]AC
1289 GTGAC
MamI 852 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1221 GATCCTTATC
MboII 328 GAAGA GAAGA(8/7)
1393 GAAGA
NcoI 500 CCATGG C^CATGG
1040 CCATGG
NspI 878 ACATGT [GA]CATG^[CT]
932 GCATGT
PflMI 699 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1335 CCAGGTCCTGG
PpuMI 817 GGGTCCT [GA]G^G[AT]CC[CT]
1337 AGGTCCT
RsaI 882 GTAC GT^AC
1015 GTAC
SacI 424 GAGCTC GAGCT^C
910 GAGCTC
StuI 1277 AGGCCT AGG^CCT
1409 AGGCCT
TaqI 578 TCGA T^CGA
1439 TCGA
TthHB8I 578 TCGA T^CGA
1439 TCGA
Van91I 699 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1335 CCAGGTCCTGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
StyI TseI TspRI XmaCI XmaI