Restriction map for
pF1KE0380
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BbeI BbsI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraIII DrdI EcoNI EcoO65I EcoRI
FbaI FseI FspI HindIII HpaI
KasI KpnI MfeI MluI MroI
MunI NarI NcoI NdeI NheI
NruI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfoI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 645 GGCGTC G[GA]^CG[CT]C
AflIII 1469 ACACGT A^C[GA][CT]GT
AhdI 1412 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 866 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 1412 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 151 GGACC G^G[AT]CC
BamHI 731 GGATCC G^GATCC
BanI 207 GGTGCC G^G[CT][GA]CC
BbrPI 855 CACGTG CAC^GTG
BbvCI 318 CCTCAGC CCTCAGC(-5/-2)
BcgI 1563 CGAAGAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 502 CTAG C^TAG
BsaAI 855 CACGTG [CT]AC^GT[GA]
BsaBI 306 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 645 GGCGTC G[GA]^CG[CT]C
BsaI 917 GGTCTC GGTCTC(1/5)
BsrDI 1518 CATTGC GCAATG(2/0)
BssHII 227 GCGCGC G^CGCGC
BstBI 793 TTCGAA TT^CGAA
DraI 1171 TTTAAA TTT^AAA
Eam1105I 1412 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 739 GAAGAG CTCTTC(1/4)
EcoRV 493 GATATC GAT^ATC
EcoT14I 584 CCAAGG C^C[AT][AT]GG
EcoT22I 1552 ATGCAT ATGCA^T
HgaI 646 GCGTC GACGC(5/10)
Hin1I 645 GGCGTC G[GA]^CG[CT]C
HincII 447 GTCAAC GT[CT]^[GA]AC
HindII 447 GTCAAC GT[CT]^[GA]AC
HphI 765 GGTGA GGTGA(8/7)
Ksp632I 739 GAAGAG CTCTTC(1/4)
MaeI 502 CTAG C^TAG
MamI 306 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 210 GCCGGC GCC^GGC
NgoMIV 210 GCCGGC G^CCGGC
NotI 198 GCGGCCGC GC^GGCCGC
NsiI 1552 ATGCAT ATGCA^T
NspV 793 TTCGAA TT^CGAA
PflMI 1401 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 855 CACGTG CAC^GTG
PmlI 855 CACGTG CAC^GTG
PpuMI 150 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1177 GAGCTC GAGCT^C
SexAI 1415 ACCAGGT A^CC[AT]GGT
SfuI 793 TTCGAA TT^CGAA
SgrAI 203 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 172 CCCGGG CCC^GGG
SmlI 478 CTTGAG C^T[CT][GA]AG
SspI 959 AATATT AAT^ATT
StuI 1324 AGGCCT AGG^CCT
StyI 584 CCAAGG C^C[AT][AT]GG
Van91I 1401 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 172 CCCGGG C^CCGGG
XmaI 172 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 1021 GAATTT [GA]^AATT[CT]
1168 GAATTT
AfaI 1428 GTAC GT^AC
1467 GTAC
AocI 1315 CCTGAGG CC^T[ACGT]AGG
1507 CCTGAGG
Aor51HI 356 AGCGCT AGC^GCT
554 AGCGCT
ApoI 1021 GAATTT [GA]^AATT[CT]
1168 GAATTT
Asp700I 786 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1716 GAAATGGTTC
AvaI 172 CCCGGG C^[CT]CG[GA]G
352 CCCGAG
BsoBI 172 CCCGGG C^[CT]CG[GA]G
352 CCCGAG
Bsu36I 1315 CCTGAGG CC^T[ACGT]AGG
1507 CCTGAGG
DraII 150 GGGACCC [GA]G^G[ACGT]CC[CT]
617 AGGGCCT
DsaI 31 CCGCGG C^C[GA][CT]GG
196 CCGCGG
EagI 48 CGGCCG C^GGCCG
199 CGGCCG
EclXI 48 CGGCCG C^GGCCG
199 CGGCCG
Eco47III 356 AGCGCT AGC^GCT
554 AGCGCT
Eco52I 48 CGGCCG C^GGCCG
199 CGGCCG
Eco81I 1315 CCTGAGG CC^T[ACGT]AGG
1507 CCTGAGG
EcoO109I 150 GGGACCC [GA]G^G[ACGT]CC[CT]
617 AGGGCCT
KspI 31 CCGCGG CCGC^GG
196 CCGCGG
MaeII 856 ACGT A^CGT
1471 ACGT
MseI 308 TTAA T^TAA
1172 TTAA
MslI 1064 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1518 CATTGCTGTG
NspI 1308 GCATGT [GA]CATG^[CT]
1736 GCATGT
PleI 745 GAGTC GAGTC(4/5)
1660 GAGTC
PvuII 253 CAGCTG CAG^CTG
1148 CAGCTG
RsaI 1428 GTAC GT^AC
1467 GTAC
SacII 31 CCGCGG CCGC^GG
196 CCGCGG
TfiI 1452 GAATC G^A[AT]TC
1581 GAATC
Tru9I 308 TTAA T^TAA
1172 TTAA
XmnI 786 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1716 GAAATGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
BalI BanII BbvI BciVI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII