Restriction map for
pF1KB6113
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII ClaI CpoI
DraIII DsaI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI Hin1I KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1759 GTCTAC GT^[AC][GT]AC
AcsI 1555 AAATTC [GA]^AATT[CT]
AflIII 1755 ACACGT A^C[GA][CT]GT
AocI 559 CCTCAGG CC^T[ACGT]AGG
Aor51HI 299 AGCGCT AGC^GCT
ApoI 1555 AAATTC [GA]^AATT[CT]
AvaII 158 GGTCC G^G[AT]CC
BciVI 1005 GTATCC GTATCC(6/5)
BclI 1527 TGATCA T^GATCA
BglI 106 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 1255 GTGCAG GTGCAG(16/14)
BsmFI 1353 GGGAC GGGAC(10/14)
BsmI 450 GAATGC GAATGC(1/-1)
Bsp1407I 1730 TGTACA T^GTACA
BsrDI 528 CATTGC GCAATG(2/0)
BsrFI 293 GCCGGC [GA]^CCGG[CT]
BsrGI 1730 TGTACA T^GTACA
BstAPI 212 GCATCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 559 CCTCAGG CC^T[ACGT]AGG
Cfr10I 293 GCCGGC [GA]^CCGG[CT]
DraI 76 TTTAAA TTT^AAA
DraII 1283 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 1820 GACTGCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1804 CGGCCA [CT]^GGCC[GA]
Eco47III 299 AGCGCT AGC^GCT
Eco81I 559 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1283 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 836 CCAAGG C^C[AT][AT]GG
FauI 1350 GCGGG CCCGC(4/6)
FbaI 1527 TGATCA T^GATCA
HaeII 299 AGCGCT [GA]GCGC^[CT]
HgaI 1827 GCGTC GACGC(5/10)
HindIII 412 AAGCTT A^AGCTT
HpaI 96 GTTAAC GTT^AAC
MaeII 1757 ACGT A^CGT
NaeI 293 GCCGGC GCC^GGC
NgoMIV 293 GCCGGC G^CCGGC
ScaI 590 AGTACT AGT^ACT
SspBI 1730 TGTACA T^GTACA
StuI 984 AGGCCT AGG^CCT
StyI 836 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 439 GATCC GGATC(4/5)
556 GATCC
Asp700I 56 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
748 GAAAAACTTC
BanI 348 GGTGCC G^G[CT][GA]CC
1549 GGCACC
BanII 166 GAGCTC G[GA]GC[CT]^C
1084 GAGCTC
BbsI 484 GAAGAC GAAGAC(2/6)
1705 GAAGAC
BcnI 1560 CCCGG CC^[GC]GG
1739 CCCGG
BpuAI 484 GAAGAC GAAGAC(2/6)
1705 GAAGAC
BsaI 1369 GAGACC GGTCTC(1/5)
1791 GAGACC
BsaJI 836 CCAAGG C^C[ACGT][ACGT]GG
1738 CCCCGG
BsaWI 1001 TCCGGT [AT]^CCGG[AT]
1717 ACCGGA
BseRI 980 GAGGAG GAGGAG(10/8)
1213 GAGGAG
BstXI 969 CCAACATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1289 CCATCTGCCTGG
EcoNI 1275 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1416 CCTGGAAGAGG
HincII 96 GTTAAC GT[CT]^[GA]AC
1829 GTCAAC
HindII 96 GTTAAC GT[CT]^[GA]AC
1829 GTCAAC
MslI 1089 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1485 CATCAAGGTG
NciI 1560 CCCGG CC^[GC]GG
1739 CCCGG
NspI 1382 GCATGT [GA]CATG^[CT]
1426 GCATGT
PstI 806 CTGCAG CTGCA^G
1364 CTGCAG
PvuII 403 CAGCTG CAG^CTG
1258 CAGCTG
SacI 166 GAGCTC GAGCT^C
1084 GAGCTC
Tsp45I 203 GTGAC ^GT[GC]AC
991 GTGAC
XmnI 56 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
748 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AspHI BbvI
BmyI BpmI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XcmI