Restriction map for
pF1KE0356
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
BanI BbeI BbrPI BbvCI BcgI
BciVI BlpI Bpu1102I BsaAI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DraII DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RsrII SacII SalI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AclI 927 AACGTT AA^CGTT
AflII 802 CTTAAG C^TTAAG
AflIII 543 ACATGT A^C[GA][CT]GT
AgeI 1131 ACCGGT A^CCGGT
AocI 1261 CCTCAGG CC^T[ACGT]AGG
AspHI 532 GAGCTC G[AT]GC[AT]^C
AvrII 1041 CCTAGG C^CTAGG
BalI 165 TGGCCA TGG^CCA
BamHI 974 GGATCC G^GATCC
BclI 116 TGATCA T^GATCA
BcnI 1148 CCCGG CC^[GC]GG
BfaI 1042 CTAG C^TAG
BfrI 802 CTTAAG C^TTAAG
BglII 906 AGATCT A^GATCT
BlnI 1041 CCTAGG C^CTAGG
BsaBI 1098 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 532 GAGCTC G[AT]GC[AT]^C
BspHI 572 TCATGA T^CATGA
BspLU11I 543 ACATGT A^CATGT
BspMI 1194 ACCTGC ACCTGC(4/8)
BsrDI 510 CATTGC GCAATG(2/0)
BsrFI 1131 ACCGGT [GA]^CCGG[CT]
BstAPI 142 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1261 CCTCAGG CC^T[ACGT]AGG
Cac8I 1422 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 1131 ACCGGT [GA]^CCGG[CT]
DraIII 1095 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1258 GACCCTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 165 TGGCCA [CT]^GGCC[GA]
EarI 1229 CTCTTC CTCTTC(1/4)
Eco81I 1261 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1041 CCTAGG C^C[AT][AT]GG
FbaI 116 TGATCA T^GATCA
HaeII 839 GGCGCT [GA]GCGC^[CT]
HgaI 1184 GACGC GACGC(5/10)
Ksp632I 1229 CTCTTC CTCTTC(1/4)
MaeI 1042 CTAG C^TAG
MamI 1098 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 165 TGGCCA TGG^CCA
MscI 165 TGGCCA TGG^CCA
MspA1I 1337 CAGCTG C[AC]G^C[GT]G
NciI 1148 CCCGG CC^[GC]GG
PinAI 1131 ACCGGT A^CCGGT
Psp1406I 927 AACGTT AA^CGTT
PstI 1196 CTGCAG CTGCA^G
PvuII 1337 CAGCTG CAG^CTG
RcaI 572 TCATGA T^CATGA
SacI 532 GAGCTC GAGCT^C
SapI 1228 GCTCTTC GCTCTTC(1/4)
SbfI 1195 CCTGCAGG CCTGCA^GG
SexAI 701 ACCTGGT A^CC[AT]GGT
SfcI 1196 CTGCAG C^T[GA][CT]AG
Sse8387I 1195 CCTGCAGG CCTGCA^GG
StuI 1441 AGGCCT AGG^CCT
StyI 1041 CCTAGG C^C[AT][AT]GG
Tsp45I 376 GTGAC ^GT[GC]AC
XcmI 153 CCACTGGTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 447 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
924 GAAAACGTTC
AvaII 253 GGACC G^G[AT]CC
786 GGACC
BbsI 1255 GAAGAC GAAGAC(2/6)
1053 GTCTTC
BglI 6 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
590 GCCACCAGGGC
BpuAI 1255 GAAGAC GAAGAC(2/6)
1053 GTCTTC
BsaWI 788 ACCGGA [AT]^CCGG[AT]
1131 ACCGGT
BsgI 127 CTGCAC GTGCAG(16/14)
140 CTGCAC
BsiYI 183 CCCAGTAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1244 CCTATTTCTGG
BslI 183 CCCAGTAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1244 CCTATTTCTGG
BsmI 1111 GAATGC GAATGC(1/-1)
440 GCATTC
CfoI 672 GCGC GCG^C
840 GCGC
Cfr13I 253 GGACC G^G[ACGT]CC
786 GGACC
HhaI 672 GCGC GCG^C
840 GCGC
HinP1I 672 GCGC G^CGC
840 GCGC
MaeII 525 ACGT A^CGT
928 ACGT
MaeIII 376 GTGAC ^GT[ACGT]AC
1152 GTTAC
NspI 62 GCATGT [GA]CATG^[CT]
543 ACATGT
PleI 625 GACTC GAGTC(4/5)
796 GAGTC
Sau96I 253 GGACC G^G[ACGT]CC
786 GGACC
SspI 450 AATATT AAT^ATT
1219 AATATT
TaqI 366 TCGA T^CGA
1157 TCGA
TthHB8I 366 TCGA T^CGA
1157 TCGA
XmnI 447 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
924 GAAAACGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BmyI BpmI
BsaJI BseRI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI ScrFI
SfaNI SmlI TfiI Tru9I TseI
Tsp509I TspRI XhoII