Restriction map for
pF1KE0332
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII BanI
BanII BbeI BbvCI BcgI BclI
BfrI BglI BglII BlpI BmyI
Bpu1102I BsaBI BsaI BseAI BseRI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII HindIII HpaI KasI
KpnI KspI MamI MluI MroI
MslI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PmeI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 341 CGCG CG^CG
AcsI 1467 AAATTT [GA]^AATT[CT]
AcyI 13 GACGCC G[GA]^CG[CT]C
AgeI 209 ACCGGT A^CCGGT
AocI 300 CCTGAGG CC^T[ACGT]AGG
ApoI 1467 AAATTT [GA]^AATT[CT]
AseI 828 ATTAAT AT^TAAT
AsnI 828 ATTAAT AT^TAAT
AvrII 375 CCTAGG C^CTAGG
BamHI 71 GGATCC G^GATCC
BciVI 1267 GTATCC GTATCC(6/5)
BlnI 375 CCTAGG C^CTAGG
BsaHI 13 GACGCC G[GA]^CG[CT]C
BsaWI 209 ACCGGT [AT]^CCGG[AT]
BsrDI 1158 CATTGC GCAATG(2/0)
BsrFI 209 ACCGGT [GA]^CCGG[CT]
BssSI 535 CTCGTG CACGAG(-5/-1)
BstAPI 39 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1421 TTCGAA TT^CGAA
BstUI 341 CGCG CG^CG
Bsu36I 300 CCTGAGG CC^T[ACGT]AGG
CfoI 596 GCGC GCG^C
Cfr10I 209 ACCGGT [GA]^CCGG[CT]
DraII 370 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 117 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1500 CCACGG C^C[GA][CT]GG
EagI 1671 CGGCCG C^GGCCG
EarI 154 CTCTTC CTCTTC(1/4)
EclXI 1671 CGGCCG C^GGCCG
Eco52I 1671 CGGCCG C^GGCCG
Eco81I 300 CCTGAGG CC^T[ACGT]AGG
EcoO109I 370 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1753 GATATC GAT^ATC
HgaI 13 GACGC GACGC(5/10)
HhaI 596 GCGC GCG^C
Hin1I 13 GACGCC G[GA]^CG[CT]C
HinP1I 596 GCGC G^CGC
HincII 355 GTTGAC GT[CT]^[GA]AC
HindII 355 GTTGAC GT[CT]^[GA]AC
Ksp632I 154 CTCTTC CTCTTC(1/4)
MvnI 341 CGCG CG^CG
NdeI 971 CATATG CA^TATG
NspV 1421 TTCGAA TT^CGAA
PflMI 657 CCATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 209 ACCGGT A^CCGGT
PpuMI 370 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 828 ATTAAT AT^TAAT
PvuII 1733 CAGCTG CAG^CTG
SfuI 1421 TTCGAA TT^CGAA
SpeI 1804 ACTAGT A^CTAGT
SphI 1377 GCATGC GCATG^C
SspI 760 AATATT AAT^ATT
StuI 668 AGGCCT AGG^CCT
Van91I 657 CCATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1619 CCATTAAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 91 ACGTGT A^C[GA][CT]GT
1144 ACACGT
AlwNI 40 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1436 CAGAAACTG
AvaII 371 GGTCC G^G[AT]CC
1494 GGTCC
BbrPI 90 CACGTG CAC^GTG
1145 CACGTG
BcnI 198 CCCGG CC^[GC]GG
444 CCGGG
BsaAI 90 CACGTG [CT]AC^GT[GA]
1145 CACGTG
BsiEI 207 CGACCG CG[GA][CT]^CG
1671 CGGCCG
BsmI 1198 GCATTC GAATGC(1/-1)
1328 GCATTC
BspMI 930 ACCTGC ACCTGC(4/8)
39 GCAGGT
BstYI 71 GGATCC [GA]^GATC[CT]
1308 GGATCT
EcoNI 375 CCTAGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
426 CCTTGACAAGG
FauI 339 CCCGC CCCGC(4/6)
48 GCGGG
MfeI 818 CAATTG C^AATTG
1335 CAATTG
MflI 71 GGATCC [GA]^GATC[CT]
1308 GGATCT
MspA1I 134 CAGCGG C[AC]G^C[GT]G
1733 CAGCTG
MunI 818 CAATTG C^AATTG
1335 CAATTG
NciI 198 CCCGG CC^[GC]GG
444 CCGGG
PmaCI 90 CACGTG CAC^GTG
1145 CACGTG
PmlI 90 CACGTG CAC^GTG
1145 CACGTG
PstI 126 CTGCAG CTGCA^G
1442 CTGCAG
SmlI 586 CTTGAG C^T[CT][GA]AG
1229 CTCAAG
XhoII 71 GGATCC [GA]^GATC[CT]
1308 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI BalI
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I