Restriction map for
pF1KE0313
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
AccIII AclI AflIII AgeI Aor51HI
AscI AseI AsnI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BlnI BsaAI BsaBI BseAI BsiWI
BsmBI BspEI BspHI BspLU11I BssHII
BssSI BstBI CpoI DraI DrdI
DsaI EagI EclXI Eco47III Eco52I
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NotI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1438 GACGTC GACGT^C
Acc65I 1761 GGTACC G^GTACC
AccI 2829 GTATAC GT^[AC][GT]AC
AflII 2695 CTTAAG C^TTAAG
AhdI 1075 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1249 GTGCAC G^TGCAC
ApaLI 1249 GTGCAC G^TGCAC
Asp700I 2793 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1761 GGTACC G^GTACC
AspEI 1075 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1267 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1941 TGGCCA TGG^CCA
BbsI 544 GAAGAC GAAGAC(2/6)
BciVI 2095 GGATAC GTATCC(6/5)
BclI 1908 TGATCA T^GATCA
BfrI 2695 CTTAAG C^TTAAG
BglI 2147 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 326 AGATCT A^GATCT
BpuAI 544 GAAGAC GAAGAC(2/6)
BsmI 580 GAATGC GAATGC(1/-1)
Bsp1407I 2135 TGTACA T^GTACA
BspDI 1930 ATCGAT AT^CGAT
BsrDI 699 CATTGC GCAATG(2/0)
BsrGI 2135 TGTACA T^GTACA
Bst1107I 2829 GTATAC GTA^TAC
BstAPI 2019 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 798 GGTGACC G^GT[ACGT]ACC
BstPI 798 GGTGACC G^GT[ACGT]ACC
BstZ17I 2829 GTATAC GTA^TAC
ClaI 1930 ATCGAT AT^CGAT
DraIII 1252 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1941 TGGCCA [CT]^GGCC[GA]
Eam1105I 1075 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 798 GGTGACC G^GT[ACGT]ACC
EcoRI 2599 GAATTC G^AATTC
EcoRV 1060 GATATC GAT^ATC
FbaI 1908 TGATCA T^GATCA
KpnI 1761 GGTACC GGTAC^C
MluNI 1941 TGGCCA TGG^CCA
MscI 1941 TGGCCA TGG^CCA
NheI 318 GCTAGC G^CTAGC
NruI 93 TCGCGA TCG^CGA
PflFI 1267 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3004 GAGCTC GAGCT^C
SfaNI 2528 GCATC GCATC(5/9)
SmaI 2729 CCCGGG CCC^GGG
SspBI 2135 TGTACA T^GTACA
StuI 2255 AGGCCT AGG^CCT
Tth111I 1267 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2729 CCCGGG C^CCGGG
XmaI 2729 CCCGGG C^CCGGG
XmnI 2793 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 2599 GAATTC [GA]^AATT[CT]
2877 GAATTT
AcyI 1438 GACGTC G[GA]^CG[CT]C
2482 GGCGTC
AlwNI 1354 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1673 CAGGGGCTG
ApoI 2599 GAATTC [GA]^AATT[CT]
2877 GAATTT
BfaI 319 CTAG C^TAG
1715 CTAG
BlpI 1278 GCTGAGC GC^T[ACGT]AGC
2901 GCTCAGC
Bpu1102I 1278 GCTGAGC GC^T[ACGT]AGC
2901 GCTCAGC
BsaHI 1438 GACGTC G[GA]^CG[CT]C
2482 GGCGTC
BseRI 162 CTCCTC GAGGAG(10/8)
270 GAGGAG
BsgI 1030 CTGCAC GTGCAG(16/14)
2662 GTGCAG
BsiEI 237 CGACCG CG[GA][CT]^CG
1479 CGACCG
BstYI 326 AGATCT [GA]^GATC[CT]
2670 GGATCT
CelII 1278 GCTGAGC GC^T[ACGT]AGC
2901 GCTCAGC
EarI 502 CTCTTC CTCTTC(1/4)
2392 GAAGAG
EcoNI 2862 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2940 CCTCCCCCAGG
EcoT14I 2604 CCAAGG C^C[AT][AT]GG
2735 CCAAGG
HaeII 867 GGCGCT [GA]GCGC^[CT]
1394 GGCGCT
Hin1I 1438 GACGTC G[GA]^CG[CT]C
2482 GGCGTC
Ksp632I 502 CTCTTC CTCTTC(1/4)
2392 GAAGAG
MaeI 319 CTAG C^TAG
1715 CTAG
MflI 326 AGATCT [GA]^GATC[CT]
2670 GGATCT
PshAI 213 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1480 GACCGGAGTC
SmlI 1468 CTCAAG C^T[CT][GA]AG
2695 CTTAAG
StyI 2604 CCAAGG C^C[AT][AT]GG
2735 CCAAGG
XhoII 326 AGATCT [GA]^GATC[CT]
2670 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaI BsaJI BsaWI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I