Restriction map for
pF1KE0308
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI BspEI BspLU11I
BspMI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HpaI KasI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
NarI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 262 GGTACC G^GTACC
AccI 1454 GTAGAC GT^[AC][GT]AC
AcsI 1502 AAATTC [GA]^AATT[CT]
AcyI 89 GGCGTC G[GA]^CG[CT]C
ApaI 573 GGGCCC GGGCC^C
ApoI 1502 AAATTC [GA]^AATT[CT]
Asp718I 262 GGTACC G^GTACC
AspHI 769 GTGCTC G[AT]GC[AT]^C
BamHI 851 GGATCC G^GATCC
BanI 262 GGTACC G^G[CT][GA]CC
BcgI 400 GCAGTGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 645 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 645 GCTGAGC GC^T[ACGT]AGC
BsaHI 89 GGCGTC G[GA]^CG[CT]C
BsiHKAI 769 GTGCTC G[AT]GC[AT]^C
BsmAI 1413 GTCTC GTCTC(1/5)
BsmI 668 GAATGC GAATGC(1/-1)
BspDI 1477 ATCGAT AT^CGAT
BspHI 1083 TCATGA T^CATGA
BsrBI 723 GAGCGG CCGCTC(-3/-3)
BsrDI 290 GCAATG GCAATG(2/0)
BsrI 35 ACTGG ACTGG(1/-1)
BstAPI 406 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 645 GCTGAGC GC^T[ACGT]AGC
ClaI 1477 ATCGAT AT^CGAT
DraIII 123 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 870 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1380 GAAGAG CTCTTC(1/4)
EcoNI 1054 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 941 ATGCAT ATGCA^T
Hin1I 89 GGCGTC G[GA]^CG[CT]C
HindIII 1471 AAGCTT A^AGCTT
KpnI 262 GGTACC GGTAC^C
Ksp632I 1380 GAAGAG CTCTTC(1/4)
KspI 61 CCGCGG CCGC^GG
MaeIII 1119 GTTAC ^GT[ACGT]AC
MslI 1078 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 941 ATGCAT ATGCA^T
NspI 396 GCATGC [GA]CATG^[CT]
RcaI 1083 TCATGA T^CATGA
SacII 61 CCGCGG CCGC^GG
SapI 1380 GAAGAGC GCTCTTC(1/4)
SexAI 512 ACCTGGT A^CC[AT]GGT
SmlI 802 CTCAAG C^T[CT][GA]AG
SphI 396 GCATGC GCATG^C
TaqI 1478 TCGA T^CGA
TthHB8I 1478 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 62 CGCG CG^CG
220 CGCG
BsmFI 58 GTCCC GGGAC(10/14)
282 GTCCC
Bsp1407I 320 TGTACA T^GTACA
1185 TGTACA
BsrFI 86 GCCGGC [GA]^CCGG[CT]
351 GCCGGC
BsrGI 320 TGTACA T^GTACA
1185 TGTACA
BstUI 62 CGCG CG^CG
220 CGCG
BstXI 134 CCATCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
285 CCAGGGCAATGG
BstYI 617 GGATCT [GA]^GATC[CT]
851 GGATCC
Cfr10I 86 GCCGGC [GA]^CCGG[CT]
351 GCCGGC
DpnII 618 GATC ^GATC
852 GATC
DsaI 61 CCGCGG C^C[GA][CT]GG
363 CCGTGG
HaeII 631 AGCGCC [GA]GCGC^[CT]
763 GGCGCT
HgaI 56 GCGTC GACGC(5/10)
90 GCGTC
MboI 618 GATC ^GATC
852 GATC
MflI 617 GGATCT [GA]^GATC[CT]
851 GGATCC
MspA1I 61 CCGCGG C[AC]G^C[GT]G
774 CCGCTG
MvnI 62 CGCG CG^CG
220 CGCG
NaeI 86 GCCGGC GCC^GGC
351 GCCGGC
NdeII 618 GATC ^GATC
852 GATC
NgoMIV 86 GCCGGC G^CCGGC
351 GCCGGC
PleI 674 GACTC GAGTC(4/5)
280 GAGTC
PpuMI 97 AGGACCC [GA]G^G[AT]CC[CT]
868 AGGACCC
Sau3AI 618 GATC ^GATC
852 GATC
SspBI 320 TGTACA T^GTACA
1185 TGTACA
TfiI 1057 GAATC G^A[AT]TC
1157 GATTC
XhoII 617 GGATCT [GA]^GATC[CT]
851 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MboII MnlI MseI MspI MvaI
MwoI NciI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfaNI SfcI
StyI Tru9I TseI Tsp509I TspRI