Restriction map for
pF1KE0303
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI ApaI ApoI AscI Asp718I
AspEI AvrII BbeI BbrPI BbsI
BciVI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaI
BsgI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstEII BstPI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
Eam1105I EarI Eco81I EcoNI EcoO65I
EcoRI EcoRV FseI HgaI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NotI
NruI PacI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccII 912 CGCG CG^CG
Alw44I 1248 GTGCAC G^TGCAC
AlwNI 1472 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 992 AGCGCT AGC^GCT
ApaLI 1248 GTGCAC G^TGCAC
AseI 738 ATTAAT AT^TAAT
AsnI 738 ATTAAT AT^TAAT
Asp700I 95 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1423 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1103 CTCGAG C^[CT]CG[GA]G
AviII 1639 TGCGCA TGC^GCA
BamHI 1656 GGATCC G^GATCC
BanII 126 GAGCTC G[GA]GC[CT]^C
BbvCI 10 GCTGAGG CCTCAGC(-5/-2)
BcgI 329 GCAAGTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1113 TGATCA T^GATCA
BfaI 1079 CTAG C^TAG
BlpI 898 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 898 GCTAAGC GC^T[ACGT]AGC
BseRI 34 GAGGAG GAGGAG(10/8)
BsoBI 1103 CTCGAG C^[CT]CG[GA]G
BspMI 63 GCAGGT ACCTGC(4/8)
BssSI 123 CACGAG CACGAG(-5/-1)
BstAPI 1303 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1488 TTCGAA TT^CGAA
BstUI 912 CGCG CG^CG
BstXI 626 CCACTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 898 GCTAAGC GC^T[ACGT]AGC
DraII 1400 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 232 GACCGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 564 CGGCCG C^GGCCG
EclXI 564 CGGCCG C^GGCCG
Eco47III 992 AGCGCT AGC^GCT
Eco52I 564 CGGCCG C^GGCCG
EcoO109I 1400 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1773 ATGCAT ATGCA^T
FbaI 1113 TGATCA T^GATCA
FspI 1639 TGCGCA TGC^GCA
HincII 808 GTCAAC GT[CT]^[GA]AC
HindII 808 GTCAAC GT[CT]^[GA]AC
MaeI 1079 CTAG C^TAG
MaeII 572 ACGT A^CGT
MspA1I 1472 CAGCTG C[AC]G^C[GT]G
MvnI 912 CGCG CG^CG
NcoI 1652 CCATGG C^CATGG
NheI 1078 GCTAGC G^CTAGC
NsiI 1773 ATGCAT ATGCA^T
NspV 1488 TTCGAA TT^CGAA
PaeR7I 1103 CTCGAG C^TCGAG
PflFI 1423 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1400 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 738 ATTAAT AT^TAAT
PvuII 1472 CAGCTG CAG^CTG
SacI 126 GAGCTC GAGCT^C
ScaI 332 AGTACT AGT^ACT
SexAI 946 ACCAGGT A^CC[AT]GGT
SfcI 429 CTACAG C^T[GA][CT]AG
SfuI 1488 TTCGAA TT^CGAA
SmlI 1103 CTCGAG C^T[CT][GA]AG
StuI 1131 AGGCCT AGG^CCT
Tth111I 1423 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1103 CTCGAG C^TCGAG
XmnI 95 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccIII 222 TCCGGA T^CCGGA
1121 TCCGGA
BalI 80 TGGCCA TGG^CCA
638 TGGCCA
BanI 55 GGCACC G^G[CT][GA]CC
661 GGCACC
BpmI 1560 CTCCAG CTGGAG(16/14)
1213 CTGGAG
BsaWI 222 TCCGGA [AT]^CCGG[AT]
1121 TCCGGA
BseAI 222 TCCGGA T^CCGGA
1121 TCCGGA
BsiEI 231 CGACCG CG[GA][CT]^CG
564 CGGCCG
BsmFI 474 GGGAC GGGAC(10/14)
569 GGGAC
BspEI 222 TCCGGA T^CCGGA
1121 TCCGGA
BspHI 191 TCATGA T^CATGA
368 TCATGA
BstYI 210 AGATCC [GA]^GATC[CT]
1656 GGATCC
FauI 493 CCCGC CCCGC(4/6)
1611 CCCGC
HaeII 524 GGCGCT [GA]GCGC^[CT]
992 AGCGCT
MflI 210 AGATCC [GA]^GATC[CT]
1656 GGATCC
MluNI 80 TGGCCA TGG^CCA
638 TGGCCA
MroI 222 TCCGGA T^CCGGA
1121 TCCGGA
MscI 80 TGGCCA TGG^CCA
638 TGGCCA
MseI 739 TTAA T^TAA
1532 TTAA
RcaI 191 TCATGA T^CATGA
368 TCATGA
Tru9I 739 TTAA T^TAA
1532 TTAA
XcmI 626 CCACTGCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1529 CCATTAAACCTCTGG
XhoII 210 AGATCC [GA]^GATC[CT]
1656 GGATCC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SphI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I