Restriction map for
pF1KE0302
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII AccI AclI AcsI AflII
AgeI AhdI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AvrII
BbeI BbrPI BbvCI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoRI EcoRV FseI
HincII HindII HindIII HpaI KasI
KspI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TthHB8I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 929 GGTACC G^GTACC
AccII 581 CGCG CG^CG
AccIII 395 TCCGGA T^CCGGA
AflIII 420 ACGTGT A^C[GA][CT]GT
Alw44I 274 GTGCAC G^TGCAC
AlwNI 378 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 61 CCTGAGG CC^T[ACGT]AGG
Aor51HI 116 AGCGCT AGC^GCT
ApaLI 274 GTGCAC G^TGCAC
Asp718I 929 GGTACC G^GTACC
AspI 1189 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1133 TGCGCA TGC^GCA
BanI 929 GGTACC G^G[CT][GA]CC
BciVI 287 GTATCC GTATCC(6/5)
BfaI 370 CTAG C^TAG
BsaWI 395 TCCGGA [AT]^CCGG[AT]
BseAI 395 TCCGGA T^CCGGA
BsmBI 550 GAGACG CGTCTC(1/5)
BspEI 395 TCCGGA T^CCGGA
BspHI 698 TCATGA T^CATGA
BsrBI 1249 CCGCTC CCGCTC(-3/-3)
BsrDI 1388 GCAATG GCAATG(2/0)
BstEII 1260 GGTCACC G^GT[ACGT]ACC
BstPI 1260 GGTCACC G^GT[ACGT]ACC
BstUI 581 CGCG CG^CG
BstXI 114 CCAGCGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 61 CCTGAGG CC^T[ACGT]AGG
DraI 496 TTTAAA TTT^AAA
EarI 778 CTCTTC CTCTTC(1/4)
Eco47III 116 AGCGCT AGC^GCT
Eco81I 61 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1260 GGTCACC G^GT[ACGT]ACC
EcoT22I 1158 ATGCAT ATGCA^T
FauI 1181 CCCGC CCCGC(4/6)
FspI 1133 TGCGCA TGC^GCA
KpnI 929 GGTACC GGTAC^C
Ksp632I 778 CTCTTC CTCTTC(1/4)
MaeI 370 CTAG C^TAG
MroI 395 TCCGGA T^CCGGA
MspA1I 451 CAGCTG C[AC]G^C[GT]G
MvnI 581 CGCG CG^CG
NcoI 572 CCATGG C^CATGG
NruI 580 TCGCGA TCG^CGA
NsiI 1158 ATGCAT ATGCA^T
PflFI 1189 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 980 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 451 CAGCTG CAG^CTG
RcaI 698 TCATGA T^CATGA
SexAI 1190 ACCTGGT A^CC[AT]GGT
SfcI 39 CTACAG C^T[GA][CT]AG
SmaI 102 CCCGGG CCC^GGG
StuI 81 AGGCCT AGG^CCT
Tth111I 1189 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 980 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 102 CCCGGG C^CCGGG
XmaI 102 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 564 GACGCC G[GA]^CG[CT]C
1309 GACGCC
BamHI 725 GGATCC G^GATCC
849 GGATCC
BcgI 9 GCAGAGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
884 GCATGGTCATCG
BclI 254 TGATCA T^GATCA
1316 TGATCA
BpmI 1045 CTCCAG CTGGAG(16/14)
1255 CTGGAG
BsaBI 1068 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1317 GATCAGCATC
BsaHI 564 GACGCC G[GA]^CG[CT]C
1309 GACGCC
BseRI 96 CTCCTC GAGGAG(10/8)
64 GAGGAG
BsmAI 12 GAGAC GTCTC(1/5)
550 GAGAC
BspMI 932 ACCTGC ACCTGC(4/8)
974 ACCTGC
DraII 237 GGGTCCC [GA]G^G[ACGT]CC[CT]
1351 GGGACCT
DsaI 47 CCACGG C^C[GA][CT]GG
572 CCATGG
EcoO109I 237 GGGTCCC [GA]G^G[ACGT]CC[CT]
1351 GGGACCT
FbaI 254 TGATCA T^GATCA
1316 TGATCA
HgaI 564 GACGC GACGC(5/10)
1309 GACGC
Hin1I 564 GACGCC G[GA]^CG[CT]C
1309 GACGCC
MamI 1068 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1317 GATCAGCATC
NspI 590 ACATGC [GA]CATG^[CT]
1156 ACATGC
PpuMI 237 GGGTCCC [GA]G^G[AT]CC[CT]
1351 GGGACCT
Tsp509I 938 AATT ^AATT
1408 AATT
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp45I TspRI XhoII